中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (30): 4496-4502.doi: 10.3969/j.issn.2095-4344.2016.30.013

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

 成熟瘢痕组织与正常皮肤脱细胞真皮基质的生物学性能比较

王杨杨1，李小静2，卜寿山3   

  1. 1安徽省淮北市人民医院，安徽省淮北市  235000；2安徽医科大学第一附属医院整形外科，安徽省合肥市 230032；3江苏省人民医院，江苏省南京市 210029
  • 收稿日期:2016-05-04 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: 李小静，硕士，教授，主任医师，安徽医科大学第一附属医院整形外科，安徽省合肥市 230032
  • 作者简介:王杨杨，女，1985年生，江苏省泗洪县人，医师，主要从事整形外科研究。
  • 基金资助:

    江苏省自然科学基金面上项目(BK2011844)

Biological properties of acellular dermal matrix derived from mature scar tissue versus normal skin

Wang Yang-yang1, Li Xiao-jing2, Bu Shou-shan3   

  1. 1Huaibei People’s Hospital of Anhui, Huaibei 235000, Anhui Province, China; 2Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China; 3Jiangsu Provincial People’s Hospital, Nanjing 210029, Jiangsu Province, China
  • Received:2016-05-04 Online:2016-07-15 Published:2016-07-15
  • Contact: Li Xiao-jing, Master, Professor, Chief physician, Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China
  • About author:Wang Yang-yang, Physician, Huaibei People’s Hospital of Anhui, Huaibei 235000, Anhui Province, China
  • Supported by:

    the Natural Science Foundation of Jiangsu Province, No. BK2011844

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摘要：

 文章快速阅读：

 

文题释义：
脱细胞真皮基质：是一种真皮代替物，利用物理或化学方法将异体或异种皮肤中的细胞成分去除，仅保留含胶原网架的细胞外基质，将其在体外培养系统作为支架，能调节成纤维细胞，下调其分泌细胞外基质包括胶原的过度表达。
脱细胞真皮基质的制作：目前主要有3类方法，第1类是酶消化法，目前应用较多；第2类是高渗盐去污剂处理法；第3类是平衡盐溶液法，这种方法制备过程比较复杂，目前应用较少。Takami等用胰蛋白酶和乙二胺四乙酸或DispaseⅡ消化皮肤，再以Triton X-100浸泡，可完全除去真皮内的细胞，并且无免疫原性。实验中以DispaseⅡ/Triton X-100方法制备人正常皮肤和人成熟瘢痕组织脱细胞真皮基质。


背景：临床上大面积瘢痕患者需要进行多次手术切除瘢痕，这种切除的瘢痕组织是否可再利用成为研究的重点。
目的：比较成熟瘢痕组织与正常皮肤脱细胞真皮基质的组织结构、生物力学性能及生物相容性等指标。
方法：①取人成熟瘢痕组织和瘢痕周围正常皮肤，分别制备脱细胞真皮基质，进行组织学及扫描电镜观察，利用万能拉力试验机进行生物力学实验；②将成熟瘢痕组织脱细胞真皮基质与正常皮肤脱细胞真皮基质分别与成纤维细胞共培养10 d，绘制细胞生长曲线；③将成熟瘢痕组织脱细胞真皮基质与正常皮肤脱细胞真皮基质分别置入SD大鼠背部皮下，置入后4，8，12周进行组织学观察。
结果与结论：①两组脱细胞真皮基质内无任何细胞成分，可见许多空隙，成熟瘢痕组织脱细胞真皮基质胶原纤维较正常皮肤松散，排列稍显紊乱；成熟瘢痕组织脱细胞真皮基质的生物力学性能与正常皮肤脱细胞真皮基质相近，有足够的弹性和强度；②两种脱细胞真皮基质上的细胞生长状态无明显差异，生长曲线基本一致；③置入4周时，成熟瘢痕组织脱细胞真皮基质组可观察到较多炎细胞浸润，正常皮肤脱细胞真皮基质组仅见少量炎症细胞浸润，随时间的延长，炎性反应逐渐消失，两组胶原纤维排列规整，植入物内均有小血管长入；④结果表明，瘢痕组织脱细胞真皮基质的组织结构、生物力学性能及生物相容性均十分接近人体正常皮肤。

ORCID: 0000-0002-1194-0984(李小静)

关键词: 生物材料, 材料相容性, 脱细胞真皮基质, 皮肤, 成熟瘢痕组织, 组织结构, 生物力学, 生物相容性, 江苏省自然科学基金

Abstract:

BACKGROUND: It is necessary to carry out multiple operations to remove the scar in patients with large area of scar, and whether the scar tissue can be recycled has become the focus of the study.
OBJECTIVE: To compare the tissue structure, biomechanical properties and biocompatibility of the acellular dermal matrix of mature scar tissue and normal skin.
METHODS: The acellular dermal matrix was prepared from the human mature scar tissue and the normal skin around the scar. Subsequently, histological and scanning electron microscope observations were performed, and biomechanical properties were detected using universal tensile testing machine. Then, the acellular dermal matrix from mature scar tissue and normal skin was co-cultured with fibroblasts for 10 days, respectively, and the cell growth curve was drawn. Additionally, the acellular dermal matrix from mature scar tissue and normal skin was subcutaneously implanted into the dorsal tissue of Sprague-Dawley rats, respectively and histological observation was conducted at 4, 8 and 12 weeks after implantation.
RESULTS AND CONCLUSION: There were many gaps but no cellular components in the acellular dermal matrix, in both two groups. Collagen fibers of the acellular dermal matrix derived from mature scar were looser than that of the normal skin, and arranged slightly irregularly; the biomechanical properties of the acellular dermal matrix derived from mature scar were similar to that of the normal skin, which exhibited appropriate flexibility and strength. There was no significant difference in the growth state of the two kinds of acellular dermal matrix, and the growth curve was basically consistent. After 4 weeks of implantation, more inflammatory cells infiltration could be found in the mature scar group, and in contrast, only a few inflammatory cells infiltration appeared in the normal skin group, These inflammatory reactions disappeared with time in both two groups. Besides, collagen fibers arranged in neat, and small vessels grew into the implants in both two groups. In conclusion, the tissue structure, biomechanical properties and biocompatibility of the acellular dermal matrix derived from scar tissue are almost consistent with those of the human normal skin.

Key words: Cicatrix, Skin, Biomechanics, Tissue Engineering

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