中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (25): 3998-4003.doi: 10.3969/j.issn.2095-4344.1771

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

外源性神经生长因子和碱性成纤维细胞生长因子联合应用促进重型脑创伤模型大鼠内源性脑细胞的增殖

王  童,刘  阳,朱业淘
  

  1. 绵阳市第三人民医院(四川省精神卫生中心)神经外科,四川省绵阳市  621000
  • 修回日期:2019-03-13 出版日期:2019-09-08 发布日期:2019-09-08
  • 通讯作者: 刘阳,博士,主任医师,绵阳市第三人民医院(四川省精神卫生中心)神经外科,四川省绵阳市 621000
  • 作者简介:王童,男,1992年生,四川省绵阳市人,汉族,在读硕士,主要从事脑外伤、神经干细胞、脑血管疾病研究。
  • 基金资助:

    四川省卫生和计划生育委员会支持项目(17PJ183),项目负责人:刘阳;四川省医学会支持项目(S16038),项目负责人:刘阳;绵阳市卫生和计划生育委员会支持项目(201615),项目负责人:刘阳

Combined use of exogenous nerve growth factor and basic fibroblast growth factor promotes proliferation of endogenous brain cells in a rat model of severe traumatic brain injury

Wang Tong, Liu Yang, Zhu Yetao
  

  1. Department of Neurosurgery, the Third Hospital of Mianyang/Sichuan Mental Health Center, Mianyang 621000, Sichuan Province, China
  • Revised:2019-03-13 Online:2019-09-08 Published:2019-09-08
  • Contact: Liu Yang, MD, Chief physician, Department of Neurosurgery, the Third Hospital of Mianyang/ Sichuan Mental Health Center, Mianyang 621000, Sichuan Province, China
  • About author:Wang Tong, Master candidate, Department of Neurosurgery, the Third Hospital of Mianyang/ Sichuan Mental Health Center, Mianyang 621000, Sichuan Province, China
  • Supported by:

    Sichuan Provincial Health and Family Planning Commission Support Project, No. 17PJ183 (to LY); Sichuan Medical Association Support Project, No. S16038 (to LY); Mianyang Municipal Health and Family Planning Commission Support Project, No. 201615 (to LY)

摘要:

文章快速阅读:

文题释义:
神经营养因子:
是一类有神经营养活性,与神经细胞生长、存活相关的细胞因子的统称,具有增强神经元分化、诱导神经干细胞增殖、增强突触形成、防止神经细胞凋亡等特性,从而发挥支持神经元生长发育和维持神经网络完整性的功能。
神经干细胞:存在于哺乳动物的海马齿状回及室管膜下区,在哺乳动物的一生中都具有从神经干细胞分化为神经元的功能。

 

摘要
背景:
研究证明神经生长因子、碱性成纤维细胞生长因子能够明显在体外促进神经干细胞的自我更新及分化,二者联合应用对成年大鼠脑创伤后内源性脑细胞的影响研究甚少。
目的:探索外源性神经生长因子和碱性成纤维细胞生长因子对重型脑创伤大鼠内源性大脑组织细胞的影响。
方法:以改良Feeney氏法对48只SD大鼠进行脑创伤造模,造模后随机将48只大鼠分为神经生长因子组、碱性成纤维细胞生长因子组、联合组、对照组。造模后24 h,各组分别在脑室内注入对应的神经营养因子,对照组注射生理盐水。采用行为学实验观察肢体恢复情况,免疫组化法比较各组大鼠脑内BrdU阳性细胞的表达。
结果与结论:①从造模第5天开始神经生长因子组、碱性成纤维细胞生长因子组、联合组行为学实验评分均小于对照组(P < 0.05),且联合组评分低于神经生长因子组、碱性成纤维细胞生长因子组(P < 0.05);②神经生长因子组、碱性成纤维细胞生长因子组、联合组BrdU阳性细胞数量明显多于对照组,差异有显著性意义(P < 0.05),同时联合组BrdU阳性细胞明显多于神经生长因子组、碱性成纤维细胞生长因子组(P < 0.05);③结果表明,外源性神经生长因子和碱性成纤维细胞生长因子可加快脑创伤大鼠肢体功能恢复和促进大脑组织细胞的增殖,且神经生长因子和碱性成纤维细胞生长因子的联合使用能取得更加显著的效果。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

关键词: 脑创伤, 神经干细胞, 神经生长因子, 碱性成纤维细胞生长因子, BrdU阳性细胞, 行为学实验

Abstract:

BACKGROUND: Either nerve growth factor or basic fibroblast growth factor can promote the self-renewal and differentiation of neural stem cells in vitro. Little has been reported on the effect of the combination of these two factors on endogenous brain cells after traumatic brain injury in adult rats.
OBJECTIVE: To explore the effects of exogenous nerve growth factor and basic fibroblast growth factor on the proliferation of endogenous brain cells in the rats with severe traumatic brain injury.
METHODS: Animal models of traumatic brain injury were made in 48 adult Sprague-Dawley rats using modified Feeney’s method, and then randomized into 4 groups: nerve growth factor, basic fibroblast growth factor, combination (injection of nerve growth factor and basic fibroblast growth factor), and control (normal saline injection) groups. Intraventricular injection of different factors was performed correspondingly at 24 hours after modeling. The restoration of limb function was assessed through behavior observation. BrdU-positive cells in the brain were counted and compared among four groups using immunohistochemical method.
RESULTS AND CONCLUSION: (1) Initially from the 5th day after modeling, the behavioral test scores in the three treatment groups were significantly lower than those in the control group (P < 0.05), and the behavioral test scores in the combination group were significantly lower than those in the nerve growth factor and basic fibroblast growth factor groups (P < 0.05). (2) There were more BrdU-labeled cells in the three treatment groups than the control group (P < 0.05). The number of BrdU-positive cells in the combination group was significantly higher than that in the nerve growth factor and basic fibroblast growth factor groups (P < 0.05). In summary, the proliferation of brain cells and restoration of limb function after traumatic brain injury can be promoted by nerve growth factor or basic fibroblast growth factor. Morever, this effect can be considerably improved by the combination of nerve growth factor and basic fibroblast growth factor.

Key words: traumatic brain injury, neural stem cells, nerve growth factor, basic fibroblast growth factor, BrdU-positive cells, behavioral tests

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