中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (26): 4172-4177.doi: 10.3969/j.issn.2095-4344.2017.26.013

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

鸡胚尿囊膜载羟基磷灰石血管化模型的建立

任凯歌1，2，郑维银1，2，李  焰2，潘  莲1，张馨月1，田  涛1
  

  1. 1西南医科大学，四川省泸州市  646000；2解放军成都军区总医院附属口腔医院，四川省成都市  610000
  • 收稿日期:2017-04-15 出版日期:2017-09-18 发布日期:2017-09-28
  • 通讯作者: 郑维银，副教授，西南医科大学，四川省泸州市 646000；解放军成都军区总医院附属口腔医院，四川省成都市 610000
  • 作者简介:任凯歌，男，1990年生，四川省绵阳市人，汉族，西南医科大学口腔医学院在读硕士，主要从事口腔颌面外科肿瘤研究。

A revascularization model of chick embryo chorioallantoic membrane carrying hydroxyapatite

Ren Kai-ge1, 2, Zheng Wei-yin1, 2, Li Yan2, Pan Lian1, Zhang Xin-yue1, Tian Tao1
  

  1. 1Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Stomatological Hospital, General Hospital of Chengdu Military Region, Chengdu 610000, Sichuan Province, China
  • Received:2017-04-15 Online:2017-09-18 Published:2017-09-28
  • Contact: Zheng Wei-yin, Associate professor, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Ren Kai-ge, Studying for master’s degree, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Stomatological Hospital, General Hospital of Chengdu Military Region, Chengdu 610000, Sichuan Province, China

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摘要：

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文题释义：
尿囊膜：尿囊膜是鸡胚发育到第四五天时，由被膜尿囊内脏中胚层和绒毛膜中胚层融合而成。鸡胚尿囊膜血管丰富，与绒毛膜血管相通，连接心脏血流，提供丰富营养。由于鸡胚在发育前期缺乏自身免疫系统，故而自1977年Knighton等提出鸡胚尿囊膜模型后，被广泛运用作临床上肿瘤细胞模型的载体。
尿囊膜模型用于血管生成研究的意义：其一，检测评价植入物刺激尿囊膜血管增生的血管生成作用，用于分析研究血管生成促进因子、抑制因子的活性和作用的检测；其二，对植入物，如肿瘤细胞、自身血管生成进行研究，用于分析不同组织的血管生成活性和作用等。
 
背景：利用第8-12天尿囊膜血管的快速生长期，可模拟骨缺损患者骨断端血管生长和植骨代替材料进入人体后的血管植入过程。
目的：将羟基磷灰石负载于鸡胚尿囊膜模型，建立植入体血管化的研究模型。
方法：将12枚罗曼品系鸡蛋于相同适宜条件下孵化至第8天，开窗后随机分为2组，实验组植入羟基磷灰石材料，建立鸡胚尿囊膜载羟基磷灰石模型；对照组放入滤纸材料，密封后移入恒温恒湿箱内继续孵化。待孵化至第12天后，观察两组标本尿囊膜4级以上血管生成长度及单位面积血管生成长度，以及羟基磷灰石及滤纸材料上的血管新生状况。
结果与结论：①待孵化12 d后，实验组标本平均尿囊膜血管新生成长度为24.031 mm，平均单位面积血管新生成长度为0.242 mm/mm2；对照组标本平均尿囊膜血管新生成长度为23.561 mm，平均单位面积血管新生成长度为0.212 mm/mm2，组间血管新生成长度及平均尿囊膜血管新生成长度比较差异无显著性意义；②实验组羟基磷灰石材料切片标本有明显类血管组织；③结果表明，鸡胚尿囊膜载羟基磷灰石模型的建立对理想植骨材料及改变血管生成药物的研究提供了简单准确且高效的体外动物模型，且外载羟基磷灰石对尿囊膜上血管生成无影响。

关键词: 生物材料, 材料相容性, 羟基磷灰石, 尿囊膜, 血管生成, 滤纸

Abstract:

BACKGROUND: Using the rapid growth period of the chorioallantoic membrane from the 8th day to the 12th day, vascular growth at bone defect end and vascular implantation of a bone graft substitute into the human body can be simulated.
OBJECTIVE: To load hydroxyapatite into a chick embryo chorioallantoic membrane model, and to establish an implanted revascularization model.
METHODS: Twelve rosette eggs were incubated under the same suitable conditions for 8 days. After windowing, the eggs were randomly divided into two groups: experimental group was implanted with hydroxyapatite material to establish the model of chick embryo chorioallantoic membrane carrying hydroxyapatite; control group was implanted with filter paper material, sealed and placed into a constant temperature and humidity box to continue to hatch. At the 12th day of incubation, length of new vessels and length of new vessels per unit area in the two groups were observed, and the angiogenesis on the hydroxyapatite and filter paper was observed.
RESULTS AND CONCLUSION: The mean length of new vessels and length of new vessels per unit area were 24.031 mm and 0.242 mm/mm2 in the experimental group, and 23.561 mm and 0.212 mm/mm2 in the control group, respectively. There were no significant differences between two groups. Additionally, vascular tissues were obviously observed on the hydroxyapatite samples in the experimental group. These experimental results show that the establishment of the model of chick embryo chorioallantoic membrane carrying hydroxyapatite provides a simple and accurate in vitro animal model for studies on ideal bone graft materials and angiogenesis. The hydroxyapatite has no effect on the angiogenesis on the chorioallantoic membrane.

Key words: Hydroxyapatites, Neovascularization, Physiologic, Tissue Engineering

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