中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (29): 4651-4655.doi: 10.3969/j.issn.2095-4344.1806

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

不同保存介质对人少突胶质前体细胞生物学特性的影响

汪兆艳，杨印祥，王  倩，管  倩，栾  佐   

  1. 解放军总医院第六医学中心，北京市  100048
  • 修回日期:2019-05-20 出版日期:2019-10-18 发布日期:2019-10-18
  • 通讯作者: 栾佐，硕士，主任医师，解放军总医院第六医学中心，北京市 100048
  • 作者简介:汪兆艳，女，1981年生，吉林省桦甸市人，汉族，硕士，主要从事干细胞相关研究。
  • 基金资助:

    国家重点研发计划干细胞及转化研究资助(2017YFA0104200)，项目负责人：栾佐；国家自然科学基金(81471486)，项目负责人：栾佐

Effects of different preservation media on the biological properties of human oligodendrocyte precursor cells

Wang Zhaoyan, Yang Yinxiang, Wang Qian, Guan Qian, Luan Zuo   

  1. The Sixth Medical Center of PLA General Hospital, Beijing 100048, China
  • Revised:2019-05-20 Online:2019-10-18 Published:2019-10-18
  • Contact: Luan Zuo, Master, Chief physician, the Sixth Medical Center of PLA General Hospital, Beijing 100048, China
  • About author:Wang Zhaoyan, Master, the Sixth Medical Center of PLA General Hospital, Beijing 100048, China
  • Supported by:

    the National Key Research and Development Program for Stem Cell and Translational Research, No. 2017YFA0104200 (to LZ); the National Natural Science Foundation of China, No. 81471486 (to LZ)

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摘要：

文章快速阅读：

文题释义：
人少突胶质前体细胞：是中枢神经系统髓鞘形成细胞，在体内可以分化为成熟的少突胶质细胞包绕神经元轴突形成髓鞘结构，用于治疗脑白质损伤等脱髓鞘病变。
冷藏保存介质：干细胞制备完成后，移植前用于细胞短期保存的溶液。临床常用的保存介质：含有不同浓度白蛋白的生理盐水、葡糖糖溶液等。

 

摘要
背景：保存介质直接影响干细胞活性以及细胞功能，目前国内外缺乏对人少突胶质前体细胞保存介质方面的研究。
目的：评价临床常用的保存介质对人少突胶质前体细胞活率的影响。
方法：在4 ℃条件下，将处于对数生长期的人少突胶质前体细胞分别在少突细胞完全培养基、含有1%白蛋白的生理盐水、生理盐水中放置24 h，比较不同保存介质中的细胞活性以及继续培养后的细胞增殖能力。
结果与结论：①细胞冷藏24 h进行锥虫蓝染色，少突细胞完全培养基组、含有1%白蛋白的生理盐水组、生理盐水组细胞活率分别为83%，68%和51%，少突细胞完全培养基组细胞活率最高，单纯生理盐水组细胞活率最低；②细胞冷藏后继续培养1代，3种保存介质中少突胶质前体细胞的扩增能力有逐渐降低的趋势，少突细胞完全培养基组细胞增殖能力最高，其次是含有1%白蛋白的生理盐水组，单纯生理盐水中保存的细胞无法继续增殖。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0002-4255-5042(汪兆艳)

关键词: 少突胶质前体细胞, 保存, 保存介质, 细胞活率, 细胞增殖能力, 国家自然科学基金

Abstract:

BACKGROUND: Preservation media exert direct roles in stem cell activity and cell function, and there is still a lack of research on the preservation medium of human oligodendrocyte precursor cells worldwide.
OBJECTIVE: To evaluate the effect of clinically used preservation media on the viability of human oligodendrocyte precursor cells.
METHODS: Human oligodendrocyte precursor cells in logarithmic growth phase were cultured in human oligodendrocyte precursor cell complete medium, normal saline containing 1% albumin or normal saline for 24 hours at 4 °C. We then compared cell viability in different preservation media and cell proliferation ability after continuous culture.
RESULTS AND CONCLUSION: (1) Cell viability was counted by trypan blue staining 24 hours after cell storage. The results showed that the cell viabilities in the oligodenocyte complete medium group, the normal saline group containing 1% albumin group, and the normal saline group were 83%, 68%, and 51%, respectively. The cell viability was highest in the oligodenocyte complete medium group and lowest in the normal saline group. Cryopreserved cells were further cultured, and the cell proliferation ability was declined gradually, which was highest in the oligodenocyte complete medium group, followed by the normal saline containing 1% albumin, whereas the oligodendrocytes preserved in the normal saline could not continue to proliferate.

Key words:  oligodendrocyte precursor cells, preservation, preservation medium, cell viability, cell proliferation ability, National Natural Science Foundation of China

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