中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (29): 5321-5326.doi: 10.3969/j.issn. 2095-4344.2012.29. 001

• 纳米生物材料 nanobiomaterials •    下一篇

壳聚糖/纳米羟基磷灰石生物驻极体屏障膜的骨引导性

敖丹婷1，王艳颖2，王 萍2，屈依丽1，满 毅2，陈治清1   

  1. 1四川大学口腔疾病国家重点实验室，四川省成都市 610041；
    2四川大学华西口腔医学院种植中心，四川省成都市 610041
  • 收稿日期:2011-11-25 修回日期:2011-12-26 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 陈治清，教授，博士生导师，四川大学口腔疾病国家重点实验室，四川省成都市 610041 chenzhiqing40@126.com
  • 作者简介:敖丹婷☆，女，1986年生，四川省攀枝花市人，汉族，四川大学华西口腔医学院口腔修复科在读博士，主要从事口腔修复学的临床及基础研究。 kathy123@ sina.com

Osteoinductivity of chitosan/nano-hydroxyapatite bioelectret barrier membranes

Ao Dan-ting1, Wang Yan-ying2, Wang Ping2, Qu Yi-li1, Man Yi2, Chen Zhi-qing1   

  1. 1State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, Sichuan Province, China;
    2Center of Implantation, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2011-11-25 Revised:2011-12-26 Online:2012-07-15 Published:2012-07-15
  • Contact: Chen Zhi-qing, Professor, Doctoral supervisor, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, Sichuan Province, China chenzhiqing40@ 126.com
  • About author:Ao Dan-ting☆, Studying for doctorate, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, Sichuan Province, China kathy123@sina.com

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482

被引次数

3

摘要：

背景：当前应用于引导骨再生的屏障膜不具备骨引导性。
目的：探讨极化后的复合生物膜对成骨细胞相容性和活性的影响。
方法：采用栅控电晕法制备了壳聚糖/羟基磷灰石复合生物驻极体膜，检测驻极体膜上的表面电位衰减及接种成骨细胞后细胞的增殖分化情况。
结果与结论：驻极体膜上的表面电位在第1天内急速衰减，但在接下来的9 d内衰减速度逐渐减慢。相较于非极化膜，驻极体膜上的细胞边界明显，有较多的伪足，细胞增殖能力更强，碱性磷酸酶的表达也更高(P < 0.05)，但肌动蛋白直接免疫荧光结果无显著差异。结果显示该驻极体膜是一种新型的拥有骨引导性的屏障膜，可促进成骨细胞的增殖和分化。

关键词: 生物驻极体, 屏障膜, 复合材料, 壳聚糖, 纳米羟基磷灰石

Abstract:

BACKGROUND: Currently, the barrier membranes that applied in guided bone regeneration have no osteoinductivity.
OBJECTIVE: To explore the effects of composite bioelectret membranes after polarization on compatibility and activity of osteoblasts.
METHODS: Chitosan/nano-hydroxyapatite bioelectret composite membranes were prepared by grid-controlled corona charge. The surface potential decay of the membranes and the proliferation and differentiation of osteoblasts seeded on them were evaluated.
RESULTS AND CONCLUSION: The surface potential was obviously reduced in 1 day, but the speed of decay slowed gradually during the following 9 days. Compared with the unpolarized group, the osteoblasts on the bioelectret membranes had clearer boundaries and more pseudopodia. Moreover, the proliferation ability and alkaline phosphatase activity of the cells seeded on bioelectret membranes were higher than those of the unpolarized group (P < 0.05). But the direct immunofluorescence showed no significant difference in actin between the two groups. In conclusion, bioelectret mebrane is a new type of barrier membrane with osteoinductivity and can improve the proliferation and differentiation of osteoblasts.

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