中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (17): 2746-2752.doi: 10.3969/j.issn.2095-4344.2017.17.020

• 干细胞综述 stem cell review • 上一篇    下一篇

骨髓间充质干细胞的衰老特征及其与治疗的相关性

陈裕浩1,2,朱向情1,潘兴华1   

  1. 1解放军成都军区昆明总医院细胞生物治疗中心,干细胞与免疫细胞生物医药技术国家地方联合工程实验室,云南省干细胞工程实验室,昆明市干细胞与再生医学重点实验室,云南省昆明市  650032;2贵州医科大学,贵州省贵阳市  550025
  • 修回日期:2017-01-20 出版日期:2017-06-18 发布日期:2017-06-29
  • 通讯作者: 潘兴华,博士后,主任医师,硕士生导师,解放军成都军区昆明总医院细胞生物治疗中心,细胞生物医药技术国家地方联合工程实验室,云南省干细胞工程实验室,昆明市干细胞与再生医学重点实验室,云南省昆明市 650032
  • 作者简介:陈裕浩,男,1991年生,福建省福州市人,汉族,贵州医科大学在读硕士,主要从事骨髓间充质干细胞研究。
  • 基金资助:

    国家支持项目(2014BIO1B01);昆明市重点项目(2015-1-S-00973)

Bone marrow mesenchymal stem cells: aging features and relevant treatments

Chen Yu-hao1, 2, Zhu Xiang-qing1, Pan Xing-hua1   

  1. 1Cell Biological Therapy Center of Kunming General Hospital of Chengdu Military Area of Chinese PLA, National and Regional Integrated Engineering Laboratory of Stem Cells and Immunocyte Biological Technology, Stem Cell Engineering Laboratory of Yunnan Province, Key Laboratory of Stem Cells and Regenerative Medicine of Kunming City, Kunming 650032, Yunnan Province, China; 2Guizhou Medical University, Guiyang 550025, Guizhou Province, China
  • Revised:2017-01-20 Online:2017-06-18 Published:2017-06-29
  • Contact: Pan Xing-hua, M.D., Chief physician, Master’s supervisor, Cell Biological Therapy Center of Kunming General Hospital of Chengdu Military Area of Chinese PLA, National and Regional Integrated Engineering Laboratory of Stem Cells and Immunocyte Biological Technology, Stem Cell Engineering Laboratory of Yunnan Province, Key Laboratory of Stem Cells and Regenerative Medicine of Kunming City, Kunming 650032, Yunnan Province, China
  • About author:Chen Yu-hao, Studying for master’s degree, Cell Biological Therapy Center of Kunming General Hospital of Chengdu Military Area of Chinese PLA, National and Regional Integrated Engineering Laboratory of Stem Cells and Immunocyte Biological Technology, Stem Cell Engineering Laboratory of Yunnan Province, Key Laboratory of Stem Cells and Regenerative Medicine of Kunming City, Kunming 650032, Yunnan Province, China
  • Supported by:

    the National Support Project of China, No. 2014BIO1B01; the Major Project of Kunming City, No. 2015-1-S-00973

摘要:

文章快速阅读:

文题释义:
骨髓间充质干细胞的衰老:
是一种极其复杂的生理过程,易受微环境的影响。有实验表明,老年小鼠的内环境能够抑制成体干细胞的功能。衰老多源于骨髓间充质干细胞的不断增殖,在细胞增殖的过程中易出现DNA损伤,当DNA损伤积累到一定程度时,即可能导致细胞周期调控发生异常,甚至出现恶变。
延缓骨髓间充质干细胞衰老的途径:目前研究可通过调节端粒酶活性,干预氧化应激或微环境中的Wnt/β-catenin等信号通路,应用p16、p53等基因表达或组蛋白乙酰转移酶、溶血磷脂酸等药物,以及通过机械力、能量限制等非药物手段来延缓或防止骨髓间充质干细胞的衰老,提高其临床疗效。

 

摘要
背景:
骨髓间充质干细胞可能对年龄相关疾病敏感,是影响退行性疾病骨重建的重要因素之一。
目的:综述骨髓间充质干细胞的衰老特征及治疗进展。
方法:检索PubMed数据库2006年6月至2016年6月关于骨髓间充质干细胞衰老及治疗的相关文章,以“MSCs,aging”为关键词进行检索,排除陈旧性和无关研究,最终选择关于骨髓间充质干细胞衰老及治疗的61篇文献,并参考《哈德兹老年医学》进行综述。
结果与结论:骨髓来源间充质干细胞可支持免疫和造血系统中的造血祖细胞生长,促进骨髓组织修复,影响骨的生长和重建,其也会随着年龄及其他因素发生老化,进而影响其功能。衰老的骨髓间充质干细胞表现出端粒缩短、增殖和分化能力减弱、氧化应激水平增高等特点。目前研究可通过调节端粒酶活性,干预氧化应激或微环境中的Wnt/β-catenin 等信号通路,应用p16、p53等基因表达或组蛋白乙酰转移酶、溶血磷脂酸等药物,以及通过机械力、能量限制等非药物手段来延缓或防止骨髓间充质干细胞的衰老,提高其临床疗效,但更确切的机制尚待进一步研究。

 

 

ORCID: 0000-0002-2874-6053(陈裕浩)

关键词: 干细胞, 骨髓干细胞, 骨髓间充质干细胞, 衰老, 抗衰老, 治疗

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells may be sensitive to age-related diseases, which is a contributory factor of bone remodeling after degenerative diseases.
OBJECTIVE: To summarize the features of aging and progress in treatments related to cell aging.
METHODS: We retrieved PubMed database for articles concerning aging of bone marrow mesenchymal stem cells and its treatments published from June 2006 to June 2016. The key words were “MSCs, aging”. Finally, 61 relevant articles were selected and reviewed in reference with Hazzard’s Geriatric Medicine and Gerontology.
RESULTS AND CONCLUSION: Bone marrow derived mesenchymal stem cells can support the growth of hematopoietic progenitor cells in the immune and hematopoietic system, promote repair of the bone marrow, and influence bone growth and remodeling. However, cell aging occurs with age and other factors will affect cell function. Aging bone marrow mesenchymal stem cells show shortened telomere, reduced proliferation and differentiation ability and increased oxidative stress level. Current evidence has confirmed that cell aging can be delayed or prevented by adjustment of age-related telomerase activities, oxidative stress intervention, Wnt/beta-catenin signaling pathway in the microenvironment, regulation of p16, p53 gene expression or drugs such as histone acetyltransferase and lysophosphatidic acid, thereby to improve the clinical curative effect of bone marrow mesenchymal stem cells. However, the exact mechanism remains to be further studied.

 

 

Key words: Stem Cells, Mesenchymal Stem Cells, Aging, Tissue Engineering

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