中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2825-2828.doi: 10.3969/j.issn.1673-8225.2011.15.040

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    下一篇

骨钙素基因Hind Ⅲ多态性与福州地区汉族绝经后妇女骨密度的相关性

聂达荣1，王和鸣2，杨连梓1，彭美玉2 ，陈  可3   

  1. 1福建中医药大学附属第二人民医院骨科，福建省福州市 350003
    2福建中医药大学，福建省福州市 350108
    3福建省中医药研究院，福建省福州市 350003
  • 收稿日期:2010-07-12 修回日期:2010-12-12 出版日期:2011-04-09 发布日期:2013-11-06
  • 作者简介:聂达荣★，男，1971年生，汉族，福建省罗源县人，福建中医药大学在读硕士，主治医师，福建中医药大学附属第二人民医院骨科。 944258053@qq.com
  • 基金资助:

    2007年福建省教育厅资助项目(JA07128)，骨钙素基因Hind Ⅲ多态性对绝经后妇女骨密度的影响。

Correlation between Hind Ⅲ polymorphism of osteocalcin gene and bone mineral density in Han Chinese postmenopausal women in Fuzhou area

Nie Da-rong1, Wang He-ming2, Yang Lian-zi1, Peng Mei-yu2, Chen Ke3   

  1. 1Department of Orthopaedics, Second People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou  350003, Fujian Province, China
    2Fujian University of Traditional Chinese Medicine, Fuzhou  350108, Fujian Province, China
    3Fujian Institute of Traditional Chinese Medicine, Fuzhou  350003, Fujian Province, China
  • Received:2010-07-12 Revised:2010-12-12 Online:2011-04-09 Published:2013-11-06
  • About author:Nie Da-rong★, Studying for master’s degree, Attending physician, Department of Orthopaedics, Second People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China 944258053@qq.com
  • Supported by:

    the Education Department of Fujian Province in 2007, No. JA07128*

PDF

422

被引次数

2

摘要：

背景：骨质疏松症是一种多基因遗传病，骨钙素受体基因多态性与骨密度关系存在地域和人群的差异。
目的：观察绝经后妇女骨钙素基因型频率分布及其与骨密度的关系，探讨福州地区汉族绝经后妇女骨质疏松症的遗传易感基因。
方法：用聚合酶链式反应限制性片段长度多态性分析201例汉族绝经后妇女骨钙素基因型，用双能X射线吸收法测定腰椎、股骨颈，大转子和Ward’s三角4个部位骨密度值。
结果与结论：福州地区汉族绝经后妇女骨钙素基因型频率分布符合Hardy-Weinberg定律(χ2=2.29，P > 0.05)，基因多态性分布依次为HH 5%、hh 46%、Hh 49%，与福州、北京、广州、台湾地区骨钙素基因Hind Ⅲ位点多态性分布频率差异无显著性意义(P  > 0.05)。但是与日本人、白种人差异明显(P  < 0.05)。且HH基因型在大转子骨密度明显高于hh型(P < 0.05)，但不同基因型在第2~4腰椎、股骨颈、Ward’s三角区的骨密度差异无显著性意义。提示绝经后妇女骨钙素基因型与大转子骨密度可能存在一定关联。

关键词: 绝经后骨质疏松, 骨密度, 骨钙素, 多态现象, 汉族, 福州

Abstract:

BACKGROUND: Osteoporosis is a polygenic disease. The relationships between osteocalcin (OC) genotypes and bone mineral density (BMD) are different from regions and populations.
OBJECTIVE: To investigate the relationship between OC genotypes and BMD in postmenopausal women, so as to explore the susceptibility gene of female postmenopausal osteoporosis in Fuzhou region.
METHODS: The OC genotype was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The BMD of lumbar spine, femoral neck, trochanter and Ward’s triangle of 201 Han Chinese postmenopausal women were measured by dual energy X-ray absorptiometry. 
RESULTS AND CONCLUSION: The distribution of OC genotypes of Han Chinese postmenopausal women in Fuzhou followed the Hardy-Weinberg equilibrium (X2=2.29, P > 0.05). The frequencies of OC polymorphism were HH genotype 5%, hh 46%, and Hh 49%, which was not significant different from those of Fuzhou, Beijing, Guangzhou and Taiwan (P > 0.05), but obviously different from Japanese and Caucasians (P < 0.05). There was a significant difference in BMD of trochanter between HH and hh genotypes (P < 0.05), but the difference was not significant in vertebrae L2-4, femoral neck and Ward’s triangular area. There is some association between OC genotypes and BMD in postmenopausal women possibly.

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