中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (10): 1514-1519.doi: 10.3969/j.issn.2095-4344.1611

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

β-磷酸三钙复合改良型富血小板纤维蛋白促成骨：影像学及免疫组织化学分析

郑 锐1，2，谢 静1，芦 帅3，孙 勇1，2   

  1. 1西南医科大学口腔医学院，四川省泸州市 646000；2四川口腔医院，四川省成都市 610031；3解放军成都军区机关医院口腔科，四川省成都市 610031
  • 收稿日期:2018-10-13 出版日期:2019-04-08 发布日期:2019-04-08
  • 通讯作者: 孙勇，教授，主任医师，硕士生导师，西南医科大学口腔医学院，四川省泸州市 646000；四川口腔医院，四川省成都市 610031
  • 作者简介:郑锐，女，1991年生，四川省绵阳市人，汉族，西南医科大学在读硕士，医师，主要从事口腔种植义齿的基础与临床的研究。
  • 基金资助:

    军事口腔医学国家重点实验室项目(2016KB01)，项目负责人：孙勇

Beta-tricalcium phosphate combined with advanced platelet-rich fibrin contributes to bone regeneration: X-ray and immunohistochemical analysis

Zheng Rui1, 2, Xie Jing1, Lu Shuai3, Sun Yong1, 2   

  1. 1School of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Sichuan Stomatological Hospital, Chengdu 610031, Sichuan Province, China; 3Department of Stomatology, Institutional Hospital of the Chengdu Military Region, Chengdu 610031, Sichuan Province, China
  • Received:2018-10-13 Online:2019-04-08 Published:2019-04-08
  • Contact: Sun Yong, Professor, Chief physician, Master’s supervisor, School of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Sichuan Stomatological Hospital, Chengdu 610031, Sichuan Province, China
  • About author:Zheng Rui, Master candidate, Physician, School of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Sichuan Stomatological Hospital, Chengdu 610031, Sichuan Province, China
  • Supported by:

     the National Key Laboratory Project for Military Stomatology, No. 2016KB01 (to SY)

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摘要：

文章快速阅读：

 

文题释义：
骨引导：骨缺损周围的成骨细胞和破骨细胞为终末分化的细胞，无迁徙能力。当缺损大小超过临界值时，即临界性骨缺损，必须利用骨替代材料维持骨缺损处的空间，引导血管原细胞和骨原细胞向多孔的材料表面迁移、长入，同时骨替代材料逐步降解，最后全部由新生骨替换。 
骨诱导：在相关骨诱导因子如骨形成蛋白的作用下，诱导骨缺损区的间充质细胞、成纤维细胞等向成骨细胞分化，从而有利于新生骨组织形成。因此，实现骨诱导需要具备3个基本要素：可溶性骨诱导信号；能对骨形成诱导信号作出反应的细胞；富含并释放生长因子和细胞的载体。
 
 
背景：目前研究证明多种骨替代材料与血液衍生物联合应用均具有更好的成骨作用。现阶段国内外均未见关于β-磷酸三钙与改良型富血小板纤维蛋白复合应用于骨缺损修复的相关报道。
目的：观察β-磷酸三钙复合改良型富血小板纤维蛋白用于骨缺损修复的成骨特点及效果。
方法：将39只日本大耳兔随机分为4组，均在兔双侧后肢股骨髁外侧面制备6.0 mm×8.0 mm的柱状临界性骨缺损，改良型富血小板纤维蛋白组(n=12)骨缺损中填入兔自体改良型富血小板纤维蛋白碎屑，β-磷酸三钙组(n=12)填入β-磷酸三钙颗粒，复合组(n=12)填入β-磷酸三钙颗粒与兔自体改良型富血小板纤维蛋白碎屑的等质量混合物，空白组(n=3)不填入任何材料。术后1，2，3个月获取完整股骨段，拍摄X射线片后进行破骨细胞分化因子、骨保护素蛋白免疫组织化学分析。

结果与结论：①X射线显示随着时间的推移，改良型富血小板纤维蛋白组圆形透射影从边缘向中心密度逐渐增高，β-磷酸三钙组和复合组圆形阻射影从边缘向中心密度逐渐降低，最后密度都接近周围天然骨或者与周围天然骨一致；②免疫组织化学显示，4组均有破骨细胞分化因子、骨保护素蛋白阳性表达，相同时间点下破骨细胞分化因子与骨保护素蛋白表达的平均吸光度值比较为：复合组>β-磷酸三钙组>改良型富血小板纤维蛋白组>空白组(P均<0.05)；③结果表明，β-磷酸三钙与改良型富血小板纤维蛋白复合应用时的成骨效果优于二者单独应用。

ORCID: 0000-0002-5292-2088(郑锐)

 

关键词: 骨科材料, 改良型富血小板纤维蛋白, β-磷酸三钙, 临界性骨缺损, 骨再生, 生物材料

Abstract:

BACKGROUND: It has been proved that various bone substitute materials combined with blood derivatives contribute to osteogenesis. At present, no relevant reports have been reported on the combination of β-tricalcium phosphate (β-TCP) combined with advanced platelet-rich fibrin (A-PRF) in the repair of bone defects.

OBJECTIVE: To observe the characteristics and effects of β-TCP combined with A-PRF for repair of bone defects.
METHODS: Thirty-nine Japanese big ear rabbits (provided by the Chengdu Dashuo Experimental Animal Center in China) were randomly divided into A-PRF group (n=12), β-TCP group (n=12), composite group (n=12), and blank control group (n=3). A 6.0 mm×8.0 mm cylindrical critical bone defect was made on the lateral femoral condyle of each side of hind legs of each rabbit and filled in with different materials in corresponding groups, respectively. No implantation was done in the blank control group. The whole femur of each rat was taken at 1, 2 and 3 months after implantation, and X-ray films were taken as well as receptor activator nuclear factor kappa B ligand and osteoprotegerin immunohistochemical analysis.

RESULTS AND CONCLUSION: (1) Over time, X-ray films showed high-density shadow in the bone defect area in the A-PRF group, and there was a centripetal growth trend from the edge of bone defect to the center, while in the β-TCP group and composite group, there was a centripetal decrease trend from the edge to the center of the bone defect, and finally the density was close to natural bone or consistent to the surrounding bone tissues. (2) Receptor activator nuclear factor kappa B ligand and osteoprotegerin immunohistochemical analysis showed a positive expression in all the groups. The order of the mean absorbance value in the four groups was as follows: the composite group > β-TCP group > A-PRF group > blank group, all of which were statistically significant (P < 0.05). To conclude, β-TCP combined with A-PRF has a better osteogenic effect than individual use.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Calcium Phosphates, Bone Regeneration, Osteoprotegerin, Tissue Engineering

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