中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (18): 2628-2633.doi: 10.3969/j.issn.2095-4344.2016.18.007

• 周围神经损伤动物模型 Animal models of peripheral nerve injury • 上一篇    下一篇

脑及脊髓冲击震动损伤模型生化指标的变化

朱亚鹏1，郭延岭2，常 祺2   

  1. 1解放军第四军医大学第一附属医院骨科医院，骨科研究所，陕西省西安市  710032；2解放军150中心医院，河南省洛阳市  471000
  • 收稿日期:2016-02-03 出版日期:2016-04-29 发布日期:2016-04-29
  • 通讯作者: 常祺，博士，硕士生导师，解放军150中心医院，河南省洛阳市 471000
  • 作者简介:朱亚鹏，男，1989年生，河南省南阳市人，汉族，解放军第四军医大学在读硕士，主要从事四肢创伤骨折及修复方面的研究。
  • 基金资助:

    全军医学科技青年培育项目(13QNP033)

Changes of biochemical indexes in the brain and spinal cord after shock and vibration damage

Zhu Ya-peng1, Guo Yan-ling2, Chang Qi2   

  1. 1Institute of Orthopedics, Orthopedic Hospital, First Affiliated Hospital, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China; 2PLA 150 Central Hospital, Luoyang 471000, Henan Province, China
  • Received:2016-02-03 Online:2016-04-29 Published:2016-04-29
  • Contact: Chang Qi, M.D., Master’s supervisor, PLA 150 Central Hospital, Luoyang 471000, Henan Province, China
  • About author:Zhu Ya-peng, Studying for master’s degree, Institute of Orthopedics, Orthopedic Hospital, First Affiliated Hospital, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
  • Supported by:

    the Army Medical Technology Youth Development Project, No. 13QNP033

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摘要：

文题释义：

 

文题释义：
冲击伤：是冲击波超压和负压引起的损伤，主要造成含气器官如肺脏、听器、胃肠道的损害，超强压还可以造成内脏破裂和肋骨骨折等，但一般较少造成体表损伤。单纯冲击伤致伤时，体表多完好无损，但常有不同程度的内脏损伤，表现为外轻内重的特点。
生化指标：全套主要包括肝功能(总蛋白、白蛋白、球蛋白、白球比，总胆红素、直接、间接胆红素，转氨酶)；血脂(总胆固醇，三酰甘油，高、低密度脂蛋白，载脂蛋白)；空腹血糖；肾功能(肌酐、尿素氮)；尿酸；乳酸脱氢酶；肌酸肌酶等。
 
背景：高能震动易于损伤机体非空腔器官且损伤效应显著，但目前针对高能震动致伤过程的研究较少。
目的：了解高能震动致伤后动物机体生理生化及病理改变。
方法：将32只中华田园犬随机分为4组，轻度震伤组、中度震伤组及重度震伤组分别实施700，1 000，2 100 m/s²的冲击震动，对照组为正常对照。于震后14 d内检测血清血清K+、Ca2+、Zn2+、S100β、神经元特异性烯醇化酶浓度，并进行脊髓及颅脑组织免疫组织化学染色观察。
结果与结论：震伤3组血清K+、Ca2+、Zn2+浓度呈现规律性变化，震后即时无明显变化，K+浓度于震后0.5 d降到最低，Ca2+浓度至震后1 d降到最低，Zn2+浓度于震后0.5 d或1d降到最低，此后均逐渐增加，至14 d时恢复正常水平；震伤3组血清神经元特异性烯醇化酶、S100β均于震后0.5 d开始增加，于震后1 d达到最高值，此后均逐渐降低，至14 d时恢复至正常或偏高水平。震伤3组脊髓及颅脑均出现接触部位及对冲部位的出血点，震后即时处死时出血程度较14 d时明显，脊髓及颅脑损伤部位S100β、胶质纤维酸性蛋白、神经元特异性烯醇化酶增多。
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程
ORCID: 0000-0002-4550-8911(常祺)

关键词: 实验动物, 神经损伤与修复动物模型, 高能震动, 瞬时震动, 爆震伤, 神经系统, 损伤

Abstract:

BACKGROUND: High-energy vibration is easy to damage non-cavity organs of the body and the damage effect is remarkable, but few studies concern the process of high-energy vibration-induced injury. 

OBJECTIVE: To understand physiological, biochemical and pathological changes of animal bodies after high-energy vibration-induced injury.
METHODS: A total of 32 dogs were randomly divided into four groups. Dogs in the mild vibration injury group, moderate vibration injury group and severe vibration injury group received 700, 1 000, and 2 100 m/s² vibration. Dogs in the control group were considered as normal controls. Within 14 days after vibration, serum K+, Ca2+, Zn2+, S100β, and neuron specific enolase concentrations were detected. Immunohistochemical staining was observed in the spinal cord and the brain. RESULTS AND CONCLUSION: Serum K+, Ca2+, Zn2+ concentrations showed a regular change in the three vibration injury group, and no evident change was found immediately after vibration injury. K+ concentrations reached a minimum at 0.5 day after vibration injury. Ca2+ concentrations reached a minimum at 1 day after vibration injury. Zn2+ concentrations reached a minimum at 0.5 or 1 day. Above concentrations gradually increased and became normal at 14 days. Serum neuron specific enolase and S100β expression increased at 0.5 day after vibration in the three vibration injury groups, peaked at 1 day, gradually diminished, and recovered to a normal level or higher level at 14 days. In the three vibration groups, bleeding point of contact position and hedge position could be found in the spinal cord and brain. The degree of bleeding was more significant when killed instantly after vibration compared with that at 14 days. S100β, glial fibrillary acidic protein and neuron specific enolase expression increased in the spinal cord and brain.
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Animals, Laboratory, High-Energy Shock Waves, Central Nervous System, Blast Injuries, Tissue Engineering