中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (18): 4782-4790.doi: 10.12307/2026.756

• 组织工程相关大数据分析 Big data analysis in tissue engineering • 上一篇    下一篇

炎症因子、白细胞与腰椎间盘突出症的关系

辜  山1，张  龙2，李志刚3   

  1. 1西南医科大学运动与健康促进研究中心，四川省泸州市  646000；2自贡市第一人民医院康复科，四川省自贡市  643000；3西南医科大学体育学院，四川省泸州市  646000


  • 收稿日期:2025-08-18 接受日期:2025-10-30 出版日期:2026-06-28 发布日期:2025-12-12
  • 通讯作者: 李志刚，博士，副教授，硕士研究生导师，西南医科大学体育学院，四川省泸州市 646000
  • 作者简介:辜山，男，1992年生，硕士，讲师，主要从事运动康复、体医融合研究。
  • 基金资助:
    中国攀西康养产业研究中心重点项目(PXKY-ZD-202403)，项目负责人：辜山；西南医科大学高等教育教学改革与研究项目(JG2024154)，项目负责人：辜山；西南医科大学研究生教育教学改革项目(YJG202255)，项目负责人：辜山

Relationship between inflammatory factors, white blood cells, and lumbar disc herniation

Gu Shan1, Zhang Long2, Li Zhigang3   

  1. 1Research Center for Sports and Health Promotion, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Department of Rehabilitation, Zigong First People’s Hospital, Zigong 643000, Sichuan Province, China; 3School of Physical Education, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2025-08-18 Accepted:2025-10-30 Online:2026-06-28 Published:2025-12-12
  • Contact: Li Zhigang, PhD, Associate Professor, Master’s supervisor, School of Physical Education, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Gu Shan, MS, Lecturer, Research Center for Sports and Health Promotion, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:
    Key Project of China Panxi Health Industry Research Center, No. PXKY-ZD-202403 (to GS); Higher Education Teaching Reform and Research Project of Southwest Medical University, No. JG2024154 (to GS); Postgraduate Education and Teaching Reform Project of Southwest Medical University, No. YJG202255 (to GS)

PDF

13

摘要：


文题释义：
腰椎间盘突出症：是指腰椎间盘发生退行性病变后，纤维环部分或全部破裂，髓核单独或者连同纤维环、软骨终板向外突出，刺激或压迫窦椎神经和神经根引起的以腰腿痛为主要症状的一种综合征。
孟德尔随机化：是新兴的因果推断方法，利用遗传变异作为工具变量，可有效规避观察性研究中的混杂偏倚和反向因果干扰。

背景：腰椎间盘突出症是一种临床高发的脊柱疾病，现有研究证据提示细胞因子、白细胞与腰椎间盘突出症发生、发展有重要联系，但具体机制尚不明确。
目的：应用孟德尔随机化分析，探究细胞因子、白细胞与腰椎间盘突出症的因果关系。
方法：选取GWAS Catalog数据库中的91个细胞因子数据和来自血细胞联盟的6种白细胞数据为暴露，最新版R12芬兰数据库中的腰椎间盘突出症数据为结局，采用双向两样本孟德尔随机化和全基因组关联研究共定位分析方法探寻细胞因子、白细胞与腰椎间盘突出症的因果关系。采用Steiger检验、Cochran’s Q检验、MR-Egger截距评估、留一法分析等敏感性检验方法验证结果的准确性。主要使用逆方差加权法进行统计分析。
结果与结论：①嗜碱性粒细胞和嗜酸性粒细胞与腰椎间盘突出症具有因果关系(OR=0.93，95%CI：0.87-0.99；OR=0.94，95%CI：0.88-1.00)；②S100钙结合蛋白A12水平(OR=0.74，95%CI：0.55-1.00)、成纤维细胞生长因子水平(OR=1.03，95%CI：1.00-1.07)、白细胞介素20受体α蛋白水平(OR=1.09，95%CI：1.04-1.15)、白细胞介素6水平(OR=1.07，95%CI：1.00-1.13)、白细胞介素7水平(OR=1.08，95%CI：1.01-1.16)、干细胞因子水平(OR=1.05，95%CI：1.01-1.09)、白细胞介素2水平(OR=0.94，95%CI：0.89-0.99)与腰椎间盘突出症具有因果关系；③共定位分析发现，干细胞因子水平H3+H4=0.80，其中最显著的单核苷酸多态性为rs6073966。结果表明，嗜碱性粒细胞、嗜酸性粒细胞、S100钙结合蛋白A12、成纤维细胞生长因子、白细胞介素20受体α蛋白、白细胞介素2、白细胞介素6、白细胞介素7、干细胞因子对腰椎间盘突出症具有单向因果作用；干细胞因子和腰椎间盘突出症之间可能存在相关通路。

https://orcid.org/0009-0001-0254-8155(李志刚)


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 细胞因子, 炎症, 双向两样本孟德尔随机化, 共定位, Steiger检验, 腰椎间盘突出症

Abstract: BACKGROUND:  Lumbar disc herniation is a clinically prevalent spinal disease. Existing evidence suggests that cytokines and white blood cells are closely associated with the occurrence and progression of lumbar disc herniation, but the specific mechanisms remain unclear.
OBJECTIVE: To explore the causal relationship between cytokines, white blood cells, and lumbar disc herniation using Mendelian randomization analysis.
METHODS: Using 91 cytokine datasets from the GWAS Catalog database and six types of white blood cell data from the Blood Cell Consortium as exposures, along with data on lumbar disc herniation from the latest R12 Finnish database as the outcome, bidirectional two-sample Mendelian randomization and genome-wide association study colocalization analyses were performed to investigate the causal relationships between cytokines, white blood cells, and lumbar disc herniation. Sensitivity tests, including the Steiger test, Cochran’s Q test, MR-Egger intercept assessment, and leave-one-out analysis, were conducted to verify the accuracy of the results. The inverse variance weighting method was primarily used for statistical analysis.
RESULTS AND CONCLUSION: (1) Basophils and eosinophils showed causal relationships with lumbar disc herniation (OR=0.93, 95%CI: 0.87–0.99; OR=0.94, 95%CI: 0.88–1.00). (2) Levels of S100 calcium-binding protein A12 (OR=0.74, 95%CI: 0.55–1.00), fibroblast growth factor (OR=1.03, 95%CI: 1.00–1.07), interleukin-20 receptor α protein (OR=1.09, 95%CI: 1.04–1.15), interleukin-6 (OR=1.07, 95%CI: 1.00–1.13), interleukin-7 (OR=1.08, 95%CI: 1.01–1.16), stem cell factor (OR=1.05, 95%CI: 1.01–1.09), and interleukin-2 (OR=0.94, 95%CI: 0.89–0.99) were causally associated with lumbar disc herniation. (3) In the colocalization analysis, the level of stem cell factor was found to be H3+H4=0.80, with the most significant single nucleotide polymorphism being rs6073966. These findings suggest that basophils, eosinophils, S100 calcium-binding protein A12, fibroblast growth factor, interleukin-20 receptor α protein, interleukin-2, interleukin-6, interleukin-7, and stem cell factor exhibit unidirectional causal effects on lumbar disc herniation. A potential related pathway may exist between stem cell factor and lumbar disc herniation.


Key words: cytokine, inflammation, bidirectional two-sample Mendelian randomization, colocalization, Steiger test, lumbar disc herniation

中图分类号: