中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (53): 9977-9980.doi: 10.3969/j.issn.1673-8225.2011.53.024

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

不同激素剂量和组合对不同品系小鼠超数排卵的影响

邱泽文1，姜午旗2，高丽波1，罗光彬2，陈  军1，詹红微1，高文婷1，王靖宇1   

  1. 1大连医科大学实验动物中心，辽宁省大连市116044
    2沈阳农业大学，辽宁省沈阳市  110000
  • 收稿日期:2011-06-13 修回日期:2011-09-14 出版日期:2011-12-31 发布日期:2011-12-31
  • 通讯作者: 王靖宇，博士，教授，大连医科大学实验动物中心，辽宁省大连市116044
  • 作者简介:邱泽文★，男，1967年生，辽宁省大连市人，汉族，2008年沈阳农业大学毕业，硕士，实验师，主要从事实验动物和动物实验工作。 qiuzewens@163.com

Different combinations and doses of hormone influence the superovulation of different mouse strains

Qiu Ze-wen1, Jiang Wu-qi2, Gao Li-bo1, Luo Guang-bin2, Chen Jun1, Zhan Hong-wei1, Gao Wen-ting1, Wang Jing-yu1   

  1. 1Experimental Animal Center, Dalian Medical University, Dalian  116044, Liaoning Province, China
    2Shengyang Agriculture University, Shengyang  110000, Liaoning Province, China
  • Received:2011-06-13 Revised:2011-09-14 Online:2011-12-31 Published:2011-12-31
  • Contact: Wang Jing-yu, Doctor, Professor, Experimental Animal Center, Dalian Medical University, Dalian 116044, Liaoning Province, China
  • About author:Qiu Ze-wen★, Master, Laboratory technician, Experimental Animal Center, Dalian Medical University, Dalian 116044, Liaoning Province, China qiuzewens@163.com

PDF

840

被引次数

3

摘要：

背景：超数排卵效果受动物品系、营养水平、年龄、发情周期阶段、光照、超排方法、超排所用激素种类和剂量等诸多因素影响，其中激素剂量和动物品种是关键因素。
目的：探讨不同剂量孕马血清促性腺激素(pregnant mare serum gonadotropin，PMSG)和人绒毛膜促性腺激素(human chorionic gonadotropin, HCG)组合，对不同品系小鼠超数排卵效果的影响。
方法：分别用不同剂量的PMSG和HCG对ICR鼠、KM鼠和BALB/c鼠进行超数排卵处理，比较激素处理后各品系小鼠超数排卵的胚胎总数、平均胚胎率、正常胚胎及平均可用胚率。
结果与结论：5 IU PMSG+7 IU HCG剂量组合对ICR鼠和KM鼠超排处理效果较好，BALB/c鼠超排的最适激素剂量为3 IU PMSG+5 IU HCG；使用5 IU PMSG+7 IU HCG剂量组合分别处理3种品系小鼠时，ICR鼠和KM鼠平均胚胎率和平均可用胚率显著高于 BALB/c鼠(P < 0.05)。为获得较多的胚胎进行相关实验，应当选择ICR和KM等小鼠进行超排，特别是选择国际通用的ICR鼠。

关键词: 小鼠, 超数排卵, 胚胎, ICR鼠, KM鼠, BALB/c鼠, 激素

Abstract:

BACKGROUND: Superovulation is affected by many factors, such as animal strain, nutritional level, age, stage of estrous cycle, light, method, hormone kind and dose used in superovulation. Hormone dose and animal strain are key factors among them.
OBJECTIVE: To explore the effects of different dosage combinations of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG) on the superovulation outcome in different mouse strains.
METHODS: Different dosage combinations of PMSG and HCG were used to treat ICR, KM and BALB/c mice for superovulation, respectively. The total number of embryos, the average number of embryos, the number of good embryo and the rate of good embryo were compared among different mouse strains treated with hormone.
RESULTS AND CONCLUSION: Dosage combination of 5 IU PMSG+7 IU HCG was the best hormone combination for ICR and KM mice, while the best hormone combination for BALB/c mice was 3 IU PMSG+5 IU HCG. When these three mouse strains were treated by 5 IU PMSG+7 IU HCG, the average number of embryo and the rate of good embryo of ICR and KM mice were significantly higher than that of the BALB/c mice (P < 0.05). These findings demonstrate that in order to obtain more embryos for relevant experiments, ICR and KM mice and their inbred strains should be used for superovulation, especially the internationally used ICR mice.

中图分类号: