中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (1): 103-106.doi: 10.3969/j.issn.1673-8225.2012.01.022

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

不同质量浓度肉苁蓉总苷诱导大鼠骨髓间充质干细胞向神经细胞样细胞的定向分化

董亚莉,李剑锋   

  1. 内蒙古医学院第二附属医院康复科,内蒙古自治区呼和浩特市 010030
  • 收稿日期:2011-07-23 修回日期:2011-08-30 出版日期:2012-01-01 发布日期:2012-01-01
  • 通讯作者: 李剑锋,硕士,内蒙古医学院第二附属医院康复科,内蒙古自治区呼和浩特市 010030 jianfeng0471@163.com
  • 作者简介:董亚莉,女,1962年生,内蒙古自治区包头市人,蒙古族,1987年内蒙古医学院毕业,主任医师,副主任,主要从事骨关节疾病的康复治疗方面的研究。 mile711@yahoo.com.cn

In vitro directional differentiation of bone marrow mesenchymal stem cells into nerve cells induced by cistanche total glycosides

Dong Ya-li, Li Jian-feng   

  1. Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot  010030, Inner Mongolia Autonomous Region, China
  • Received:2011-07-23 Revised:2011-08-30 Online:2012-01-01 Published:2012-01-01
  • Contact: Li Jian-feng, Master, Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, Inner Mongolia Autonomous Region, China jianfeng0471@163.com
  • About author:Dong Ya-li, Chief physician, Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, Inner Mongolia Autonomous Region, China mile711@yahoo.com.cn

摘要:

背景:肉苁蓉及其所含的化学成分有神经保护作用,并能促进神经功能的恢复。
目的:分析肉苁蓉总苷体外诱导骨髓间充质干细胞分化为神经细胞样细胞的最佳剂量。
方法:分离培养Wistar大鼠骨髓间充质干细胞,并利用β-巯基乙醇及不同质量浓度肉苁蓉总苷诱导骨髓间充质干细胞,分为空白对照组、β-巯基乙醇对照组、肉苁蓉总苷100,200,300 μg/L组进行观察。
结果与结论:诱导7 d后,与空白对照组比较,其他各组活细胞数明显增高(P < 0.05);诱导第3天,与空白对照组比较,内苁蓉总苷各剂量组细胞Nestin、NSE阳性表达率明显增高(P < 0.05)。100,200 μg/L质量浓度肉苁蓉总苷可有效促进骨髓间充质干细胞增殖,并能够明显促进其向神经细胞样细胞分化,300 μg/L质量浓度可以促进骨髓间充质干细胞增殖,但效果不及100,200 μg/L组(P < 0.05)。

关键词: 肉苁蓉总苷, 骨髓间充质干细胞, 定向分化, 神经细胞样细胞, 神经系统疾病

Abstract:

BACKGROUND: Cistanche and its chemical composition play a neuroprotective role, and can promote recovery of neurological function.
OBJECTIVE: To analyze the optimal dose of cistanche total glycosides for in vitro induction of bone marrow mesenchymal stem cells differentiating into nerve cells.
METHODS: Bone marrow mesenchymal stem cells of Wistar rats were isolated and then cultured. β-mercaptoethanol and different concentrations of cistanche total glycosides were used to induce bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cells of Wistar rats were divided into blank control group, β-mercaptoethanol control group, cistanche total glycosides groups of 100, 200, 300 μg/L for observation.
RESULTS AND CONCLUSION: After 7 days of induction, the number of living cells increased obviously in other groups, compared with the blank control group (P < 0.05); at the 3rd day of induction, Nestin, NSE-positive expression rate was significantly higher in cistanche total glycosides groups, compared with the blank control group (P < 0.05). 100, 200 μg/L cistanche total glycosides could effectively promote proliferation of bone marrow mesenchymal stem cells, and significantly promote the differentiation into nerve cells. 300 μg/L cistanche total glycosides could promote proliferation of bone marrow mesenchymal stem cells, but the effect was less than 100, 200 μg/L (P < 0.05).

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