中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (24): 3833-3838.doi: 10.3969/j.issn.2095-4344.2017.24.011

• 神经组织构建 nerve tissue construction • 上一篇    下一篇

改良兔防御素1对周围神经损伤修复后神经再生的作用

徐春归,张积森,许新忠,荆珏华   

  1. 安徽医科大学第二附属医院,安徽省合肥市 230041
  • 修回日期:2017-07-01 出版日期:2017-08-28 发布日期:2017-08-30
  • 通讯作者: 荆珏华,博士,主任医师,副教授,硕士生导师,安徽医科大学第二附属医院骨科,安徽省合肥市 230041
  • 作者简介:徐春归,男,1984年生,安徽省人,汉族,2016年北京大学毕业,博士,主要从事骨科和周围神经损伤再生方面的研究。
  • 基金资助:

    安徽省自然科学基金项目(1608085QH206);安徽医科大学校科研基金(2015xkj017)

Effect of modified rabbit defensin 1 on peripheral nerve regeneration

Xu Chun-gui, Zhang Ji-sen, Xu Xin-zhong, Jing Jue-hua   

  1. the Second Hospital of Anhui Medical University, Hefei 230041, Anhui Province, China
  • Revised:2017-07-01 Online:2017-08-28 Published:2017-08-30
  • Contact: Jing Jue-hua, M.D., Chief physician, Associate professor, Master’s supervisor, the Second Hospital of Anhui Medical University, Hefei 230041, Anhui Province, China
  • About author:Xu Chun-gui, M.D., the Second Hospital of Anhui Medical University, Hefei 230041, Anhui Province, China
  • Supported by:

    the Natural Science Foundation of Anhui Province, No. 1608085QH206; the Research Foundation of Anhui Medical University, No. 2015xkj017

摘要:

文章快速阅读:

文题释义:
防御素:
是存在于脊椎动物和无脊椎动物体内的一类富含半胱氨酸的阳离子蛋白,在植物中也有类似蛋白。它们的功能主要是宿主防御。它们可以抵御细菌、真菌以及多种有或无包膜的病毒。通常由18-45个氨基酸构成,包含6-8个保守的半胱氨酸序列。免疫系统的细胞分泌这类蛋白来帮助消灭被吞噬的细菌,例如中性粒细胞和表皮细胞吞噬的细菌。
神经再生:是指神经组织、细胞或细胞产物的再生或修复。其机制包括新的神经元、神经胶质、轴突、髓鞘或突触的再生。中枢神经系统和周围神经系统的神经再生,其机制、再生速度以及再生程度都有所不同。周围神经轴突损伤后,远端神经经历华勒变性,近端神经可能凋亡坏死或染色体溶解反应,进而开始修复。

 

摘要
背景:
周围神经损伤是临床常见的一类疾病,严重影响患者的生活质量。如何促进周围神经损伤后神经的再生修复是临床重要的课题。
目的:探讨改良兔防御素1对周围神经损伤修复后神经再生的作用及功能恢复的影响。
方法:18只SD大鼠随机分为3组:神经生长因子神经营养因子组、改良兔防御素1组以及生理盐水对照组。将所有大鼠坐骨神经离断后使用可降解甲壳质生物套管套接修复神经断端,术后连续7 d以神经营养因子、改良兔防御素1以及生理盐水注射到臀肌中。
结果与结论:术后6周,神经营养因子组和改良兔防御素1组大鼠的坐骨神经指数较生理盐水组高,但3组大鼠的运动神经传导速度:神经营养因子组> 兔防御素1组> 生理盐水组,且改良兔防御素1组大鼠的再生神经纤维直径、轴突直径和髓鞘厚度较神经营养因子组大鼠小,但相对于生理盐水组大。提示改良兔防御素1对于周围神经损伤后神经再生具有促进作用。改良兔防御素1促进神经再生的作用机制可能与巨噬细胞清理髓鞘残留、改善神经再生环境有关。

 

关键词: 组织构建, 组织工程, 防御素, 周围神经再生, 坐骨神经指数, 运动神经传导速度, 再生神经纤维直径, 轴突直径, 髓鞘厚度, G-ratio, 安徽省自然科学基金

Abstract:

BACKGROUND: Peripheral nerve injury is a common disease in clinic, which severely affects the patients’ quality of life. How to promote peripheral nerve regeneration is an issue of concern.
OBJECTIVE: To study the effect of modified rabbit defensin 1 on peripheral nerve regeneration and functional recovery.
METHODS: Eighteen Sprague-Dawley rats were randomly divided into three groups. The rat sciatic nerves were transected and bridged by biodegenerated chitin conduits, followed by the injection of neurotrophic factor (group 1), modified rabbit defensin 1 (group 2) and normal saline (control group) into the gluteus, respectively, for consecutive 7 days.
RESULTS AND CONCLUSION: The sciatic nerve function index in the groups 1 and 2 was higher than that in the control group at 4 weeks postoperatively. The order of motor nerve conductive velocity was as follows: group 1 > group 2 > control group. The diameter regenerated fibers and axons, and the myelin thickness in the group 2 were less than those in the group 1, but were more than those in the control group. These results indicate that the modified rabbit defensin 1 can promote peripheral nerve regeneration, which may be related with the clearance of residual myelin by macrophages and the improvement in nerve regeneration environment.

 

Key words: Defensins, Macrophages, Tissue Engineering

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