中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 2083-2086.doi: 10.3969/j.issn.1673-8225.2011.11.045

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

葛根异黄酮联合维生素D防治骨质疏松的协同效应

扶晓明1，周  艳2，李梓民3，贺栋梁4，李东阳4   

  1. 1解放军169医院骨科，湖南省衡阳市  421002
    2长沙医学院衡阳校区预防医学教研室，湖南省衡阳市  421001
    3湖南省疾病预防控制中心毒理科，湖南省长沙市  410005
    4南华大学公共卫生学院营养学教研室，湖南省衡阳市  421001
  • 收稿日期:2010-09-08 修回日期:2010-10-15 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 周艳，硕士，讲师，长沙医学院衡阳校区预防医学教研室，湖南省衡阳市 421001
  • 作者简介:扶晓明★，男，1976年生，湖南省溆浦县人，土家族，2005年南华大学毕业，硕士，主治医师，主要从事骨科临床研究。
  • 基金资助:

    湖南省教育厅科研课题基金项目(09C143)。

Synergistic effect of total isoflavones of pueraria and vitamin D on prevention and treatment of osteoporosis

Fu Xiao-ming1, Zhou Yan2, Li Zi-min3, He Dong-liang4, Li Dong-yang4   

  1. 1Department of Orthopaedics, the169 Hospital of Chinese PLA, Hengyang  421002, Hunan Province, China
    2Department of Preventive Medicine, Hengyang Campus, Changsha College of Medicine, Hengyang  421001, Hunan Province, China
    3Department of Toxicology, Hunan Provincial Center of Disease Prevention and Control, Changsha  410005, Hunan Province, China
    4Department of Nutriology, School of Public Health, Nanhua University, Hengyang  421001, Hunan Province, China
  • Received:2010-09-08 Revised:2010-10-15 Online:2011-03-12 Published:2011-03-12
  • Contact: Zhou Yan, Master, Lecturer, Department of Preventive Medicine, Hengyang Campus, Changsha College of Medicine, Hengyang 421001, Hunan Province, China zhouyan95gb@ yahoo.com.cn
  • About author:Fu Xiao-ming★, Master, Attending physician, Department of Orthopaedics, the 169 Hospital of Chinese PLA, Hengyang 421002, Hunan Province, China
  • Supported by:

    the Science and Technology Foundation of Education Commission of Hunan Province, No. 09C143*

PDF

519

被引次数

11

摘要：

背景: 葛根异黄酮具有类雌二醇结构特征，对去卵巢大鼠的骨质丢失和骨量的减少有一定的抑制作用。但也有报道其对去卵巢大鼠骨质疏松的防治效果较弱且单独使用效果不明显。
目的：观察葛根异黄酮与维生素D联合对去卵巢大鼠骨质疏松的作用。
方法：将81只3月龄雌性SD大鼠随机分为9组: 除假手术组外其他各组均行双侧卵巢摘除造模。低、中、高剂量TIP组，VitD组，低、中、高剂量联合组在去卵巢基础上分别灌胃给予相应剂量的葛根异黄酮和（或）维生素D；模型组和假手术组不给予药物治疗。给药3个月，计算大鼠子宫系数；测定大鼠血清碱性磷酸酶、血钙、血磷、骨钙素及血清雌二醇水平；检测大鼠股骨骨密度。
结果与结论：与模型组比较，中、高剂量TIP组，低、中、高剂量联合组大鼠子宫系数、雌二醇水平明显增高(P < 0.05)，血清碱性磷酸酶、骨钙素水平明显降低(P < 0.05)，股骨中心及远心骨密度均明显增加(P < 0.05)，均以高剂量联合组作用效果最明显。且在子宫系数，雌二醇、血清碱性磷酸酶、骨钙素水平及股骨远心端骨密度方面，葛根异黄酮和维生素D均表现为协同作用。在血钙、血磷方面，葛根异黄酮和(或)维生素D的作用不明显。说明葛根异黄酮和维生素D联合使用对去卵巢大鼠骨质疏松的防治有协同效应。

关键词: 葛根异黄酮, 维生素D, 联合作用, 去卵巢, 骨质疏松

Abstract:

BACKGROUND: Total isoflavones of pueraria (TIP) possesses estradiol-like structures and has inhibition effect on bone loss or osteopenia in ovariectomized rats. However, studies have demonstrated that the prevention and treatment effect of TIP on osteoporosis in ovariectomized rats is poor, especially in single utilization.
OBJECTIVE: To investigate the combination effect of TIP and vitamin D on the treatment of osteoporosis in ovariectomized rats.
METHODS: Totally 81 female sprague-drawly (SD) rats of 3-month-old were randomly assigned into 9 groups. Exception those in the sham-surgery group, all rats were prepared for ovariectomized models. Vitamin D or low-, middle- and high-dose TIP or low-, middle- and high-dose TIP combined with vitamin D were intragastric administrated in the vitamin D, TIP or combination groups, respectively. There was no drug medication in the model and sham-surgery groups. The uterus coefficient was calculated at 3 months after medication. The serum alkaline phosphatase (ALP) activity, calcium, phosphonium, bone gla protein, estradiol levels, as well as bone mineral density of femur was determined. 
RESULTS AND CONCLUSION: Compared with the model group, the uterus coefficient and estradiol level was obviously increased in the middle- and high-dose TIP groups and all combination groups (P  < 0.05); the ALP and bone gla protein levels significant decreased (P < 0.05); and the bone mineral density of central and distal femur were notably increased (P  < 0.05); in particular, the result was more manifest in the high-dose combination group. TIP and vitamin D presented with synergism in uterus coefficient, estradiol, ALP, bone gla protein levels and bone mineral density of distal femur, but the effect was not significant in the calcium and phosphonium levels in the TIP and/or vitamin D groups. The findings demonstrated that Tip combined with vitamin D can induce synergism on prevention and cure of osteporosis in ovariectomized rat.

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