中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (25): 3949-3955.doi: 10.3969/j.issn.2095-4344.2017.25.003

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

脐带间充质干细胞对NB4白血病细胞基因组表达谱的影响

范会芳,陈 芳,马凤霞,池 颖,卢士红,韩忠朝   

  1. 中国医学科学院、北京协和医学院血液学研究所、血液病医院,实验血液学国家重点实验室,天津市  300020
  • 修回日期:2017-07-14 出版日期:2017-09-08 发布日期:2017-10-09
  • 通讯作者: 韩忠朝,教授,博士生导师,中国医学科学院、北京协和医学院血液学研究所、血液病医院,实验血液学国家重点实验室,天津市 300020
  • 作者简介:范会芳,女,1990年生,河北省邢台市人,汉族,中国医学科学院、北京协和医学院血学研究所、血液病医院,实验血液学国家重点实验室在读硕士,主要从事间充质干细胞与血液病方面的研究。
  • 基金资助:

    国家自然科学基金(81500098和81330015);中国医学科学院医学与健康科技创新工程(2016-I2M-1-017)

Genome-wide transcriptional profiling of NB4 leukemic cells affected by umbilical cord-derived mesenchymal stem cells

Fan Hui-fang, Chen Fang, Ma Feng-xia, Chi Ying, Lu Shi-hong, Han Zhong-chao   

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
  • Revised:2017-07-14 Online:2017-09-08 Published:2017-10-09
  • Contact: Han Zhong-chao, Professor, Doctoral supervisor, State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, Chin
  • About author:Fan Hui-fang, Studying for master′s degree, State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81500098, 81330015; the CAMS Initiative for Innovative Medicine, No. 2016-I2M-1-017

摘要:

文章快速阅读:

 

文题释义:
基因芯片(又称 DNA 芯片、生物芯片):
该技术系指将大量(通常每平方厘米点阵密度高于400)探针分子固定于支持物上然后与标记的样品分子进行杂交,通过检测每个探针分子的杂交信号强度进而获取样品分子的数量和序列信息。通俗地说,就是通过微加工技术,将数以万计、乃至百万计的特定序列的DNA片段(基因探针),有规律地排列固定于2 cm2的硅片、玻片等支持物上,构成的一个二维DNA探针阵列,与计算机的电子芯片十分相似,所以被称为基因芯片。基因芯片主要用于基因检测工作。
移植物抗宿主病:是异基因造血干细胞移植后特有的并发症,是移植治疗相关死亡主要原因之一,由供者T细胞攻击受者同种异型抗原所致。产生移植物抗宿主病的危险因素包括:供受者间HLA相合程度、有无血缘关系、性别差异、年龄、基础疾病及其所处状态、预处理方式、移植物抗宿主病预防方案、移植物特性、感染、组织损伤等。

 

摘要
背景:
间充质干细胞是体内微环境的重要组成部分且影响肿瘤细胞的多种生物学行为,间充质干细胞的潜在临床价值是近年来的研究热点。
目的:通过基因芯片方法分析脐带间充质干细胞对急性早幼粒细胞白血病细胞系NB4 细胞基因组表达谱的影响。
方法:体外建立脐带间充质干细胞与急性早幼粒细胞白血病细胞系NB4细胞共培养体系,检测脐带间充质干细胞对NB4细胞增殖、凋亡和分化的影响,分别提取了NB4细胞单独培养组和NB4细胞+脐带间充质干细胞共培养组NB4细胞的mRNA并将其反转录为cDNA,两组分别标记荧光,做成探针并与基因芯片杂交,检测其荧光强度,分析两组基因表达谱的差异。
结果与结论:①脐带间充质干细胞促进NB4细胞的增殖和分化并抑制其凋亡;②脐带间充质干细胞作用后的NB4细胞的基因表达谱中有530个基因上调和53个基因下调,与基因相关的功能和信号通路也在一定程度上受到了调节;③结果表明,脐带间充质干细胞通过改变肿瘤细胞内大量基因表达、基因功能及多种信号通路影响NB4细胞的生物学行为。

 

关键词: 干细胞, 脐带脐血干细胞, 白血病, NB4细胞, 脐带间充质干细胞, cDNA芯片, 国家自然科学基金

Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) are an important component of the in vivo microenvironment and act on multiple biological behaviors of tumor cells. The potential clinical value of MSCs has become an issue of concern in recent years.
OBJECTIVE: To investigate the gene expression profiles of acute promyelocytic leukemia (APL) cell line NB4 treated with umbilical cord-derived MSCs (UC-MSCs) using cDNA microarray.
METHODS: In vitro co-culture system was constructed, and then cellular proliferation, apoptosis and differentiation status of NB4 cells treated with UC-MSCs were evaluated. Two cDNA probes were prepared through reverse transcription from mRNA of NB4 cells treated with or without UC-MSCs. The probes were labeled with fluorescence dyes individually, hybridized with cDNA microarray, and their fluorescent intensities were scanned. The genes were screened through the analysis of the difference in two gene expression profiles.
RESULTS AND CONCLUSION: UC-MSCs promoted the proliferation and differentiation, while reduced the apoptosis of NB4 cells. The analysis of gene expression profiles indicated that after co-culture with UC-MSCs, 530 genes were up-regulated and 53 genes were down-regulated. Accordingly, specific gene function and pathway signaling related were also regulated to some extent. Overall, UC-MSCs influence can major biological behaviors of NB4 cells by changing expression of a large amount of genes, gene-related function and multiple intracellular signaling pathways. 

 

Key words: Umbilical Cord, Mesenchymal Stem Cells, Leukemia, Promyelocytic, Acute, Microchip Analytical Procedures, Tissue Engineering

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