中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (29): 4613-4619.doi: 10.3969/j.issn.2095-4344.2811

• 骨组织构建 bone tissue construction • 上一篇    下一篇

基于网络药理学分析三七治疗股骨头坏死的免疫炎症分子机制

农  焦1,曾  平2,李  娟2,刘金富1,李金溢2   

  1. 1广西中医药大学,广西壮族自治区南宁市  530001;2广西中医药大学第一附属医院,广西壮族自治区南宁市  530023
  • 收稿日期:2019-08-27 修回日期:2019-09-06 接受日期:2019-10-26 出版日期:2020-10-18 发布日期:2020-09-11
  • 通讯作者: 曾平,博士,教授,广西中医药大学第一附属医院,广西壮族自治区南宁市 530023
  • 作者简介:农焦,男,1991年生,广西壮族自治区百色市人,壮族,在读硕士,主要从事中医药用于四肢骨病与创伤的防治研究。
  • 基金资助:
    广西壮族自治区级硕士研究生创新项目(YCSW2019172)

Immunoinflammatory mechanism of Panax notoginseng in the treatment of osteonecrosis of the femoral head based on network pharmacology

Nong Jiao1, Zeng Ping2, Li Juan2, Liu Jinfu1, Li Jinyi2   

  1. 1Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China; 2First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China
  • Received:2019-08-27 Revised:2019-09-06 Accepted:2019-10-26 Online:2020-10-18 Published:2020-09-11
  • Contact: Zeng Ping, MD, Professor, First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China
  • About author:Nong Jiao, Master candidate, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China
  • Supported by:
    the Master’s Degree Graduate Innovation Project of Guangxi Zhuang Autonomous Region, No. YCSW2019172

摘要:

文题释义:
三七:为五加科植物三七Panax notoginseng (Burk.)F.H.Chen的干燥根和根茎,具有通脉行瘀、和营止血、行瘀血而敛新血的功效。
网络药理学:融合了系统生物学和多向药理学的思想,从整体角度探索药物、靶点、疾病之间的相互关联性。
背景:近年来,临床上运用中药治疗早中期股骨头坏死获得了比较满意的疗效,但与西药相比,中药成分复杂、靶点和机制不清。网络药理学是一门新兴且热门的学科,网络药理学的整体性、系统性和注重药物间相互作用的特点与中医药学的基本特点相吻合, 符合中医药对疾病本质的认识。
目的:运用网络药理学方法筛选三七主要活性成分,构建活性化合物-靶点网络,对其治疗股骨头坏死的分子机制进行科学阐释。
方法:利用TCMSP数据库筛选出三七的作用靶点,OMIM、GeneCards数据库筛选出股骨头坏死疾病的作用相关靶点。利用STRING数据库进行蛋白互作网络分析,Cytoscape构建蛋白相互作用网络,利用Cytoscape软件构建化合物-靶点网络并进行网络拓扑学分析,通过生物学信息注释数据库(DAVID)分析靶点基因功能及信号通路,利用ClueGO插件进行GO分析和KEGG信号通路富集分析。
结果与结论:筛选出8个三七化学活性成分,98个三七药物作用于股骨头坏死的潜在靶点。GO富集结果表明三七主要涉及细胞过程、代谢过程、对应激的应答等生物过程,KEGG靶点分析表明三七主要通过Toll样受体信号通路、磷脂酰肌醇3激酶/蛋白激酶B(PI3K-AKT)信号通路、肿瘤坏死因子信号通路、核转录因子κB信号通路、氨基末端激酶/信号转导与转录激活子(JAK-STAT)信号通路影响来发挥免疫炎症作用。三七治疗股骨头坏死的过程体现了中药多成分-多靶点-多途径的作用特点,为三七抗免疫炎症作用机制的研究甚至进一步开展中药复方治疗疾病提供了新思路和新方法。
ORCID: 0000-0001-5801-8391(曾平)

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程

关键词: 三七, 股骨头坏死, 网络药理学, 生物信息学, 免疫炎症作用机制

Abstract:

BACKGROUND: In recent years, traditional Chinese medicine has obtained satisfactory results in the treatment of early and middle-stage osteonecrosis of the femoral head. Compared with western medicine, traditional Chinese medicine has complex components, unclear targets and mechanisms. Network pharmacology is a new and popular discipline, which is consistent with the traditional Chinese medicine in terms of holistic, systemic, and drug-focused characteristics, meeting the understanding of the nature of diseases in traditional Chinese medicine.

OBJECTIVE: To screen the main active components of Panax notoginseng by network pharmacology, construct the active compound-target network, and elucidate the molecular mechanism of its therapeutic effect on osteonecrosis of the femoral head.

METHODS: The targets of Panax notoginseng were screened by TCMSP database, and the targets related to osteonecrosis of the femoral head were screened by OMIM and GeneCards databases. The protein interaction network was analyzed by STRING database, the protein interaction network was constructed by Cytoscape, the compound-target network was constructed by Cytoscape software, and the network topology was analyzed. The target gene function and signal pathway were analyzed by biological information annotation database (DAVID), and the GO analysis and KEGG signal pathway enrichment analysis were carried out using the ClueGO plug-in.

RESULTS AND CONCLUSION: There were 8 active components of Panax notoginseng, involving 98 targets related to osteonecrosis of the femoral head. Panax notoginseng was mainly involved in cell process, metabolic process, response to stress and other biological processes. Panax notoginseng played an anti-inflammatory role by regulating Toll-like receptor, PI3K-AKT, tumor necrosis factor, nuclear factor-κB, and JAK-STAT signal pathways. This study reflects the characteristics of components of Panax notoginseng-target-pathway, and provides new ideas and methods for further research on the anti-inflammatory mechanism of Panax notoginseng and even for disease treatment with compound Chinese medicine.

Key words: Panax notoginseng, osteonecrosis of the femoral head, network pharmacology, bioinformatics, mechanism of immune inflammation

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