中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (43): 6416-6423.doi: 10.3969/j.issn.2095-4344.2016.43.005

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纯钛表面淫羊藿苷/TiO2纳米管复合涂层制备与药物早期释放的分析

王非凡1，宋云嘉1，吴文孟1，2，吕武龙1，李  莺1，李长义1
  

  1. 1天津医科大学口腔医学院，天津市  300070；2越南太平医科大学，越南太平省  33000
  • 收稿日期:2016-07-23 出版日期:2016-10-21 发布日期:2016-10-21
  • 通讯作者: 李莺，博士，副主任医师，天津医科大学口腔医学院，天津市 300070
  • 作者简介:王非凡，女，1991年生，天津市人，汉族，天津医科大学口腔医学院在读硕士，主要从事口腔种植材料研究。
  • 基金资助:

    国家自然科学基金资助项目(31470920，81500886)；天津市自然科学基金资助项目(16JCYBJC28700)

Titanium dioxide nanotube composite coating: preparation and early drug-release

Wang Fei-fan1, Song Yun-jia1, Wu Wen-meng1, 2, Lv Wu-long1, Li Ying1, Li Chang-yi1
  

  1. 1School of Stomatology, Tianjin Medical University, Tianjin 300070, China; 2Taiping Medical University of Vietnam, Taiping 33000, Vietnam
  • Received:2016-07-23 Online:2016-10-21 Published:2016-10-21
  • Contact: Li Ying, M.D., Associate chief physician, School of Stomatology, Tianjin Medical University, Tianjin 300070, China
  • About author:Wang Fei-fan, Studying for master’s degree, School of Stomatology, Tianjin Medical University, Tianjin 300070, China
  • Supported by:
    the National Natural Science Foundation of China, No. 31470920, 81500886; the Natural Science Foundation of Tianjin, No. 16JCYBJC28700

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摘要：

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文题释义：
TiO2纳米管：具有很好的机械、化学性质及生物相容性，不仅可促进成骨细胞和骨髓间充质干细胞的黏附、增殖和骨向分化，还可吸附并容纳药物，是一种优良的局部药物载体。现有研究主要集中于在纳米管表面加载抗菌药物或活性成分形成复合涂层，而纳米管表面加载成骨类药物的报道相对较少。
淫羊藿苷：价格低廉、性质稳定，不但可刺激骨髓间充质干细胞中成骨转化因子的活性，诱导干细胞向成骨细胞转化，还可有效增强成骨功能性标志物的表达：促进骨钙素分泌，提高碱性磷酸酶活性，刺激Ⅰ型胶原和成骨细胞特异性转录因子合成等，进而促进成骨细胞的分化和矿化。

背景：对纯钛进行表面改性，提高钛种植体早期骨结合能力是目前研究的热点。
目的：在TiO2纳米管表面加载淫羊藿苷形成复合涂层，研究其载药量及早期释放规律。
方法：通过阳极氧化在光滑纯钛表面形成TiO2纳米管，用扫描电镜、原子力显微镜、接触角测定仪对光滑纯钛及TiO2纳米管进行表征。通过浸泡法在纯钛及TiO2纳米管表面加载淫羊藿苷，使用高效液相色谱法测定两种材料的早期药物释放量，计算出载药量，绘制累积释放量曲线和累计药物释放量百分比曲线。
结果与结论：①TiO2纳米管径为80 nm，其粗糙度大于纯钛(P < 0.05)，接触角小于纯钛(P < 0.05)；②淫羊藿苷/TiO2纳米管组前14 d的累计药物释放量和前4 h的药物累积释放曲线明显高于淫羊藿苷/纯钛组；与淫羊藿苷/纯钛组相比，淫羊藿苷/TiO2纳米管组累计药物释放量百分比曲线更为平缓，释放时间更长；③结果表明，淫羊藿苷/TiO2纳米管复合涂层可提供较高的药物加载量并具有缓释功能。

关键词: 生物材料, 缓释材料, 纯钛, TiO2纳米管, 淫羊藿苷, 骨结合, 表征, 载药, 浸泡法, 药物释放, 缓释, 国家自然科学基金

Abstract:

BACKGROUND: The surface modification of pure titanium has become a hotspot for research on improving the early implant-osseointegration ability.
OBJECTIVE: To construct the icariin/TiO2 nanotube composite coating, and to explore its drug-loading rate and early drug-release kinetics.
METHODS: Pure titanium was anodized to obtain TiO2 nanotube. Thereafter, the pure titanium and TiO2 nanotube were characterized by scanning electron microscopy, atomic force microscopy and contact angle instrument. Icariin was loaded onto the pure titanium and TiO2 nanotube by immersion method. Afterwards, the early drug-releasing amount of the two materials was measured using high-performance liquid chromatography. Accumulated release and accumulated drug-release percentage curves were drawn.
RESULTS AND CONCLUSION: The diameter of TiO2 nanotube was 80 nm, and the roughness of TiO2 nanotube was significantly higher than that of pure titanium (P < 0.05), but the contact angle was significantly lower than that of pure titanium (P < 0.05). Moreover, compared with the icariin/pure titanium group, the icariin/TiO2 nanotube group had obviously higher accumulated drug-releasing amount at former 14 days and accumulated drug-releasing carve at former 4 hours. The accumulated drug-releasing percentage curve in the icariin/TiO2 nanotube group was flatter than that in the icariin/pure titanium group, suggesting a longer release time. To conclude, the icariin/TiO2 nanotube composite coating has a higher drug, loading and exerts better sustained-release effect.

Key words: Titanium, Nanotubes, Synostosis, Tissue Engineering

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