中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (30): 4820-4826.doi: 10.3969/j.issn.2095-4344.2015.30.013

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

纤维化胶原蛋白海绵的制备及其自组装工艺

赵 颖1，陆金婷2，邓 超2，任伟业2，陈敬华1   

  1. 1江南大学药学院，江苏省无锡市  214122； 
    2无锡贝迪生物工程有限公司，江苏省无锡市  214092
  • 出版日期:2015-07-16 发布日期:2015-07-16
  • 通讯作者: 陈敬华，博士，教授，博士生导师，江南大学药学院，江苏省无锡市 214122
  • 作者简介:赵颖，女，1989年生，山东省枣庄市人，江南大学药学院在读硕士，主要从事生物医用材料研究。
  • 基金资助:

    教育部博士点基金(20110093110008)；江苏省产学研联合创新资金-前瞻性联合研究项目(BY2014023-17)

Preparation and self-assembly process of fibrillar collagen sponges

Zhao Ying1, Lu Jin-ting2, Deng Chao2, Ren Wei-ye2, Chen Jing-hua1   

  1. 1School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, Jiangsu Province, China; 
    2Beidi Biological Engineering Co., Ltd., Wuxi 214092, Jiangsu Province, China
  • Online:2015-07-16 Published:2015-07-16
  • Contact: Chen Jing-hua, M.D., Professor, Doctoral supervisor, School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, Jiangsu Province, China
  • About author:Zhao Ying, Studying for master’s degree, School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, Jiangsu Province, China
  • Supported by:

    the Research Fund for the Doctoral Program of Higher Education of China, No. 20110093110008; Jiangsu Production-Learning-Research Joint Innovation Fund, No. BY2014023-17

PDF

288

被引次数

8

摘要：

背景：传统的胶原蛋白海绵支架降解速度过快，机械强度低，在应用过程中容易塌陷，很难维持其固有形态，不能满足长期执行细胞支架功能的需要，常见的交联方式又存在细胞毒性或胶原蛋白变性等缺陷。

目的：设计一种新的交联工艺，并对工艺条件进行优化，制备出力学性能和耐降解性能良好的胶原蛋白海绵支架材料。

方法：通过体外自组装技术对胶原蛋白进行改性处理，制备具有丝状结构的胶原蛋白纤维，采用单因素分析考察初始胶原蛋白质量浓度、磷酸盐终浓度及pH值对胶原蛋白自组装转化率的影响；根据单因素实验结果，采用正交实验得到最佳胶原蛋白自组装工艺条件。将最佳工艺条件制备的胶原蛋白溶液与未进行改性处理的胶原蛋白溶液冷冻干燥得到胶原蛋白海绵，并进行表征。

结果与结论：胶原蛋白体外自组装的最佳工艺为：初始胶原蛋白质量浓度为2 g/L，pH=8，磷酸盐终浓度为15 mmol/L，此时自组装转化率最大。扫描电镜显示改性处理胶原蛋白海绵具有纤维丝构成的网状结构，其溶胀率、保水率、机械强度均高于未改性处理胶原蛋白海绵(P < 0.05)，克服了未改性处理胶原蛋白海绵降解过快的缺陷。

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 生物材料, 材料相容性, 胶原蛋白海绵, 支架材料, 自组装, 纤维化, 单因素分析, 工艺优化, 机械强度, 耐降解性能

Abstract:

BACKGROUND: An ideal scaffold material needs appropriate degradation rate and certain mechanical properties, but the traditional collagen sponge scaffold has rapid degradation velocity and low mechanical strength, which is easy to collapse and difficult to maintain its natural form. Traditional cross-linking methods also have the problems of cytotoxicity or collagen denaturation, severely limiting the application of collagen.
OBJECTIVE: To design a new cross-linking method for collagen and to optimize the self-assembly process so as to develop a collagen sponge scaffold with good mechanical properties and resistance to degradation.
METHODS: Collagens were modified by self-assembly technology to prepare collagen fibrils which were then freeze-dried into fibrillar collagen sponges. Meanwhile, we optimized the conditions of self-assembly by using orthogonal experiment based on univariate analysis of the effect of initial collagen mass concentration, final phosphate concentration and pH value on the conversion yield of collagen self-assembly.
RESULTS AND CONCLUSION: We optimized the conditions of self-assembly revealed that the optimum 
conditions to prepare collagen fibrils were determined as pH=8.0, initial collagen concentration=2 mg/mL, and final concentration of phosphate=15 mmol/L. The results of scanning electron microscope showed that fibrillar collagen sponges were characterized by refined porous structure which was connected by collagen fibrils. In addition, the fibrillar collagen sponges showed better equilibrium-swelling ratio, water retaining property and mechanical strength compared with unmodified collagen spondages (P < 0.05), to solve the problems in rapid degradation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Collagen, Cross-Linking Reagents

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