中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (18): 2655-2660.doi: 10.3969/j.issn.2095-4344.2016.18.011

• 周围神经损伤动物模型 Animal models of peripheral nerve injury • 上一篇    下一篇

皮质酮损害大鼠新颖物体识别记忆的再巩固

周梅芳1，王 波1，田绍文2，谭德容1，旷 昕1   

  1. 1南华大学附属第一医院麻醉科，湖南省衡阳市 421001；2南华大学医学院生理学教研室，湖南省衡阳市 421001
  • 收稿日期:2016-03-08 出版日期:2016-04-29 发布日期:2016-04-29
  • 通讯作者: 旷昕，博士，主任医师，副教授，硕士生导师，南华大学附属第一医院麻醉科，湖南省衡阳市 421001
  • 作者简介:周梅芳，女，1983年生，南华大学附属第一医院在读硕士，主要从事神经与认知功能研究。
  • 基金资助:

    国家自然科学基金(30770689，81171281)；湖南省自然科学基金重点项目(10JJ2009)；湖南省衡阳市科学技术局一般项目(2012KJ41)

Corticosterone impairs reconsolidation of novel object recognition memory in rats

Zhou Mei-fang1, Wang Bo1, Tian Shao-wen2, Tan De-rong1, Kuang Xin1   

  1. 1Department of Anesthesiology, First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China; 2Department of Physiology, Medical School, University of South China, Hengyang 421001, Hunan Province, China
  • Received:2016-03-08 Online:2016-04-29 Published:2016-04-29
  • Contact: Kuang Xin, M.D., Chief physician, Associate professor, Master’s supervisor, Department of Anesthesiology, First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China
  • About author:Zhou Mei-fang, Studying for master’s degree, Department of Anesthesiology, First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30770689, 81171281; the Natural Science Foundation of Hunan Province, No. 10JJ2009; the General Program of Bureau of Science and Technology of Hengyang City of Hunan Province, No. 2012KJ41

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摘要：

文章快速阅读：

 

文题释义：
新颖物体识别：是利用啮齿类动物先天对新物体有探究倾向的原理而建立的一种学习记忆测试模型；其基本实验操作就是首先让大鼠或小鼠在一个局限空间内探究2个等同或不同的小物体，一段时间之后用一个新颖物体取代其中一个已经探究过的物体(熟悉的物体)之后再让大鼠或小鼠探究一段时间，记录分析大鼠或小鼠对新颖物体与熟悉物体探究的频率或时间差值从而对其记忆能力进行评估。
记忆再巩固：即记忆的激活导致原有巩固的记忆进入一段不稳定的时期，需要特殊的过程来恢复到稳定的状态。这与其他新习得的记忆所要经历的从不稳定到稳定状态的记忆过程相似。
 
背景：长时程记忆形成通常包括获得、巩固、再巩固等多个环节，再巩固过程对于新获得的记忆转为稳定记忆十分重要。在学习记忆过程中，应激是一种重要的环境因素；而在应激过程中，皮质酮是发挥重要作用的一种激素。目前，关于皮质酮对新颖物体识别记忆再巩固的影响未见相关研究报道。因此检测皮质酮对大鼠新颖物体识别记忆再巩固过程的影响具有十分重要的意义。
目的：分析皮质酮对大鼠新颖物体识别记忆再巩固的影响。
方法：①大鼠在再激活后立即腹腔注射皮质酮(0.1，1及3 mg/kg)，以大鼠在测试阶段对新旧物体探究时间的辨别指数作为记忆评价指标；②再激活后6 h腹腔注射皮质酮(3 mg/kg)，以大鼠在测试阶段对新旧物体探究时间的辨别指数作为记忆评价指标；③无再激活经历时腹腔注射皮质酮(3 mg/kg)，以大鼠在测试阶段对新旧物体探究时间的辨别指数作为记忆评价指标。
结果与结论：再激活后立即注射皮质酮(3 mg/kg)显著性降低大鼠辨别指数，再激活后6 h或无再激活经历时注射皮质酮对大鼠辨别指数无影响。提示再激活后立即注射皮质酮损害大鼠新颖物体识别记忆再巩固，该损害效应依赖于再激活经历及再激活后特定时间窗。
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程
ORCID: 0000-0001-5850-1586(周梅芳)

关键词: 实验动物, 神经损伤与修复动物模型, 皮质酮, 再巩固, 识别记忆, 应激, 辨别指数, 再激活, 学习, 国家自然科学基金

Abstract:

BACKGROUND: The formation of long-term memory includes acquisition, consolidation and reconsolidation. Reconsolidation is very important for the new memory transforming into stable memory. Stress is an important environmental factor in the process of learning and memory. Corticosterone is very important for stress response. At present, research about the impact of corticosterone on reconsolidation of novel object recognition memory is less. Thus, it is very important to test the impact of corticosterone on reconsolidation of novel object recognition memory in rats.

OBJECTIVE: To analyze the effects of corticosterone on the reconsolidation of novel object recognition memory in rats.
METHODS: (1) The rats were intraperitoneally injected with corticosterone (0.1, 1 and 3 mg/kg) immediately after reactivation. The discrimination index was used to assess memory performance and calculated as the difference in time exploring the novel and familiar objects. (2) The rats were intraperitoneally injected with corticosterone (3 mg/kg) 6 hours after reactivation. The discrimination index was used to assess memory performance and calculated as the difference in time exploring the novel and familiar objects. (3) The rats were intraperitoneally injected with corticosterone 3 mg/kg without reactivation. The discrimination index was used to assess memory performance and calculated as the difference in time exploring the novel and familiar objects.
RESULTS AND CONCLUSION: Immediate injection of corticosterone 3 mg/kg after reactivation significantly decreased the discrimination index. At 6 hours after reactivation or without reactivation, corticosterone administration did not impact the discrimination index. These results confirmed that corticosterone administration immediately after reactivation impairs the reconsolidation of novel object recognition memory. The damage effect depends on the reactivation experience and the specific time window after reactivation. 
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Corticosterone, Learning, Memory, Tissue Engineering