中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (1): 100-102.doi: 10.3969/j.issn.1673-8225.2011. 01.022

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

亚甲蓝/光化学法灭活病毒对血浆成分的影响

焦红亮1，2，关方霞3，杨  波1，李建斌2，单  泓2，杜  英4，胡  祥5   

  1. 1郑州大学第一附属医院神经外科，河南省郑州市  450052
    2河南省红十字血液中心，河南省郑州市  450014
    3郑州大学生物医学工程学系，河南省郑州市  450052
    4郑州大学基础医学院微生物与免疫学教研室，河南省郑州市 450052
    5深圳市北科生物科技有限公司，广东省深圳市  518000
  • 收稿日期:2010-10-08 修回日期:2010-11-12 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 杨波，教授，博士生导师，郑州大学第一附属医院神经外科，河南省郑州市 450052 yangbo96@126.com
  • 作者简介:焦红亮☆，男，1978年生，河南省郑州市人，汉族，郑州大学在读博士，主要从事干细胞基础和临床应用方面的研究。 jhl787878@126.com
  • 基金资助:

    郑州大学211工程三期重点学科建设项目；河南省医学科技创新人才工程项目(2005018)。

Effects of methylene blue photochemical virus inactivation on plasma components

Jiao Hong-liang1, 2, Guan Fang-xia3, Yang Bo1, Li Jian-bin2, Shan Hong2, Du Ying4, Hu Xiang5   

  1. 1Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    2Henan Province Red Cross Blood Center, Zhengzhou  450014, Henan Province, China
    3Department of Biomedical Engineering, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    4Department of Microorganism and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    5Beike Bio-Technology, Shenzhen  518000, Guangdong Province, China
  • Received:2010-10-08 Revised:2010-11-12 Online:2011-01-01 Published:2011-01-01
  • Contact: Yang Bo, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Jiao Hong-liang☆, Studying for doctorate, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Henan Province Red Cross Blood Center, Zhengzhou 450014, Henan Province, China jhl787878@126.com
  • Supported by:

    the Key Subject Construction Program of Third-Stage 211 Engineering of Zhengzhou University, the Medical Science and Technology Innovation Talent Engineering Program of Henan Province, No. 2005018

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摘要：

背景：对血液进行病毒灭活是保障安全输血的措施之一，亚甲蓝/光化学法灭活人血浆中病毒的效果已被证实，但其对血浆成分影响的报道很少。
目的：观察亚甲蓝光化学法病毒灭活血浆对血液成分结构和功能的影响。
方法：随机选择40份采血后6 h内400 mL全血制备的新鲜血浆称质量留样，然后与亚甲蓝病毒灭活过滤器无菌连接，亚甲蓝的终浓度在0.9~1.3 μmol/L。将加入亚甲蓝的血浆置入4 ℃病毒灭活箱的搁架上，摆动频率60次/min，利用32 000~ 38 000Lx光照强度的可见光4 ℃照射35 min，将光照后的血浆通过病毒灭活过滤器滤除亚甲蓝和残余白细胞，混匀后留样10 mL，立即置于-80 ℃冰箱冻存。检测照射前后样品的血浆量、亚甲蓝浓度、FⅧ∶C、FⅤ∶C、VWF、Fib含量的变化。
结果与结论：血浆病毒灭活后血浆容量、FⅧ∶C、FⅤ∶C、VWF、Fib的回收率分别为(96.39±1.73)%、(82.55±9.25)%、(81.03±15.27)%、(93.25±6.17)%、(81.61±14.25)%。亚甲蓝光化学法灭活血浆病毒对血浆中大多数成分的影响不明显，可以满足临床安全输血的需求。

关键词: 亚甲蓝, 输血, 凝血因子, 病毒灭活, 血浆

Abstract:

BACKGROUND: Virus inactivation to blood is one of blood transfusion safety measures. The effect of virus inactivation on human plasma by methylene blue/light has been confirmed, but the report concerning virus inactivation to plasma on plasma components is very few.
OBJECTIVE: To observe the effects of methylene blue photochemical virus inactivation to blood plasma on blood components’ structure and function.
METHODS: A total of 40 blood samples were randomly selected. Fresh plasma from 400 mL whole blood within 6 hours was prepared and the quality was weighed for reserve samples, and then the filter with methylene blue virus inactivation was sterilely connected. The final concentration of methylene blue was 0.9-1.3 μmol/L. Plasma added to methylene blue virus inactivation into 4 ℃ was put on a shelf box, with beat frequency of 60 times/minutes. 32 000-38 000 Lx intensity of 4 ℃ visible light was used to irradiate for 35 minutes. The residual methylene blue and white blood cells were filtered out from the plasma after illumination by the virus inactivated filters, mixed and remained sample 10 mL, and immediately placed in -80 ℃ refrigerator. The amount of plasma samples before and after exposure, methylene blue concentration, F Ⅷ: C, F Ⅴ: C, VWF, Fib Content was detected.
RESULTS AND CONCLUSION: After virus inactivated plasma, the recovery rate of plasma volume, F Ⅷ: C, F Ⅴ: C, VWF, Fib were (96.39 ± 1.73)%, (82.55 ± 9.25)%, (81.03 ± 15.27)%, ( 93.25 ± 6.17)%, (81.61 ± 14.25)%. The effect of methylene blue photochemical virus inactivation in plasma on most components of the plasma is not obvious, and meets the clinical demand for safe blood transfusion.

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