中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (20): 3183-3189.doi: 10.3969/j.issn.2095-4344.2017.20.012

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

腺病毒介导神经生长因子和髓磷脂相关糖蛋白双基因在受损坐骨神经中的表达

陈  渝1,邓忠良2,陈诗谋1,翁  政1,黄  方3   

  1. 1重庆市第九人民医院骨科,重庆市  400700;2重庆医科大学附属第二医院骨科,重庆市  400010;3重庆市人民医院呼吸内科,重庆市  400014
  • 修回日期:2017-05-24 出版日期:2017-07-18 发布日期:2017-07-28
  • 通讯作者: 黄方,硕士,副主任医师,重庆市人民医院呼吸内科,重庆市400014
  • 作者简介:陈渝,男,1977年生,四川省资阳县人,汉族, 2011年重庆医科大学毕业,博士,副主任医师,主要从事骨关节疾病及周围神经损伤的研究。
  • 基金资助:

    国家自然科学基金项目(81272005);重庆市北碚区科委重点课题(2008-10)

Expression of adenovirus-mediated nerve growth factor and myelin-associated glycoprotein double-gene in sciatic nerve injury

Chen Yu1, Deng Zhong-liang2, Chen Shi-mou1, Weng Zheng1, Huang Fang3   

  1. 1Department of Orthopedics, the Ninth People’s Hospital of Chongqing, Chongqing 400700, China; 2Department of Orthopedics, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; 3Department of Respiratory, Chongqing General Hospital, Chongqing 400014, China
  • Revised:2017-05-24 Online:2017-07-18 Published:2017-07-28
  • Contact: Huang Fang, Master, Associate chief physician, Department of Respiratory, Chongqing General Hospital, Chongqing 400014, China
  • About author:Chen Yu, M.D., Associate chief physician, Department of Orthopedics, the Ninth People’s Hospital of Chongqing, Chongqing 400700, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81272005; the Key Project of Chongqing Beibei District Science Committee, No. 2008-10

摘要:

文章快速阅读:

文题释义:
神经生长因子:
是一类能促进神经生长的多肽,具有调控氨基酸和小分子的摄入,增强功能蛋白和结构蛋白质合成,促进神经修复与再生的功能,对神经系统的发育及促进再生等方面均有重要的生物学作用,是神经系统中最重要的生物活性物质之一。但目前研究表明,其促进神经纤维再生的同时,却无法维持神经纤维的正常结构。
髓磷脂相关糖蛋白:是一种定位于髓鞘轴突旁的Schwanm细胞和少突胶质细胞上的跨膜糖蛋白,属于免疫球蛋白超家族中的唾液酸亚群,包含着5个免疫球蛋白样区域,在胶质和轴突之间发挥作用。它是一个双功能蛋白,对早期发育的神经轴突具有促进生长作用,而对成熟轴突的过度生长具有抑制作用。

 

摘要
背景:
神经生长因子和髓磷脂相关糖蛋白能够促进损伤神经的结构与功能恢复,但目前未见通过转基因技术增加受损周围神经中神经生长因子与髓磷脂相关糖蛋白基因表达的报道。
目的:探讨神经生长因子和髓磷脂相关糖蛋白双基因经腺病毒介导在大鼠损伤坐骨神经的共表达效果。 
方法:将40只SD大鼠随机分为正常对照组、坐骨神经损伤组、空病毒组、神经生长因子组和神经生长因子/髓磷脂相关糖蛋白组。后4组切断并缝合右侧坐骨神经建立坐骨神经损伤模型后,分别肌肉注射生理盐水、生理盐水、空腺病毒(1×108 PFU/次)、携带神经生长因子的腺病毒(1×108 PFU/次)和携带神经生长因子和髓磷脂相关糖蛋白的腺病毒(1×108 PFU/次),每2 d注射1次,连续注射3次。

结果与结论:神经生长因子组和神经生长因子/髓磷脂相关糖蛋白组大鼠坐骨神经中神经生长因子表达水平显著高于空病毒组和正常对照组(P < 0.05);神经生长因子/髓磷脂相关糖蛋白组大鼠坐骨神经中髓磷脂相关糖蛋白表达水平显著高于空病毒组、神经生长因子组和正常对照组(P < 0.05)。提示腺病毒介导的神经生长因子/髓磷脂相关糖蛋白双基因可有效表达于大鼠损伤坐骨神经。

 

 

ORCID: 0000-0002-7156-778X(黄方)

关键词: 组织构建, 组织工程, 坐骨神经损伤, 神经生长因子, 髓磷脂相关糖蛋白, 腺病毒, 神经修复, 周围神经, 基因技术, 国家自然科学基金

Abstract:

BACKGROUND: Nerve growth factor (NGF) and myelin-associated glycoprotein (MAG) can promote the repair of peripheral nerve. However, increasing the expression of NGF and MAG in the injured peripheral nerve by transgene technology has not been reported.

OBJECTIVE: To investigate the expression of NGF and MAG after adenovirus (Ad) mediated transfection in the injured sciatic nerve in rats.
METHODS: Forty Sprague-Dawley rats were randomly divided into normal, model, Ad, NGF and NGF-MAG groups. Sciatic nerve injury models were established in the latter four groups by cutting and suturing the right sciatic nerve. Afterwards, the rats in each group were subject to the intramuscular injection of normal saline, normal saline, Ad (1×108 PFU), Ad-NGF (1×108 PFU), and Ad-NGF-MAG (1×108 PFU), respectively, once every 2 days, for consecutive three times.

RESULTS AND CONCLUSION: The expression level of NGF in the rat sciatic nerve in the Ad and normal groups was significantly higher than that in the NGF and NGF-MAG groups (P < 0.05). Compared with the Ad, NGF and normal groups, the expression level of MAG in the NGF-MAG group was significantly increased (P < 0.05). To conclude, the Ad-mediated NGF and MAG after double gene transfection were expressed effectively in the injured sciatic nerve in rats.

 

 

Key words: Nerve Growth Factor, Myelin-Associated Glycoprotein, Transfection, Tissue Engineering

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