中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (11): 1987-1991.doi: 10.3969/j.issn.1673-8225.2010.11.022

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

毛囊隆突区β神经生长因子水平对乳鼠烫伤创面愈合的影响

曾 俊1，陈登榜1，代吕霞1，林 森2   

  1. 1成都医学院临床医学实验教学中心，四川省成都市  610083；
    2成都医学院人体解剖与组织胚胎学教研室，四川省成都市  610083
  • 出版日期:2010-03-12 发布日期:2010-03-12
  • 通讯作者: 林 森，硕士，讲师，成都医学院人体解剖与组织胚胎学教研室，四川省成都市 610083 lsroger@163.com
  • 作者简介:曾 俊，女，1982年生，四川省成都市人，汉族， 2005年成都医学院毕业，助理实验师，主要从事糖尿病皮肤溃疡机制研究。 janezj6@hotmail.com
  • 基金资助:

    四川省教育厅青年基金资助项目(07ZB016)

Influence of beta nerve growth factor in vibrissa follicle bulge region on the burn wound healing of neonatal rats

Zeng Jun1, Chen Deng-bang1, Dai Lü-xia1, Lin Sen2   

  1. 1Clinic Experiment Teaching Center of Chengdu Medical College, Chengdu  610083, Sichuan Province, China;
    2Department of Human Anatomy, Histology and Embryology, Chengdu Medical College, Chengdu  610083, Sichuan Province, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Lin Sen, Master, Lecturer, Department of Human Anatomy, Histology and Embryology, Chengdu Medical College, Chengdu 610083, Sichuan Province, China lsroger@163.com
  • About author:Zeng Jun, Assistant laboratory technician, Clinic Experiment Teaching Center of Chengdu Medical College, Chengdu 610083, Sichuan Province, China janezj6@hotmail.com
  • Supported by:

    the Youth Fund Project of Education Department of Sichuan Province, No. 07ZB016*

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摘要：

背景：毛囊干细胞在受到外界环境刺激作用下，例如创伤，化学伤等，其理化性质及细胞状态会有所变化。但是从目前文献来看，涉及其在皮肤烫伤中变化的研究的比较少。
目的：初步观察大鼠烫伤模型中，毛囊隆突区β神经生长因子的表达变化以及对烫伤组织愈合的影响。
方法：随机选取30只乳鼠，采用90 ℃水温持续3 s时间的方式制作大鼠触须部表皮组织浅Ⅱ度烫伤模型，造模后第12，24，36，48，60小时对毛囊隆突区进行分离，并进行匀浆，以同窝新生6只乳鼠正常唇部组织作为对照。采用Brandford法进行总蛋白检测及ELISA定量检测β神经生长因子。免疫组织化学进行神经生长因子的定性半定量检测。
结果与结论：在烫伤后各时间点检测的β神经生长因子与总蛋白比值中，β神经生长因子的表达在烫伤后36 h开始后增高，60 h达到峰值。组织切片中同样发现这一规律。通过图像软件分析表明，提取β神经生长因子蛋白检测与组织水平β神经生长因子表达一致。提示烫伤后12~60 h时间段内，烫伤后60 h隆突区表达的β神经生长因子达到峰值，对烫伤愈合的修复作用也达到峰值。隆突区是毛囊干细胞的巢，此部位β神经生长因子表达增强可能对毛囊干细胞的分化和组织修复具有一定的积极意义。

关键词: 毛囊隆突区, &beta, 神经生长因子, 烫伤, 创面愈合, 皮肤组织工程, 乳鼠

Abstract:

BACKGROUND: The surroundings stimulation, such as wound and chemical injury, will result in changes of hair follicle stem cells (HFSC). Up to date, few articles indicate the effect of burn on HFSCs. 
OBJECTIVE: To investigate the changes of β-nerve growth factor (β-NGF) level in vibrissa follicle bulge region and its effect on the healing of neonatal rats.
METHODS: A total of 30 neonatal rats were selected to prepare burn models by giving 90 ℃ boiled tap water for 3 seconds, and vibrissa follicle bulge was separated at 12, 24, 36, 48 and 60 hours postoperatively. The remained 6 neonatal rats were served as controls. Total protein concentration was measured by Brandford method. The expression of β-NGF was detected by ELISA. Meanwhile, the tissues in each time points were collected for frozen section and immunohistochemistry.
RESULTS AND CONCLUSION: Among the tested total protein, the expression of the β-NGF increased after 36 hours, reached a peak at 60 hours after burn. The identical changes were found in tissue slices. The image analysis demonstrated that the β-NGF protein detection was coincident with β-NGF expression in tissues. During 12 hours to 60 hours after burn, the expression of the β-NGF in the bulge area peaked at 60 hours; simultaneously, the repairing effect of healing also reached a peak. Bulge area is a nest of HFSC, the enhanced expression of β-NGF may have some positive significance to the differentiation of the HFSC and tissue repair.

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