中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 227-230.doi: 10.3969/j.issn.1673-8225.2012.02.008

• 骨组织构建 • 上一篇    下一篇

骨关节炎兔软骨细胞凋亡与八味柔肝散的抑制效应

刘 淼，陈世荣，刘 伟，徐西东，斯海波   

  1. 重庆医科大学第二附属医院，重庆市400010
  • 收稿日期:2011-10-19 修回日期:2011-12-07 出版日期:2012-01-08 发布日期:2012-01-08
  • 通讯作者: 陈世荣，副教授，骨科主任，重庆医科大学第二附属医院，重庆市 400010 chensr128@126.com
  • 作者简介:刘淼★，男，1985年生，河南省南阳市人，汉族，重庆医科大学第二附属医院在读硕士。 blue_ivyer@sina.com
  • 基金资助:

    2010年重庆市卫生局中医药科研计划项目，编号(2010-2-37)，项目名称：《八味柔肝散对兔骨关节炎软骨损伤修复的实验研究》。

Inhibitory effects of Bawei Rougan powder on chondrocytes apoptosis in rabbits withosteoarthritis

Liu Miao, Chen Shi-rong, Liu Wei, Xu Xi-dong, Si Hai-bo   

  1. Second AffiliatedHospital, ChongqingMedical UniversityChongqing 400010,China
  • Received:2011-10-19 Revised:2011-12-07 Online:2012-01-08 Published:2012-01-08
  • Contact: Chen Shi-rong,Associate professor,Second AffiliatedHospital, ChongqingMedical UniversityChongqing 400010,China chensr128@126.com
  • About author:Liu Miao★, Studyingfor master’s degree,Second AffiliatedHospital, ChongqingMedical UniversityChongqing 400010,China blue_ivyer@sina.com
  • Supported by:
    Traditional ChineseMedicine ResearchProgram of SecondAffiliated HospitalChongqing MunicipalHealth Bureau in2010, No. 2010-2-37*

PDF

489

被引次数

18

摘要：

背景：传统中药对骨关节炎具有镇痛等治疗作用，但对关节软骨是否有保护作用及其通过何种机制起作用少有报道。
目的：观察八味柔肝散对兔骨关节炎软骨细胞凋亡的影响。
方法：将新西兰大白兔随机分为正常组、模型组和治疗组，模型组和治疗组行Hurth 法骨关节炎造模。建模后1 周，正常组和模型组每天生理盐水灌胃，治疗组行八味柔肝散水提液灌胃。建模后8周行组织学观察Mankin's评分，硝酸还原酶法测定关节液一氧化氮、诱导型一氧化氮合酶活性，原位末端转移酶标记技术检测软骨细胞的凋亡指数。
结果与结论：结果显示，治疗组关节软骨Mankin's评分，关节液一氧化氮、诱导型一氧化氮合酶活性，关节软骨细胞凋亡指数均低于模型组(P < 0.05)。证实八味柔肝散对兔骨关节炎软骨细胞凋亡有抑制作用。

关键词: 八味柔肝散, 骨关节炎, 一氧化氮, 诱导型一氧化氮合酶, 软骨, 凋亡, 组织构建

Abstract:

BACKGROUND: Traditional Chinese Medicine (TCM) has analgesic effects on osteoarthritis, but whether TCM can protectarticular cartilage and through which mechanism are rarely reported.
OBJECTIVE: To observe the effects of Bawei Rougan powder on chondrocytes apoptosis in rabbits with osteoarthritis.
METHODS: New Zealand adult white rabbits were randomly divided into three groups: normal, model, and treatment groups.Osteoarthritis models were established in model and treatment groups according to Hurth technique. At 1 week after modeling,rabbits in the normal and model groups were treated with saline solution, and the treatment group was treated with the powderextract. At 8 weeks after modeling, characteristics of cartilage histological were observed by Mankin method. Activity of nitricoxide (NO) and inducible nitric oxide synthase (iNOS) in synovial fluids were measured by nitrate reductase assay, and apoptosisindex of chondrocytes in all groups were measured by TUNEL ( TdT-mediated biotinylated-dUTP nick end labling method).
RESULTS AND CONCLUSION: Compared with the model group, Mankin's score, activity of NO and iNOS and apoptosis indexof chondrocytes were decreased in the treatment group (P < 0.05). It is confirmed that Bawei Rougan powder can suppress theapoptosis of chondrocytes in rabbits with osteoarthritis．

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