中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (1): 107-110.doi: 10.3969/j.issn.1673-8225.2012.01.023

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

中药复方对急性髓系白血病干细胞的影响*★

刘  奎，徐瑞荣，胡述博，王晓玲，崔  兴，王  琰，王敬毅   

  1. 山东中医药大学附属医院血液科，山东省济南市 250011
  • 收稿日期:2011-06-12 修回日期:2011-07-09 出版日期:2012-01-01 发布日期:2012-01-01
  • 作者简介:刘奎★，男，1981年生，山东省新泰市人，汉族，2009年山东中医药大学毕业，硕士，医师，主要从事血液病的临床与实验研究。 mhfxq@163.com
  • 基金资助:

    国家自然科学基金项目(30672746)。

Effects of Chinese herbal compound on acute myeloid leukemia stem cells

Liu Kui, Xu Rui-rong, Hu Shu-bo, Wang Xiao-ling, Cui Xing, Wang Yan, Wang Jing-yi   

  1. Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan  250011, Shandong Province, China
  • Received:2011-06-12 Revised:2011-07-09 Online:2012-01-01 Published:2012-01-01
  • About author:Liu Kui★, Master, Physician, Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, Shandong Province, China mhfxq@163.com
  • Supported by:
    the National Natural Science Foundation of China, No. 30672746*
     

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摘要：

背景：白血病的发生发展、治疗耐药和容易复发的根本原因在于白血病患者体内存在一群白血病干细胞。
目的：观察中药复方对急性髓系白血病干细胞的影响。
方法：30例患者骨髓进行免疫磁珠分选白血病干细胞 CD34+CD38-，将所得每例白血病干细胞细胞混悬液分成2组：对照组不加药物，实验组加入中药复方制剂，终浓度为100 mg/L。
结果与结论：中药复方对于白血病干细胞的抑制率为(36.660±0.018)%，核转录因子κB在22例标本中有表达，阳性率73.33%(22/30)。电泳迁移率检测核转录因子κB 活性，通过凝胶成像系统分析DNA蛋白质结合条带灰度值的比较，实验组核转录因子κB活性低于对照组(P < 0.05)。结果表明，中药复方可干预白血病干细胞的生长状态，可能是通过抑制核转录因子κB活性实现的。
 

关键词: 白血病干细胞, 中药复方, 急性髓系白血病, 核因子κB, 白血病, CD34+, CD38

Abstract:

BACKGROUND: The existence of leukemic stem cells (LSCs) in leukemic patients is the basment how leukemia occurs and develops, drug resistance and recurrence happens.
OBJECTIVE: To investigate the effects of Chinese herbal compound on the LSCs of acute myeloid leukemia (AML) patients.
METHODS: CD34+CD38- LSCs were isolated from the bone marrow of 30 AML patients by immunomagnetic beads. The cells were divided into two groups: Control group were treated without medicine, while experiment group were treated by Chinese herbal compound preparation with terminal concentration of 100 mg/L.
RESULTS AND CONCLUSION: The inhibition rate of Chinese herbal compound to LSCs was (36.660±0.018)%. Nuclear factor κB (NF-κB) expressed in 22 specimens, and the positive rate was 73.33% (22/30). The activity of NF-κB was tested by electrophoretic mobility. DNA-protein band grey values were analyzed by gel imaging system.The activity of NF-κB in the experiment group was decreased compared with that in the control group (P < 0.05) by comparison of the DNA-protein band grey values. The results showed that Chinese herbal compound may interfere in the growth of LSCs by inhibiting the activity of NF-κB.
 

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