中国组织工程研究

中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (10): 1515-1520.doi: 10.3969/j.issn.2095-4344.2235

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

两种引导组织再生膜骨修复效果的比较

尹  颖1，2，王家齐1，戴小寒1，李毅萍1，张学慧2，3   

  1. 1中南大学湘雅口腔医学院修复科，湖南省长沙市  410078；北京大学口腔医学院，2口腔材料研究室，3口腔医疗器械检验中心，北京市  100081
  • 收稿日期:2019-03-14 修回日期:2019-03-23 接受日期:2019-05-24 出版日期:2020-04-08 发布日期:2020-02-14
  • 通讯作者: 李毅萍，副主任医师，中南大学湘雅口腔医学院修复科，湖南省长沙市 410078
  • 作者简介:尹颖，女，1994 年生，汉族，湖南省益阳市人，中南大学湘雅口腔医(学)院在读硕士，主要从事口腔硬组织修复材料研究工作。
  • 基金资助:
    湖南省卫生计生委科研计划课题(C2017009)

A comparative study on the bone repair effects of two kinds of tissue regeneration membranes

Yin Ying1, 2, Wang Jiaqi1, Dai Xiaohan1, Li Yiping1, Zhang Xuehui2, 3   

  1. 1Department of Prosthodontics, Xiangya School of Stomatology, Central South University, Changsha 410078, Hunan Province, China; 2Department of Dental Materials, 3Dental Medical Devices Testing Center, Peking University Hospital of Stomatology, Beijing 100081, China
  • Received:2019-03-14 Revised:2019-03-23 Accepted:2019-05-24 Online:2020-04-08 Published:2020-02-14
  • Contact: Li Yiping, Associate chief physician, Department of Prosthodontics, Xiangya School of Stomatology, Central South University, Changsha 410078, Hunan Province, China
  • About author:Yin Ying, Master candidate, Department of Prosthodontics, Xiangya School of Stomatology, Central South University, Changsha 410078, Hunan Province, China; Department of Dental Materials, Peking University Hospital of Stomatology, Beijing 100081, China
  • Supported by:
    Scientific Research Project of Hunan Health and Family Planning Commission (Project No. C2017009)

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摘要：

文题释义：
压电效应：指晶体材料受到压力作用时会在两端面间出现电压。1880年，法国物理学家P.居里和J.居里兄弟发现，把重物放在石英晶体上后晶体某些表面会产生电荷，电荷量与压力呈比例。
压电常数：是压电体将机械能与电能相互转变的转换系数，主要反映了压电材料弹性(机械)性能与介电性能之间的耦合关系。


背景：课题组前期研究证实，含体积分数5%钛酸钡纳米颗粒的钛酸钡/聚偏氟乙烯三氟乙烯压电纳米复合膜可明显促进骨髓间充质干细胞的黏附、生长、成骨分化及骨缺损修复，然而针对临床应用，该材料作为引导组织再生膜与现有临床上不可降解膜产品的骨修复效果有何差异尚不清楚。

目的：对比考察钛酸钡/聚偏氟乙烯三氟乙烯压电纳米复合膜材料与商用聚四氟乙烯膜修复大鼠颅骨临界尺寸缺损的效果。

方法：采用溶液浇铸法制备出含体积分数5%钛酸钡纳米颗粒的钛酸钡/聚偏氟乙烯三氟乙烯压电纳米复合膜，经过电晕极化处理后使其表面带电。通过扫描电镜、原子力显微镜和水接触角测量仪检测钛酸钡/聚偏氟乙烯三氟乙烯压电纳米复合膜与聚四氟乙烯膜的表面形貌、表面粗糙度及表面亲疏水性。在SD大鼠(购自北京大学口腔医学院实验动物中心)颅骨矢状缝两侧制作直径为5 mm的全厚骨缺损，左侧覆盖聚四氟乙烯膜(对照组)，右侧覆盖钛酸钡/聚偏氟乙烯三氟乙烯压电纳米复合膜(实验组)，术后4，12周，利用Micro-CT和组织学方法评价材料覆盖大鼠颅骨缺损的骨修复情况。实验已经通过北京大学口腔医学院实验动物伦理委员会讨论批准。

结果与结论：①钛酸钡/聚偏氟乙烯三氟乙烯压电纳米复合膜表面平整致密，钛酸钡纳米颗粒均匀分布在基体内；聚四氟乙烯膜由疏松的粗大纤维组成；钛酸钡/聚偏氟乙烯三氟乙烯压电纳米复合膜的表面粗糙度低于聚四氟乙烯膜(P < 0.001)，亲水性优于聚四氟乙烯膜(P < 0.001)；②Micro-CT和组织学检测显示，术后4周时，两组缺损处均有新骨生成，但实验组缺损中央有明显的新骨生成；术后12周时，两组缺损均已愈合，但实验组新骨成熟程度高于对照组；③结果表明，钛酸钡/聚偏氟乙烯三氟乙烯压电纳米复合膜可能作为引导组织再生膜。

ORCID: 0000-0002-6993-5627(尹颖)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 仿生电活性, 引导组织再生膜, 压电聚合物, 植入材料, 生物材料, 压电常数, 骨再生, 骨修复, 临界尺寸

Abstract:

BACKGROUND: Our previous study confirmed that BTO/P(VDF-TrFE) piezoelectric nanocomposite membrane containing 5%BTO (BaTO3, BTO) nanoparticles could significantly promote bone marrow mesenchymal stem cells (BMSCs) adhesion, growth, osteogenic differentiation and repair of bone defects due to its bionic potential. However, for clinical applications, it is not clear whether there is any difference between the bone repair effect of this material as a guide for tissue regeneration and that of the existing clinically non-degradable membrane products.

OBJECTIVE: To compare the effects of BTO/P(VDF-TrFE) piezoelectric nano-composite membrane materials with commercial PTFE membrane in repairing critical-sized defects of SD rat skull.

METHODS: BTO/P(VDF-TrFE) piezoelectric nanocomposite membrane containing 5% BTO nanoparticles was prepared by solution casting method. Scanning electron microscope, atomic force microscope and water contact angle measuring instrument were used to observe the surface morphology, measure the surface roughness and surface hydrophilicity and hydrophobicity of the material. In SD rats (purchased from the Laboratory Animal Center, Beijing University Hospital of Stomatology, China), a 5-mm sized full-thick bone defect was made on both sides of the sagittal suture of the skull. The left bone defect was covered with PTFE membrane (control group). The right bone defect was covered with BTO/P(VDF-TrFE) piezoelectric nano-composite membrane (experimental group). At postoperative 4 and 12 weeks, micro-CT and histological methods were used to evaluate the repair of the skull defect in rats. This study was approved by Animal Ethics Committee, Beijing University Hospital of Stomatology.

RESULTS AND CONCLUSION: The piezoelectric nanocomposite membrane had smooth and dense surface and BTO nanoparticles were evenly distributed. PTFE membrane was composed of loose coarse fibers. The piezoelectric nanocomposite membrane had lower surface roughness (P < 0.001) and higher hydrophilicity (P < 0.001) than PTFE membrane. Micro-CT and histological results showed that at 4 weeks after surgery, new bone formation was found in both groups, but new bone formation was more obvious in the center of the defect in the experimental group than in the control group. At 12 weeks after surgery, bone defects healed in both groups, but the maturity of newly formed bone in the experimental group was greater than that in the control group. These results suggest that BTO/P(VDF-TrFE) piezoelectric nanocomposite membrane containing 5%BTO (BaTO3, BTO) nanoparticles can be used as a membrane guiding tissue regeneration.

Key words: bionic electroactivity, tissue regeneration-guiding membrane, piezoelectric polymer, implant material, biomaterials, piezoelectric constant, bone regeneration, bone repair, critical dimension

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