中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 223-226.doi: 10.3969/j.issn.1673-8225.2012.02.007

• 骨髓干细胞 • 上一篇    下一篇

胰岛素样生长因子1及血小板源性生长因子对人退变髓核细胞生物学活性的影响

林  旺，刘寿坤，王盈盈，郭卫中，林成寿，王  旭，苏  郁
  

  1. 福建医科大学附属宁德市闽东医院，福建省福安市  355002
  • 收稿日期:2011-08-19 修回日期:2011-12-08 出版日期:2012-01-08 发布日期:2012-01-08
  • 通讯作者: 刘寿坤，主任医师，福建医科大学附属宁德市闽东医院，福建省福安市355002 yaodao.81@163.com
  • 作者简介:林旺★，男，1978年生，福建省福安市人，汉族，硕士， 2009年福建医科大学毕业，医师，主要从事骨科医疗工作。 yaodao.81@163.com

Effect of insulin-like growth factor-1 and platelet-derived growth factor on the biologicalpotential of human degenerative nucleus pulposus cells

Lin Wang, Liu Shou-kun, Wang Ying-ying, Guo Wei-zhong, Lin Cheng-shou, Wang Xu, Su Yu   

  1. Mindong HospitalAffiliated to FujianMedical University,Fuan 355002,Fujian Province,China

     
  • Received:2011-08-19 Revised:2011-12-08 Online:2012-01-08 Published:2012-01-08
  • Contact: Liu Shou-kun, Chiefphysician, MindongHospital Affiliated toFujian MedicalUniversity, Fuan355002, FujianProvince, China yaodao.81@163.com
  • About author:Lin Wang★, Master,Physician, MindongHospital Affiliated toFujian MedicalUniversity, Fuan355002, FujianProvince, China yaodao.81@163.com

PDF

399

被引次数

8

摘要：

背景：有研究表明胰岛素样生长因子1和血小板源性生长因子可抑制人椎间盘细胞凋亡。
目的：观察在体外培养条件下胰岛素样生长因子1、血小板源性生长因子对人退变髓核细胞生物学活性的影响。
方法：体外单层培养人退变髓核细胞，通过免疫组织化学鉴定细胞。对传3代人退变髓核细胞采用分别不同生长因子干预，实验分4组：胰岛素样生长因子1组，血小板源性生长因子组，胰岛素样生长因子1+血小板源性生长因子组及对照组。
结果与结论：胰岛素样生长因子1、血小板源性生长因子均可促进细胞增殖，促进细胞合成Ⅱ型胶原和聚集蛋白聚糖，其中胰岛素样生长因子1促Ⅱ型胶原合成作用强于血小板源性生长因子(P < 0.05)，血小板源性生长因子促蛋白聚糖合成作用强于胰岛素样生长因子1(P < 0.05)。胰岛素样生长因子1促进细胞合成Ⅰ型胶原，血小板源性生长因子抑制细胞合成Ⅰ型胶原。结果证实，胰岛素样生长因子1、血小板源性生长因子均可通过促进细胞增殖、促进细胞合成Ⅱ型胶原和聚集蛋白聚糖，从而提高人退变髓核细胞的生物学活性。

关键词: 胰岛素样生长因子1, 血小板源性生长因子, 髓核细胞, 退变, 组织工程

Abstract:

BACKGROUND: Previous studies showed that insulin-like growth factor-1 (IGF-1) and platelet-derived growth factor (PDGF) cansuppress apoptosis of human intervertebral cells.
OBJECTIVE: To observe the effects of IGF-1 and PDGF on the biological potential of the human degenerative NP cells in vitro.
METHODS: Human degenerative nucleus pulposus cells were cultured in monolayer in vitro and identified byimmunohistochemical staining. The cells of passage 3 were treated separately with different growth factors and then randomlydivided into 4 groups: IGF-1 group, PDGF group, IGF-1+PDGF group and control group.
RESULTS AND CONCLUSION: IGF-1 and PDGF could stimulate cell proliferation and synthesis of collagen type Ⅱ andaggrecan, and IGF-1 raised more content of collagen typeⅡ than the PDGF (P < 0.05), but the PDGF raised more content ofaggrecan than the IGF-1 (P < 0.05). IGF-1 stimulated the synthesis of collagen typeⅠ, then PDGF inhibited the synthesis. Boththe IGF-1 and PDGF can stimulate cell proliferation, the synthesis of collagen type Ⅱ and aggrecan, thereby enhance thebiological potential of the human degenerative nucleus pulposus cells.

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