中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (15): 2184-2189.doi: 10.3969/j.issn.2095-4344.2016.15.008

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

甘草黄酮对运动大鼠骨骼肌Perilipin mRNA及蛋白表达的影响

王成科1，莫伟彬2，3，潘昌红2，张 蒙2，黄 东2   

  1. 1广西民族师范学院体育系，广西壮族自治区崇左市  532200；2广西师范大学体育学院，广西壮族自治区桂林市  541004；3药用资源化学与药物分子工程教育部重点实验室，广西壮族自治区桂林市  541004
  • 收稿日期:2016-02-04 出版日期:2016-04-08 发布日期:2016-04-08
  • 通讯作者: 莫伟彬，硕士，实验师，广西师范大学体育学院, 广西壮族自治区桂林市 541004
  • 作者简介:王成科，男，1983年生，湖南省祁东县人，汉族，2010年广西师范大学毕业，硕士，讲师，主要从事运动生理学、运动生物化学和药理学的研究。
  • 基金资助:

    国家自然科学基金项目(31460232，21562006)；广西自然科学资金面上项目(2015GXNSFAA139186)；“药用资源化学与药物分子工程教育部重点实验室”开放课题 (CMEMR2012-B04).

Effects of Glycyrrhiza flavonoids on expression of perilipin mRNA and protein in skeletal muscle of rats after exhaustive exercise

Wang Cheng-ke1, Mo Wei-bin2, 3, Pan Chang-hong2, Zhang Meng2, Huang Dong2   

  1. 1Department of Sports, Guangxi Normal University for Nationalities, Chongzuo 532200, Guangxi Zhuang Autonomous Region, China; 2Sport School of Guangxi Normal University, Guilin 541004, Guangxi Zhuang Autonomous Region, China; 3State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guilin 541004, Guangxi Zhuang Autonomous Region, China
  • Received:2016-02-04 Online:2016-04-08 Published:2016-04-08
  • Contact: Mo Wei-bin, Master, Experimentalist, Sports School of Guangxi Normal University, Guilin 541004, Guangxi Zhuang Autonomous Region, China; State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guilin 541004, Guangxi Zhuang Autonomous Region, China
  • About author:Wang Cheng-ke, Master, Lecturer, Department of Sports, Guangxi Normal University for Nationalities, Chongzuo 532200, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31460232, 21562006; the Natural Science Foundation of Guangxi, No. 2015GXNSFAA139186; State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China Open Projects Fund, No. CMEMR2012-B04

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摘要：

文章快速阅读：

 

文题释义：
甘草黄酮：黄酮类化合物，对肌肉组织具有抗自由基能力、保护肌肉的损伤、抗炎、减轻脂质过氧化的反应和调控脂肪组织方面的作用。
围脂滴蛋白：是一类细胞结构，同属于PAT蛋白家族，根据基因的编码不同，分为peri A、peri B和peri C等3种亚型，具有调控机体各脂肪组织的储存、释放和参与机体代谢的功能，其表达主要存在于腓肠肌、比目鱼肌、趾长伸肌、小肠、肝和心肌等组织中。


 

背景：甘草黄酮对肌肉组织具有抗自由基能力、保护肌肉的损伤、抗炎、减轻脂质过氧化的反应和调控脂肪组织方面的作用。
目的：通过研究甘草黄酮干预作用下运动性大鼠骨骼肌围脂滴蛋白mRNA及蛋白表达的作用及可能机制，观察甘草黄酮对调控不同的脂肪组织的储存、释放和参与机体能量代谢的能力。
方法：SD雄性大鼠50只，每组10只，随机分为安静对照组、运动对照组、运动+甘草黄酮低、中、高剂量组。各组灌胃不同剂量甘草黄酮，对照组灌胃等量生理盐水，6周力竭运动训练后，处死大鼠。根据试剂盒的方法测定骨骼肌(腓肠肌和比目鱼肌)围脂滴蛋白 mRNA及蛋白表达水平。
结果与结论：运动对照组腓肠肌围脂滴蛋白mRNA表达水平高于安静对照组(P < 0.01)；在甘草黄酮各组腓肠肌围脂滴蛋白 mRNA表达水平高于安静对照组(P < 0.05或P < 0.01)；运动+甘草黄酮中、高剂量组围脂滴蛋白蛋白表达高于安静对照组(P < 0.05)。运动+甘草黄酮中剂量组比目鱼肌围脂滴蛋白 mRNA表达水平高于安静对照和运动对照组(P < 0.05)；同组比较时，运动对照组和在甘草黄酮各组比目鱼肌围脂滴蛋白 mRNA及蛋白表达均低于腓肠肌(P < 0.01)。结果提示甘草黄酮可能有利于改善骨骼肌纤维的有氧代谢能力和调控脂肪的酯解通路。


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0001-5864-8457(王成科)

关键词: 组织构建, 组织工程, 甘草黄酮, 运动, 大鼠, 围脂滴蛋白, 腓肠肌, 国家自然科学基金

Abstract:

BACKGROUND: Glycyrrhiza flavonoids can protect muscle tissues against oxidative and inflammatory injuries.
OBJECTIVE: To explore the effects of Glycyrrhiza flavonoids on energy storing and release of adipose tissue by studying expressions of perilipin mRNA and protein in skeletal muscle tissues of rats after exhaustive exercise.
METHODS: Fifty male healthy Sprague-Dawley rats were used and equally randomized into five groups: quiet control, exercise alone (intragastric administration with saline), exercise combined with low-, moderate-, high-dose Glycyrrhiza flavonoids (intragastric administration with Glycyrrhiza flavonoids) groups, respectively. Perilipin mRNA and protein expressions in skeletal muscle tissues containing gastrocnemius muscle and musculi soleus were determined at 6 weeks after exhaustive exercise.
RESULTS AND CONCLUSION: Expression of perilipin mRNA in rat gastrocnemius muscle in quiet control group was significantly decreased compared with exercise alone and all combined intervention groups (P < 0.05 or P < 0.01). Protein expression of perilipin in exercise combined with moderate- or high-dose glycyrrhiza flavonoids group was significantly increased compared with quiet control group (P < 0.05). Expression of perilipin mRNA in rat musculi soleus was significantly decreased compared with exercise combined with moderate-dose glycyrrhiza flavonoids group (P < 0.05). These findings confirm that Glycyrrhiza flavonoids are benefit for improvement of aerobic metabolism capacity of gastrocnemius muscle through regulating lipolysis pathway.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Flavones, Muscle Fibers, Skeletal, Adipose Tissue