中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (14): 2147-2155.doi: 10.3969/j.issn.2095-4344.1672

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

缓释左氧氟沙星三维丝素蛋白/壳聚糖/纳米羟基磷灰石复合骨组织工程支架材料的制备与表征

叶 鹏，骆付丽，刘安平，段海真，胡 权，黄文金，程 云，喻安永   

  1. 遵义医学院附属医院急救创伤病区，贵州省遵义市 563003
  • 收稿日期:2018-09-29
  • 通讯作者: 喻安永，博士，教授，主任医师，硕士生导师，遵义医学院附属医院急救创伤病区，贵州省遵义市 563003
  • 作者简介:叶鹏，男，1988年生，贵州省遵义市人，汉族，博士，主治医师，主要从事骨关节创伤方面的研究。
  • 基金资助:

    国家自然科学基金地区科学基金(A304)；国家自然科学基金地区科学基金(A226)，项目负责人：喻安永

Preparation and characterization of sustained-release levofloxacin bone tissue-engineered three-dimensional silk fibroin/chitosan/nano-hydroxyapatite scaffold

Ye Peng, Luo Fuli, Liu Anping, Duan Haizhen, Hu Quan, Huang Wenjin, Cheng Yun, Yu Anyong   

  1. Department of Trauma Emergence, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China
  • Received:2018-09-29
  • Contact: Yu Anyong, MD, Professor, Chief physician, Master’s supervisor, Department of Trauma Emergence, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China
  • About author:Ye Peng, MD, Attending physician, Department of Trauma Emergence, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. A304 and A226 (to YAY)

PDF

81

摘要：

文章快速阅读：

 

文题释义：
药物缓释系统：是将药物与具有良好生物相容性的材料以物理或者化学方式结合，使其在人体内以扩散、渗透等方式在局部均匀持续释放，它能控制药物释放速率和周期，并能使药物达到人体特定的靶部位。一种良好的药物缓释系统应该具有较大的载药量和较高的包封率，以减少载体材料和药物的用量。药物缓释载体材料与药物的结合方式一般可以分为2种：物理结合和化学结合。
冷冻干燥技术：冷冻技术使分散在含有3种材料均质溶液中的水形成冰晶，经过负压干燥后，在冰晶最初存在的地方形成了带有孔隙的支架。此种技术最大限度的减少了其他化学试剂的使用，避免了材料理化及生物特性的改变。
 
 
背景：课题组前期实验成功制备了三维丝素蛋白/壳聚糖/纳米羟基磷灰石复合骨组织工程支架材料。
目的：制备缓释左氧氟沙星的三维丝素蛋白/壳聚糖/纳米羟基磷灰石复合骨组织工程支架材料，探讨其机械性能、物理特性和化学构成及抗生素缓释能力。
方法：采用乳化固定过滤方法制备左氧氟沙星/壳聚糖载药微球，其中左氧氟沙星与壳聚糖的质量比为3/1。将5，7.5，10 g的载药微球分别加入质量分数2%的丝素蛋白/壳聚糖/纳米羟基磷灰石混合溶液中，通过冷冻干燥化学交联得到负载抗生素支架。对负载抗生素的支架进行扫描电镜观察、化学成分分析、药物缓释性能及力学、孔隙率、吸水膨胀率、热水溶失率检测。

结果与结论：①扫描电镜显示，支架内壁可见载药微球，并且随着载药微球质量的增加，负载抗生素支架的空隙密度逐渐减小；②能谱分析显示，3种负载抗生素支架均含有丰富的钙离子、磷离子；③3种负载抗生素支架的释放趋势相同，在前3 d释放大于50%，呈突释效应，而后进入相对平稳的释放阶段；负载10 g载药微球支架的药物释放速率最慢，负载5 g载药微球支架的药物释放速率最快；④随着载药微球质量的增加，负载抗生素支架的抗压能力与抗牵张能力逐渐增加，孔隙率、平均孔径、吸水膨胀率逐渐减小，热水溶失率逐渐增加；⑤结果表明，采用冷冻干燥化学交联法可合成负载氧氟沙星的三维骨组织工程支架，其具有良好的缓释性能、抗压抗压缩能力、吸水率及热水溶失率。

ORCID: 0000-0002-4476-7604(叶鹏)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 左氧氟沙星, 骨组织支架, 壳聚糖, 羟基磷灰石, 骨缺损, 生物材料

Abstract:

BACKGROUND: Preliminary study has prepared the three-dimensional silk fibroin/chitosan/nano-hydroxyapatite scaffold successfully.

OBJECTIVE: To explore the mechanical properties, physical characteristics, chemical composition and antibiotic sustained-release ability of three-dimensional silk fibroin/chitosan/nano-hydroxyapatite scaffold loaded with levofloxacin.
METHODS: Levofloxacin/chitosan (3:1) microspheres were constructed by emulsion settlement filter method. 5, 7.5 and 10 g of microspheres were added into 2% of silk fibroin/chitosan/nano-hydroxyapatite mixed solution through freeze drying and chemical cross-linking to obtain the scaffolds loaded with antibiotics. The scaffolds loaded with antibiotics underwent scanning electron microscope observation, and chemical composition analysis. The sustained release, mechanical properties, porosity, water absorption expansion rate and hot water soluble loss rate were detected.
RESULTS AND CONCLUSION: (1) Scanning electron microscope observed that there were drug microspheres at the inner wall of the scaffold, and the voidage was decreased with mass of microspheres increasing. (2) Energy spectrum analysis showed that the three kinds of scaffolds were rich in calcium and phosphonium ions. (3) The three kinds of scaffolds showed the same releasing trend, which presented with sudden-release effect at the former 3 days (release > 50%), and then tended to be stable. The release rate was the slowest in the scaffold loaded with 10 g of microscopes, and the rapidest in the scaffold loaded with 5 g of microscopes. (4) With the mass of microspheres increasing, there was an increase in the compressive and tension abilities and hot water soluble loss rate, and a decrease in the porosity, mean pore size and water absorption expansion rate. (5) These results indicate that the three-dimensional tissue-engineered scaffold loaded with levofloxacin is constructed successfully by freeze drying and chemical cross-linking method, which holds good sustained-release effect and compressive ability, water absorption expansion rate and hot water soluble loss rate.

Key words: Ofloxacin, Silk, Chitosan, Hydroxyapatites, Tissue Engineering

中图分类号: