中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (2): 249-253.doi: 10.3969/j.issn.2095-4344.2017.02.016

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

釉基质蛋白对双重酸蚀后纯钛表面磷灰石生成的影响

朱习华1，吴倩雯2，黄  慧3
  

  1. 1武汉科技大学附属天佑医院口腔科，湖北省武汉市  430064；2上海市东方医院口腔科，上海市  200011；3上海交通大学医学院附属第九人民医院口腔修复科，上海市  200011
  • 收稿日期:2016-10-28 出版日期:2017-01-18 发布日期:2017-02-27
  • 通讯作者: 黄慧，博士，副主任医师，上海交通大学医学院附属第九人民医院口腔修复科，上海市 200011
  • 作者简介:朱习华，女，1966年生，湖北省人，汉族，1990年武汉大学毕业，副主任医师，主要从事口腔修复研究。 并列第一作者：吴倩雯，硕士，主治医师，上海市东方医院口腔科，上海市200011
  • 基金资助:

    国家自然科学基金项目(30670555)

Effect of enamel matrix proteins on the growth of apatite coating on dual thermo-etching modified titanium

Zhu Xi-hua1, Wu Qian-wen2, Huang Hui3 
  

  1. 1Department of Stomatology, Tianyou Hospital Affiliated to Wuhan University of Science & Technology, Wuhan 430064, Hubei Province, China; 2Department of Stomatology, Dongfang Hospital of Shanghai, Shanghai 200011, China; 3Department of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Received:2016-10-28 Online:2017-01-18 Published:2017-02-27
  • Contact: Huang Hui, M.D., Associate chief physician, Department of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • About author:Zhu Xi-hua, Associate chief physician, Department of Stomatology, Tianyou Hospital Affiliated to Wuhan University of Science & Technology, Wuhan 430064, Hubei Province, China Wu Qian-wen, Master, Attending physician, Department of Stomatology, Dongfang Hospital of Shanghai, Shanghai 200011, China Zhu Xi-hua and Wu Qian-wen contributed equally to this work.
  • Supported by:

    the National Natural Science Foundation of China, No. 30670555

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摘要：

文章快速阅读：

 

文题释义：
釉基质蛋白：存在于发育期的釉基质中，其作用与釉基质的形成和矿化有关。釉基质中的蛋白主要包括两种：即釉原蛋白和非釉原蛋白，其中釉原蛋白占90%，由一组蛋白及多肽组成。发育中的釉质基质的主要成分是釉原蛋白，除了在釉质的成熟和矿化中发挥作用外，还能诱导无细胞性牙骨质的形成。
磷灰石：釉基质蛋白分子与羟基磷灰石具有亲和性，釉基质蛋白对羟基磷灰石晶体有选择性吸附作用，实验通过模拟生物矿化的过程，将纯钛试件浸入到加有釉基质蛋白的钙磷过饱和液中，以考察釉基质蛋白是否影响纯钛表面矿化涂层的生成。

背景：为提高钛种植体植入后的生物活性，常采用各种表面改性技术。
目的：研究釉基质蛋白对双重热酸蚀纯钛表面仿生矿化沉积磷灰石层的影响。
方法：提取猪未萌恒牙胚表面的釉基质蛋白并进行验证，纯钛片经抛光清洗后进行双重热酸蚀处理，然后放入含不同浓度釉基质蛋白的饱和矿化液中矿化7 d，对照组为不含釉基质蛋白的钙磷过饱和液。扫描电镜观察试件表面形貌，X射线能谱及X射线衍射分析涂层的元素成分及晶相结构。
结果与结论：经双重酸蚀处理后的钛片表面形成规则凹坑状的粗糙表面，经过7 d的矿化实验后，不添加釉基质蛋白的对照组试件上未见明显矿化涂层的形成。加入釉基质蛋白的实验组试件表面可形成矿化涂层，且随釉基质蛋白浓度的增加，矿化涂层的量及形态发生改变。能谱分析结果显示：矿化涂层中的主要元素为钙、磷、碳和氧，钙磷摩尔比在1.32-1.41之间。X射线衍射结果显示纯钛试件表面的沉积物为碳羟基磷灰石。提示釉基质蛋白可以促进双重酸蚀处理后的纯钛表面更快地生成磷灰石涂层。釉基质蛋白对纯钛表面磷灰石涂层的影响呈浓度相关性。

关键词: 生物材料, 口腔生物材料, 釉基质蛋白, 纯钛, 磷灰石, 牙种植体, 模拟体液, 双重酸蚀, 扫描电子显微镜, X射线衍射, 国家自然科学基金

Abstract:

BACKGROUND: Various surface modification techniques have been used to improve the bioactivity of titanium
implant in vivo.
OBJECTIVE: To investigate the effects of enamel matrix proteins (EMPs) on the growth of apatite coatings on dual thermo-etching treated pure titanium.
METHODS: EMPs were extracted from porcine tooth germs and then were identified. Dual thermo-etching was applied to treat titanium samples following polished, and then immersed in a blank simulated body fluid supersaturated calcification solution (control group) or supersaturated calcification solution containing different concentrations of EMPs for 7 days. The morphology of samples was observed using scanning electron microscope, and element components and crystal structures of the apatite coatings were analyzed by energy dispersive spectrometer and X-ray diffraction.
RESULTS AND METHODS: After double-etching, a pit-like rough surface was observed on the titanium plate. After 7-day mineralization, in the control group, no overt calcium-phosphate deposits were found on the titanium surface; however, in the experimental groups, there were calcium-phosphate deposits, whose quantity and morphology changed with increasing concentrations. Energy dispersive spectrometer showed that the main element components of the mineralized coating included calcium, phosphorus, oxygen and carbon, and the calcium-phosphate ratio ranged from 1.32 to 1.41. The apatite coatings were proved to be carbonate hydroxyapatite by X-ray diffraction. To conclude, EMPs promote apatited deposition on pure titanium surfaces in a concentration-dependent manner.

Key words: Titanium, Dental Enamel, Tissue Engineering

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