中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5165-5169.doi: 10.3969/j.issn.2095-4344.2012.28.008

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

低功率激光修复末端病大鼠跟腱胶原

沈勇伟1，史晓伟2   

  1. 1苏州大学体育学院，江苏省苏州市 215021；
    2海南师范大学体育系，海南省海口市 570100
  • 收稿日期:2011-11-01 修回日期:2011-12-17 出版日期:2012-07-08 发布日期:2012-07-08
  • 作者简介:沈勇伟☆，男，1961年生，江苏省苏州市人，汉族，苏州大学在读博士，副教授，主要从事运动生理生化研究。 shenyongwei0719@163.com

Low level laser for repair of collagen in the tendon of rats with enthesiopathy

Shen Yong-wei1, Shi Xiao-wei2   

  1. 1Institute of Physical Education, Soochow University, Suzhou 215021, Jiangsu Province, China;
    2Department of Physical Education, Hainan Normal University, Haikou 570100, Hainan Province, China
  • Received:2011-11-01 Revised:2011-12-17 Online:2012-07-08 Published:2012-07-08
  • About author:Shen Yong-wei☆, Studying for doctorate, Associate professor, Institute of Physical Education, Soochow University, Suzhou 215021, Jiangsu Province, China shenyongwei0719@163.com

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409

被引次数

8

摘要：

背景：低功率激光疗法能对生物体产生特定的刺激作用(光生物调节作用)，一般在不引起温度上升的情况下能引起某些生物学效应。
目的：观察低功率激光对末端病大鼠跟腱组织中羟脯氨酸含量、Ⅰ型胶原基因表达的影响。
方法：将96只Wistar大鼠随机分组：正常对照组不进行任何干预；造模组以电刺激跳跃法刺激大鼠足部，建立末端病大鼠模型；激光照射组建立末端病大鼠模型后于跟腱处给予低功率激光照射治疗。
结果与结论：与正常对照组比较，末端病模型大鼠跟腱组织中羟脯氨酸含量与Ⅰ型胶原水平明显下降(P < 0.05)。经低功率激光照射治疗后，末端病模型大鼠跟腱羟脯氨酸含量增加，Ⅰ型胶原合成在转录水平上的表达升高，且这种作用在造模7 d后开始出现，14 d时效果非常明显。表明低功率激光可以提高末端病模型大鼠羟脯氨酸合成量，促进胶原的代谢，在跟腱早期愈合中有一定的促进作用。

关键词: 低功率激光, 末端病, 跟腱, 羟脯氨酸, Ⅰ型胶原mRNA

Abstract:

BACKGROUND: Low level laser therapy (LLLT) has the specific stimulation effect on organism (photobiomodulation). In general case, it can produce some biological effects without temperature increase.
OBJECTIVE: To observed the effect of low level laser on contents of hydroxyproline and gene expression of collagenⅠin the tendon of rats with enthesiopathy.
METHODS: Ninety-six male Wistar rats were randomly divided into two groups. The control group was without ant treatment. Rats in the model group were stimulated to the feet with electrostimulating jumping method to build the model of enthesiopathy. Rats in the LLLT group were treated with LLLT to the tendon after model building.
RESULTS AND CONCLUSION: Compared with the control group, the contents of hydroxyproline and gene expression of collagenⅠ in the tendon of rats with enthesiopathy were significantly decreased (P < 0.05). After LLLT, low level laser could increase the contents of hydroxyproline and gene expression of collagen I in the tendon of rats with enthesiopathy, and this started from 7 days and was obviously increased at 14 days. It is indicated that low level laser can improve the contents of hydroxyproline and the metabolism of collagenⅠin the tendon of rats with enthesiopathy, and can promote the healing of tendon in early stage.

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