中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (21): 3097-3103.doi: 10.3969/j.issn.2095-4344.2016.21.008

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

万古霉素/羟基磷灰石/钛金属纳米管的生物相容性

张杭州1,田 昂2,梁庆威1,白希壮3,薛向欣2   

  1. 1中国医科大学附属第一医院运动医学科/关节外科,辽宁省沈阳市  1100012东北大学材料与冶金学院,辽宁省沈阳市 110016;3中国医科大学附属人民医院运动医学科/关节外科,辽宁省沈阳市  110006
  • 收稿日期:2016-03-13 出版日期:2016-05-20 发布日期:2016-05-20
  • 作者简介:张杭州,男,1984年生,山东省淄博市人,汉族,博士,主治医师,主要从事运动关节损伤及骨科新型抗菌内植物研究。
  • 基金资助:

    国家自然科学基金(81071449; 81501857);辽宁省省直医院改革重点临床科室诊疗能力建设项目(LNCCC-A03-2014)

 Biocompatibility of vancomycin/hydroxyapatite/titanium nanotubes

Zhang Hang-zhou1, Tian Ang2, Liang Qing-wei1, Bai Xi-zhuang3, Xue Xiang-xin2   

  1. 1Department of Sports Medicine and Joint Surgery, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China; 2School of Materials and Metallurgy, Northeastern University, Shenyang 110016, Liaoning Province, China; 3Department of Sports Medicine and Joint Surgery, People’s Hospital of China Medical University, Shenyang 110006, Liaoning Province, China
  • Received:2016-03-13 Online:2016-05-20 Published:2016-05-20
  • About author:Zhang Hang-zhou, M.D., Attending physician, Department of Sports Medicine and Joint Surgery, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81071449, 81501857; Provincial-Level Hospital Reform Project for Construction of Key Clinical Department in Liaoning Province, No. LNCCC-A03-2014

摘要:

 文章快速阅读:

文题释义:
纯钛金属及其合金:
钛及其合金因具有相对较低的弹性模量,良好的抗疲劳强度、可塑性强和加工性,耐腐蚀性等优良性质,作为骨科植入材料被广泛应用在组织工程领域。纯钛金属及其合金在空气暴露下自发形成了二氧化钛氧化膜, 氧化钛氧化膜虽然增加了材料本身的耐腐蚀性,但氧化膜本身具有生物惰性。为了增加目前生物材料的生物活性,常采用生物涂层技术及材料表面处理(金属表面改性)。
万古霉素/羟基磷灰石/钛金属纳米管:在二氧化钛纳米管共沉积羟基磷灰石及庆大霉素后,能够显著延缓药物的释放时间。二氧化纳米管表面沉积羟基磷灰石后载药,比纳米管单纯载药的释放模式更为舒缓。由于纳米羟基磷灰石表面常能形成较高的钙离子浓度环境,因而在体液中可形成微碱性环境,以促进成骨细胞的黏附、生长和分化,达到更好的成骨作用。作为抗骨感染的药物缓释系统,万古霉素/羟基磷灰石/钛金属纳米管系统作为一种支架材料,允许成骨细胞长入,促进骨修复。

背景:为了克服单一材料的缺点,羟基磷灰石/金属基复合材料同时负载抗生素的研究已引起人们的关注。
目的:检测万古霉素/羟基磷灰石/钛金属纳米管的生物相容性。
方法:将商业钛金属、二氧化钛纳米管及万古霉素/羟基磷灰石/钛金属纳米管分别与小鼠成骨细胞系MC-3T3-E1共培养,培养1,3,5 d时,倒置显微镜、扫描电镜下观察细胞生长情况;培养1,3,5 d时,AO-EB法检测细胞增殖;培养7,14 d时,检测细胞内总蛋白、钙与碱性磷酸酶水平。
结果与结论:与商业钛金属组、二氧化钛纳米管组比较,万古霉素/羟基磷灰石/钛金属纳米管组小鼠成骨细胞MC-3T3-E1黏附良好,细胞活性及细胞形态良好,细胞生出大量伪足黏附于复合物表面;万古霉素/羟基磷灰石/钛金属纳米管组细胞数量多于商业钛金属组、二氧化钛纳米管组,细胞内钙、碱性磷酸酶水平高于商业钛金属组、二氧化钛纳米管组。结果表明,万古霉素/羟基磷灰石/钛金属纳米管具有良好的生物相容性,无生物毒性。

 

 ORCID: 0000-0001-8233-5362(张杭州)

关键词: 生物材料, 材料相容性, 万古霉素, 羟基磷灰石, 钛纳米管, 成骨细胞, 国家自然科学基金

Abstract:

BACKGROUND: In order to overcome the shortcomings of single materials, antibiotics-loaded hydroxyapatite/titanium composites have attracted people’s attentions.

OBJECTIVE: To evaluate the biocompatibility of vancomycin/hydroxyapatite/titanium nanotubes.
METHODS: Mouse osteoblasts, MC-3T3-E1, were co-cultured with titanium (Cp-T), TiO2 nanotubes, and vancomycin/hydroxyapatite/titanium nanotubes, respectively. Cell morphology and growth were observed after 1, 3 and 5 days of co-culture under inverted microscope and scanning electron microscope. The cell proliferation was detected by AO-EB method. The total protein, calcium and alkaline phosphatase levels were detected at 7 and 14 days of co-culture.

RESULTS AND CONCLUSION: The MC-3T3-E1 cells with good viability and morphology adhered well on the surface of vancomycin/hydroxyapatite/titanium nanotubes compared with those on the surface of pure titanium and TiO2 nanotubes under the scanning electron miscroscope. Moreover, there were a large amount of pseudopodia on the surface of composite nanotubes. Compared with the other two groups, the cell number on the surface and the levels of intracellular calcium and alkaline phosphatase were all higher in the vancomycin/hydroxyapatite/titanium nanotubes group. These findings suggest that the vancomycin/hydroxyapatite/titanium nanotubes have good biocompatibility and no cytotoxicity.

 

Key words: Vancomycin, Durapatite, Nanotubes, Tissue Engineering

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