中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (18): 2622-2627.doi: 10.3969/j.issn.2095-4344.2016.18.006

• 周围神经损伤动物模型 Animal models of peripheral nerve injury • 上一篇    下一篇

脊髓康对脊髓损伤大鼠Nogo-66受体NgR表达的影响

郭 杨1，马 勇1，2，冯 诚1，潘娅岚1，黄桂成1   

  1. 1南京中医药大学骨伤研究所、骨伤修复与重建新技术实验室，江苏省南京市 210023；2南京中医药大学附属医院骨伤科，江苏省南京市  210029
  • 收稿日期:2016-02-05 出版日期:2016-04-29 发布日期:2016-04-29
  • 通讯作者: 马勇，教授，博士生导师，南京中医药大学骨伤研究所，江苏省南京市 210023；南京中医药大学附属医院骨伤科，江苏省南京市 210029?
  • 作者简介:郭杨，博士，助理研究员，主要从事为中医药防治骨关节病的研究。
  • 基金资助:

    江苏省高校自然科学研究面上项目(15KJD360001)；国家自然科学基金项目(81573997)；江苏省普通高校研究生科研创新计划项目(CXZZ12_0609)；江苏省自然科学基金资助项目(BK2011180)

Effect of Jisuikang on Nogo-66 receptor NgR expression in rats with spinal cord injury

Guo Yang1, Ma Yong1, 2, Feng Cheng1, Pan Ya-lan1, Huang Gui-cheng1   

  1. 1Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China; 2Department of Traumatology & Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • Received:2016-02-05 Online:2016-04-29 Published:2016-04-29
  • Contact: Ma Yong, Professor, Doctoral supervisor, Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China; Department of Traumatology & Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • About author:Guo Yang, M.D., Assistant researcher, Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China
  • Supported by:

    the Natural Science Research Program Project of Jiangsu Province Universities, No. 15KJD360001; the National Natural Science Foundation of China, No.81573997; the Postgraduate Science Research and Innovation Project of Jiangsu Colleges, No. CXZZ12_0609; the Jiangsu Province Natural Science Foundation, No. BK2011180

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678

被引次数

3

摘要：

文题释义：

 

文题释义：
脊髓损伤：是指由于外界直接或间接因素导致脊髓损伤，在损害的相应节段出现各种运动、感觉和括约肌功能障碍，肌张力异常及病理反射等的相应改变。脊髓损伤的程度和临床表现取决于原发性损伤的部位和性质。脊髓损伤可分为原发性脊髓损伤与继发性脊髓损伤。前者是指外力直接或间接作用于脊髓所造成的损伤。后者是指外力所造成的脊髓水肿、椎管内小血管出血形成血肿、压缩性骨折以及破碎的椎间盘组织等形成脊髓压迫所造成的脊髓的进一步损害。

Nogo受体NgR：是Nogo-A发挥作用的受体，是一种糖基磷脂酰肌醇锚定膜蛋白，能与细胞外结构域Nogo-66结合，并被激活，激活的NgR启动神经细胞内的信号转导通路抑制轴突再生和结构的重塑性。
 

背景：研究发现，脊髓损伤后髓鞘相关抑制分子的产生是影响轴突再生微环境的重要原因。中医药具有多因素、多靶点的优点，正逐步成为改善神经再生微环境的研究热点。
目的：探索中药脊髓康对脊髓损伤后Nogo-66受体NgR表达的影响。
方法：144只大鼠随机等分为6组，假手术组、模型组、强的松组、脊髓康高、中、低剂量组，每组24只。后5组以改良Allen’s法建立脊髓损伤动物模型。强的松组大鼠每天给予0.06 g/kg醋酸泼尼松灌胃，1次/d。脊髓康低中高剂量组大鼠每天给予12.5，25，50 g/kg脊髓康灌胃，1次/d。假手术组和模型组大鼠每天给予20 mL生理盐水灌胃，1次/d。各组动物均连续给药至动物处死。
结果与结论：与模型组相比，强的松组及脊髓康低中剂量组大鼠脊髓组织中NgR蛋白及mRNA表达水平均显著降低。提示脊髓康可促进大鼠脊髓损伤后神经功能的恢复，可有效抑制脊髓损伤区NgR蛋白的表达，从而抑制不利于神经再生的因素，进一步改善神经再生的微环境。

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程
ORCID: 0000-0002-8518-6160(郭杨)

关键词: 实验动物, 神经损伤与修复动物模型, 脊髓康, 脊髓损伤, NgR, 轴突生长抑制因子, 神经再生, 微环境, 中医药, 肾督同治, 国家自然科学基金

Abstract:

BACKGROUND: Myelin sheath related inhibitors have been found to have great impact on microenvironment of axon regeneration. Traditional Chinese medicine is gradually becoming a research hotspot on improving microenvironment of nerve regeneration with its advantage on multiple factors and targets.

OBJECTIVE: To clarify the effect of Jisuikang on Nogo-66 receptor NgR expression after spinal cord injury. 
METHODS: 144 rats were randomly divided into six groups: sham surgery group, model group, prednisone group, high-, moderate- and low-dose Jisuikang groups (n=24). Animal models of spinal cord injury were established by the modified Allen’s method in the later five groups. Rats in the prednisone group were daily given 0.06 g/kg prednisone acetate by lavage, once a day. Rats in the high-, moderate- and low-dose Jisuikang groups were daily intragastrically given 12.5, 25 and 50 g/kg Jisuikang, once a day. Rats in the sham surgery and model groups were intragastrically daily given 20 mL of saline, once a day. Rats in each group were administered drugs until death.
RESULTS AND CONCLUSION: Compared with the model group, NgR protein and mRNA expression levels were significantly lower in the prednisone, moderate-and low-dose Jisuikang groups. These data suggested that Jisuikang can improve the recovery of neurological function after spinal cord injury and effectively inhibit NgR protein expression at the site of injury so as to suppress the microenvironment factors harmful to nerve regeneration and further improve the microenvironment of nerve regeneration.
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Drugs, Chinese Herbal, Spinal Cord Injuries, Axons, Tissue Engineering