中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (18): 3295-3302.doi: 10.3969/j.issn.2095-4344.2013.18.010

• 移植与人工器官 transplantation and artificial organs • 上一篇    下一篇

中国人肝细胞系1运用于生物人工肝的生物代谢功能

饶小惠,简国登,张  志,汪  艳,潘明新,高  毅   

  1. 南方医科大学珠江医院肝胆二科,广东省广州市  510280
  • 收稿日期:2013-02-12 修回日期:2013-03-18 出版日期:2013-04-30 发布日期:2013-04-30
  • 通讯作者: 潘明新,博士,博士生导师,主任医师,教授,南方医科大学珠江医院肝胆二科,广东省广州市 510280 pmxwxy@sohu.com 高毅,博士,博士生导师,主任医师,教授,南方医科大学珠江医院肝胆二科,广东省广州市 510280 gaoyi6164@163.com
  • 作者简介:饶小惠★,男,1987年生,广东省惠州市人,汉族,2013年南方医科大学毕业,硕士,主要从事组织工程、生物人工肝方面的研究。 oulingjv@163.com
  • 基金资助:

    国家高技术发展计划(863计划)(2006AA 02A141),课题名称:人源细胞混合型生物人工肝的研制与开发。

Biological metabolic function of the human liver cell line-1 used in bioartificial liver

Rao Xiao-hui, Jian Guo-deng, Zhang Zhi, Wang Yan, Pan Ming-xin, Gao Yi   

  1. Second Department of Hepatology, Zhujang Hospital of Southern Medical University, Guangzhou  510280, Guangdong Province, China
  • Received:2013-02-12 Revised:2013-03-18 Online:2013-04-30 Published:2013-04-30
  • Contact: Pan Ming-xin, Doctor, Doctoral supervisor, Chief physician, Professor, Second Department of Hepatology, Zhujang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China pmxwxy@sohu.com Gao Yi, Doctor, Doctoral supervisor, Chief physician, Professor, Second Department of Hepatology, Zhujang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China gaoyi6164@163.com
  • About author:Rao Xiao-hui★, Master, Second Department of Hepatology, Zhujang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China oulingjv@163.com
  • Supported by:

    National High Technology Development Planning Project of China(863 Planning), No. 2006AA02A141

摘要:

背景:前期研究发现,中国人肝细胞系1细胞分化程度高且生物代谢功能良好, 并且中国人肝细胞系1细胞组织学上来源于正常肝组织,较其他来源于肿瘤源性的肝细胞系更为安全。
目的:探讨中国人肝细胞系中国人肝细胞系1细胞在混合型生物人工肝中的生物代谢功能。
方法:15只食蟹猴随机分成对照组(n=5)和治疗组(n=10),均建立急性肝功能衰竭模型,治疗组接受以全接触灌流型生物反应器接种微载体微重力中国人肝细胞系1细胞建立的人源细胞混合型生物人工肝进行治疗。
结果与结论:急性肝功能衰竭食蟹猴血清谷氨酸转氨酶、总胆红素、总胆汁酸、尿素氮、肌酐、血氨均明显上升,而白蛋白、Fischer指数则显著下降;人源细胞混合型生物人工肝治疗后,急性肝功能衰竭食蟹猴血清谷氨酸转氨酶、总胆红素、总胆汁酸、尿素氮、肌酐、血氨和白蛋白均恢复。提示中国人肝细胞系1细胞在混合型生物人工肝中生物代谢功能良好,表现出良好的肝特异性生物合成及生物代谢功能。

关键词: 器官移植, 移植与人工器官, 人肝细胞, 人工器官, 混合型生物人工肝, 肝, 肝特异性生物合成, 食蟹猴, 863项目

Abstract:

BACKGROUND: Our preliminary study found that Chinese human liver cell line-1 has high differentiation ability and good biological metabolic functions, and the human liver cell lines are separated from normal liver tissue on a cell histology which are safer than the tumor-derived liver cell line.
OBJECTIVE: To investigate the biological metabolic functions of Chinese human liver cell line-1 cells in a hybrid bioartificial liver.
METHODS: Fifteen cynomolgus monkeys were randomly divided into the control group (n=5) and the treatment group (n=10), and all the monkeys were used to establish the acute liver failure models. The models in the treatment group were implanted with Chinese human liver cell line-1 established hybrid bioartificial liver through the contact perfusion bioreactor inoculation under micro carrier microgravity.
RESULTS AND CONCLUSION: The serum alanine aminotransferase, total bilirubin, total bile acid, urea nitrogen, creatinine and blood ammonia levels of the cynomolgus monkeys with acute liver failure were increased, while the albumin level and Fischer index were decreased; after treated with hybrid bioartificial liver, serum alanine aminotransferase, total bilirubin, total bile acid, urea nitrogen, creatinine and blood ammonia levels of the cynomolgus monkeys with acute liver failure were recovered to the normal levels. It suggests that Chinese human liver cell line-1 cells in hybrid bioartificial liver have good biological metabolic function with good liver-specific biosynthesis and biological metabolic functions.

Key words: organ transplantation, transplantation and artificial organs, human hepatocytes, artificial organs, hybrid bioartificial liver, liver, liver-specific biosynthesis, cynomolgus monkeys, National 863 Project of China

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