中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (24): 3888-3893.doi: 10.3969/j.issn.2095-4344.2017.24.020

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

基于力生长因子的力生物学骨重建模型

马宗民,李淑娴   

  1. 大连大学机械工程学院,辽宁省大连市 116622
  • 修回日期:2017-04-25 出版日期:2017-08-28 发布日期:2017-08-30
  • 通讯作者: 马宗民,博士,讲师,大连大学机械工程学院,辽宁省大连市 116622; 并列通讯作者:李淑娴,讲师,大连大学机械工程学院,辽宁省大连市 116622
  • 作者简介:马宗民,男,1974年生,山东省兖州市人,汉族,2005年吉林大学毕业,博士,讲师,主要从事生物力学、医疗器械研究。
  • 基金资助:

    国家自然科学基金项目(11572066,11602047)

Mechanobiological model of bone remodeling based on mechano-growth factors

Ma Zong-min, Li Shu-xian   

  1. Mechanical Engineering College of Dalian University, Dalian 116622, Liaoning Province, China
  • Revised:2017-04-25 Online:2017-08-28 Published:2017-08-30
  • Contact: Ma Zong-min, Mechanical Engineering College of Dalian University, Dalian 116622, Liaoning Province, China; Li Shu-xian, Lecturer, Mechanical Engineering College of Dalian University, Dalian 116622, Liaoning Province, China
  • About author:Ma Zong-min, M.D., Lecturer, Mechanical Engineering College of Dalian University, Dalian 116622, Liaoning Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 11572066 and 11602047

摘要:

文章快速阅读:

文题释义:
力生长因子(mechano-growth factor,MGF):
是力敏感细胞响应机械刺激而产生的一种力效应分子。许多研究表明,力生长因子在多种组织/细胞中表达,具有力敏感性,并且能够激活卫星细胞,促进成肌细胞增殖,在治疗肌肉缺损、预防心肌损伤和修复受损神经等方面起着重要作用;对骨组织有类似力学刺激的调控作用,对骨骼的重建和修复有重要作用。
力学生物学:20世纪90年代中期年以来,随着科学技术的进步,生物力学研究也逐渐深入到细胞分子层次,形成了新的学科分支领域——力学生物学(mechanobiology)。力学生物学是探讨力学环境(刺激)对生物体健康、疾病或损伤的影响,研究生物体的力学信号感受和响应机制,阐明机体的力学过程与生物学过程如生长、重建、适应性变化和修复等之间的相互关系,从而发展有疗效的或有诊断意义的新技术,促进生物医学基础与临床研究的发展,促进人类健康。

 

摘要
背景:
力学信号传导是应力与生长关系中研究热点。力生长因子是一种应力敏感因子,力学刺激作用下在成骨细胞中表达,与应力作用对骨代谢的调控有类似的效应。
目的:探讨骨重建过程中力学信号的传导,在细胞分子水平揭示应力与生长的关系。
方法:建立力生长因子与力学激励关系控制方程、力生长因子对成骨细胞和破骨细胞调控作用控制方程、力生长因子对RANK-RANKL-OPG信号轴调控作用控制方程,进而建立以力生长因子作为介导的骨重建力生物学数学模型,并应用该模型进行了力学刺激下骨重建过程的模拟。
结果与结论:①废用时,成骨细胞/破骨细胞比值降低,骨平衡为负,骨量减少,骨体积分数降低;②过载时,成骨细胞/破骨细胞比值升高,骨平衡为正,骨量增加,骨体积分数增加。模拟结果符合Frost力学调控系统理论;③结论:以力生长因子作为力生物学信号的传导的介导,提出力生物学骨重建数学模型,实现了骨重建过程中力生物学信号的转导。

 

关键词: 组织构建, 骨组织工程, 力生长因子, 骨重建, 数学模型, 力学信号传导, 国家自然科学基金项目

Abstract:

BACKGROUND: Mechanotransduction is an issue of concern in the study on the relationship between stress and growth. Mechano-growth factor (MGF) holds stress sensitivity, and exerts similar effect with stress in bone metabolism regulation.

OBJECTIVE: To explore the mechanotransduction during bone remodeling, and investigate the relationship between stress and growth at molecular level.
METHODS: The governing equations about the relationship between MGF and mechanical stimulation, regulation of MGF on osteoblasts and osteoclasts, regulation of MGF on RANK-RANKL-OPG signaling axis were established, and then the MGF-mediated bone reconstruction model was established to simulate the bone remodeling process under mechanical stimulation.
RESULTS AND CONCLUSION: Under the condition of disuse, there was a decrease in osteoblasts/osteoclasts ratio, bone mass and bone volume fraction, and bone resorption was more than bone formation. Under the condition of overload, there was an increase in osteoblasts/osteoclasts ratio, bone mass and bone volume fraction, and bone formation was more than bone resorption. The simulation results were in accordance with Frost mechanostat theory. These findings show that the mechanobiological model of bone remodeling based on MGF can simulate the bone remodeling process under mechanical stimuli, and achieve mechanotransduction.

Key words: Biomechanics, Signal Transduction, Osteoblasts, Osteoclasts, Tissue Engineering

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