中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (29): 4611-4616.doi: 10.3969/j.issn.2095-4344.2017.29.004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞移植修复皮肤创伤

张贝莹，罗冬章，麦海涛，陈祥浪，郭 彦，王丙云，陈胜锋，陈志胜   

  1. 佛山科学技术学院生命科学与工程学院，广东省佛山市  528231
  • 修回日期:2017-05-14 出版日期:2017-10-18 发布日期:2017-11-08
  • 通讯作者: 王丙云，博士，教授，佛山科学技术学院生命科学与工程学院，广东省佛山市 528231
  • 作者简介:张贝莹，女，1993年生，广东省佛山市人，硕士，主要从事兽学专业研究。
  • 基金资助:

    国家自然科学基金(31072101)；佛山市科技创新专项资金项目(佛财工[2013]137号)；2016“攀登计划”广东大学生科技创新培育专项资金项目

Bone marrow mesenchymal stem cell transplantation for skin wound healing

Zhang Bei-ying, Luo Dong-zhang, Mai Hai-tao, Chen Xiang-lang, Guo Yan, Wang Bing-yun, Chen Sheng-feng, Chen Zhi-sheng   

  1. School of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China
  • Revised:2017-05-14 Online:2017-10-18 Published:2017-11-08
  • Contact: Wang Bing-yun, Doctor, Professor, School of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China
  • About author:Zhang Bei-ying, Master, School of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31072101; the Specific Scientific Innovation Fund of Foshan City, No. [2013]137; the 2016 Climbing Plan for Graduates Scientific Innovation Culture in Guangdong Province

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摘要：

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摘要
背景：目前大部分有关间充质干细胞治疗皮肤创伤的实验均使用大鼠、小鼠和兔进行，而应用犬骨髓间充质干细胞治疗皮肤创伤的研究较少。
目的：观察犬骨髓间充质干细胞治疗皮肤创面的效果。
方法：在4只犬肩胛部、臀部两侧分别做3 cm×3 cm的正方形皮肤创伤，右侧作为实验组，在创面制作后的第1，3天，创口皮下注射异体犬骨髓间充质干细胞悬液；左侧作为对照组，在创面制作后的第1，3天，创口皮下注射间充质干细胞培养基，动态观察创面愈合情况。
结果与结论：在第1周时间内，两组创口处都有淡黄色炎性物质存在，炎症反应明显，且对照组炎性物质较实验组多，肉芽组织生长速度慢于实验组；第2周至伤口愈合时间段，创口在形成肉芽组织的基础上开始明显上皮化，主要表现从伤口四周向中央形成新鲜上皮，实验组开始上皮化时间早于对照组，创口面积小于对照组；第3周时，实验组有的创口完全愈合，且愈合后的创面较对照组平整、光滑。实验组细胞移植后8，12 d的创口面积略小于对照组，创口愈合速度稍快于对照组，但差异均无统计学意义。结果表明，犬骨髓间充质干细胞移植具有促进皮肤创面愈合的可能或趋势。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0003-0078-9921(张贝莹)

关键词: 干细胞, 骨髓干细胞, 皮肤创伤, 骨髓间充质干细胞, 犬, 国家自然科学基金

Abstract:

BACKGROUND: At present, most of the experiments on the treatment of skin wound by mesenchymal stem cells have been performed in rats, mice and rabbits, while the research on skin wound treatment by canine bone marrow mesenchymal stem cells is less reported.
OBJECTIVE: To observe the effect of canine bone marrow mesenchymal stem cells on skin wound healing.
METHODS: A 3 cm×3 cm wound was made on the both sides of the scapula and buttocks of the dog, with the right side as experimental group and the left side as control group. After the wound was made, allogeneic canine bone marrow mesenchymal stem cells suspension was injected subcutaneously around the wound in the experimental group on the 1st and 3rd days. The control group was injected subcutaneously around the wound with mesenchymal stem cell culture medium on the 1st and 3rd days after the wound was made. Wound healing was observed dynamically in both groups.
RESULTS AND CONCLUSION: In the 1st week, there were pale yellow inflammatory substances in the wound of two groups indicating obvious inflammations. Compared with the control group, the inflammatory substances were fewer and the growth rate of the granulation tissue was faster in the experimental group. From the 2nd week until the wound healing, epithelialization on the wound became obvious following the formation of the granulation tissue, which was mainly displayed by the formation of fresh epithelial tissues from the surrounding to the wound center. The epithelialization time of the experimental group was earlier than that of the control group, and the wound area of the experimental group was smaller than that of the control group. In the 3rd week, the wound in the experimental group healed completely, and became smoother than that in the control group. The wound area of the experimental group was slightly smaller than that of the control group on the 8th and 12th days after cell transplantation, and the healing speed of the experimental group was slightly faster than that of the control group, but there was no significant difference between the two groups. Our findings indicate that the transplantation of canine bone marrow mesenchymal stem cells has the possibility or trend to promote skin wound healing.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Mesenchymal Stem Cells, Tissue Engineering

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