中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (32): 5183-5188.doi: 10.3969/j.issn.2095-4344.2017.32.017

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

麦冬多糖对中波紫外线损伤人皮肤成纤维细胞的保护作用

陈高敏，王  璐，杜  沛，李中平，沈红艺   

  1. (上海中医药大学公共健康学院，上海市  201203)
  • 收稿日期:2017-06-12 出版日期:2017-11-18 发布日期:2017-11-15
  • 通讯作者: 沈红艺，博士，研究员，上海中医药大学公共健康学院，上海市 201203
  • 作者简介:陈高敏，女，1990年生，河南省周口市人，汉族，2017年上海中医药大学毕业，主要从事中医营养美容方向的研究。
  • 基金资助:

    卫生局三年行动计划(E2-ED1600103)；上海中医药大学学科建设基金(A1-Z163020508)；上海中医药大学研究生创业专项培养基金(A1-N1501011002)

Polysaccharide from Ophiopogon japonicus protects against ultraviolet B damage to the human dermal fibroblasts

Chen Gao-min, Wang Lu, Du Pei, Li Zhong-ping, Shen Hong-yi   

  1.  (School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China)
  • Received:2017-06-12 Online:2017-11-18 Published:2017-11-15
  • Contact: Shen Hong-yi, M.D., Researcher, School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • About author:Chen Gao-min, School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Supported by:

    the 3-Year Action Program of Health Bureau, No. E2-ED1600103; the Discipline Construction Foundation of Shanghai University of Traditional Chinese Medicine, No. A1-Z163020508; the Graduate Entrepreneurship Training Foundation of Shanghai University of Traditional Chinese Medicine, No. A1-N1501011002

PDF

167

被引次数

10

摘要：

文章快速阅读：


文题释义：
人皮肤成纤维细胞：是真皮层主要的组成细胞，负责分泌前胶原至细胞外，经相关酶的代谢与真皮细胞外基质蛋白结合(例如小分子的蛋白多糖)成三螺旋空间复合体，最终形成结构规则的胶原纤维束，为皮肤提供韧性和抗拉性。对于维持皮肤正常光滑润泽的生理状态具有重要意义。
皮肤光老化：皮肤老化可分为内源性老化和外源性老化。内源性老化指随着时间的推移，由于机体功能下降，皮肤组织呈现的老化。外源性老化由外界众多环境因素引起，其中太阳光中的紫外线是最重要诱导因素。因此，外源性老化又称为光老化。光老化约占人体裸露部位皮肤老化80%。
摘要
背景：随着环境日益恶化，大气臭氧层破坏严重，辐照到地球表面的中波紫外线也日渐增加，防治中波紫外线引起的皮肤光老化意义重大。
目的：观察麦冬多糖对中波紫外线诱导的人皮肤成纤维细胞光老化损伤的保护作用，探讨其作用机制。
方法：实验将人皮肤成纤维细胞分为5组，对照组不光照，无药培养液培养；其余4组均给予200 mJ/cm2的中波紫外线照射建立细胞光老化模型，模型组光照后给予无药培养液培养；各麦冬多糖给药组光照后分别给予10 mg/L、100 mg/L、1 g/L的麦冬多糖培养液培养。培养48 h后CCK-8检测细胞生存率；试剂盒法测定细胞内超氧化物歧化酶、谷胱甘肽过氧化物酶活性及丙二醛含量；利用荧光定量PCR法测定光老化人皮肤成纤维细胞中基质金属蛋白酶1、基质金属蛋白酶3、c-Jun基因表达量。
结果与结论：①200 mJ/cm2的中波紫外线可以降低人皮肤成纤维细胞生存率，超氧化物歧化酶、谷胱甘肽过氧化物酶活性降低，丙二醛含量升高，基质金属蛋白酶1、基质金属蛋白酶3、c-Jun基因表达量增加，与对照组相比差异均有显著性意义(P < 0.05)；②给予10 mg/L、100 mg/L、1 g/L的麦冬多糖均能提高中波紫外线损伤的人皮肤成纤维细胞生存率，升高细胞内超氧化物歧化酶、谷胱甘肽过氧化物酶活性，降低丙二醛含量，降低受损细胞内基质金属蛋白酶1、基质金属蛋白酶3、c-Jun基因表达量；③综上，麦冬多糖能减轻中波紫外线对人皮肤成纤维细胞的损伤，其机制可能是通过缓解中波紫外线诱导的氧化应激，抑制相应信号转导通路，降低光老化细胞内c-Jun表达，进而抑制基质金属蛋白酶1、基质金属蛋白酶3表达，减少皮肤胶原蛋白损伤。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0001-5706-3297(陈高敏)

关键词: 组织构建, 组织工程, 光老化, 中波紫外线, 人皮肤成纤维细胞, 麦冬多糖, 保护

Abstract:

BACKGROUND: With the deterioration of environment, the atmospheric ozone layer is seriously destroyed; thereafter, ultraviolet B (UVB) radiation to the earth surface is increasing. Prevention and control of UVB-induced skin photoaging is of great significance.
OBJECTIVE: To study the protective effect of polysaccharide from Ophiopogon japonicus on the photoaged human dermal fibroblasts
METHODS: Human dermal fibroblasts were divided into five groups: control group received no UVB radiation, and cultured in the free-drug medium; other four groups were irradiated under 200 mJ/cm2 UVB to establish the photoaging model, and then cultured in the drug-free medium (model group), or in the medium of polysaccharide from Ophiopogon japonicus (10, 100, and 1 000 mg/L), respectively. The cell viability was detected by cell counting kit-8 assay after 48-hour culture; the levels of superoxide dismutase, glutathione peroxidase and malondialdehyde were detected by kit method; and the expression levels of matrix metalloproteinases-1 and -3 as well as c-Jun gene were detected by quantitative PCR.
RESULTS AND CONCLUSION: Compared with the control group, 200 mJ/cm2 UVB significantly reduced the viability of human dermal fibroblasts and levels of superoxide dismutase and glutathione peroxidase, as well as increased the malondialdehyde level, and expression levels of matrix metalloproteinase-1 and -3, and c-Jun gene (P < 0.05). In the meanwhile, 10, 100, and 1 000 mg/L polysaccharide from Ophiopogon japonicus significantly reversed all above phenomena (P < 0.05). To conclude, polysaccharide from Ophiopogon japonicas can reduce the damage of UVB to human dermal fibroblasts, maybe by alleviating UVB-induced oxidative stress, inhibiting the relative signaling pathways, and reducing the expression of c-Jun gene, further inhibiting the expression of matrix metalloproteinase-1 and -3, and reducing collagen degradation.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Ultraviolet Rays, Skin, Fibroblasts, Ophiopogon, Tissue Engineering

中图分类号: