中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (3): 330-335.doi: 10.3969/j.issn.2095-4344.2016.03.005

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

数字化珊瑚羟基磷灰石人工骨支架的体外降解性能

林 山1，黄晓梅2，3，芮 钢1，尹庆水4   

  1. 1厦门大学附属第一医院骨科，福建省厦门市  361003；2漳州职业技术学院食品与生物工程系，福建省漳州市  363000；3中国科学院城市环境研究所，城市生态健康与环境安全研究中心，福建省厦门市  361021；4解放军广州军区广州总医院，全军创伤骨科中心，广东省广州市  510010
  • 收稿日期:2015-10-26 出版日期:2016-01-15 发布日期:2016-01-15
  • 作者简介:林山，男，1981年生，博士，主治医师，主要从事数字骨科与生物骨科材料及运动医学研究。
  • 基金资助:
    福建省教育厅科技项目(JA12416)；福建省自然科学基金青年创新项目(2013D013)；漳州职业技术学院院级科研项目(ZZY1204)

Degradation performance of the digital coralline hydroxyapatite artificial bone scaffold in vitro

Lin Shan1, Huang Xiao-mei2, 3, Rui Gang1, Yin Qing-shui4    

  1. 1Department of Orthopedics, the First Affiliated Hospital of Xiamen University, Xiamen 361003, Fujian Province, China; 2Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, Fujian Province, China; 3Research Center of Urban Ecological Health and Environmental Safety, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, Fujian Province, China; 4Orthopedic Trauma Center of PLA, Guangzhou General Hospital of Guangzhou Military Region, Guangzhou 510010, Guangdong Province, China
  • Received:2015-10-26 Online:2016-01-15 Published:2016-01-15
  • Contact: Lin Shan, Department of Orthopedics, the First Affiliated Hospital of Xiamen University, Xiamen 361003, Fujian Province, China
  • About author:Lin Shan, M.D., Attending physician, Department of Orthopedics, the First Affiliated Hospital of Xiamen University, Xiamen 361003, Fujian Province, China
  • Supported by:

    the Science and Technology Project of Fujian Provincial Department of Education of China, No. JA12416; the Youth Innovation Project of Natural Science Foundation of Fujian Province, China, No. 2013D013; School-Level Research Project of Zhangzhou Institute of Technology, No. ZZY1204

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356

被引次数

5

摘要：

文章快速阅读：

文题释义：

珊瑚羟基磷灰石：是由天然珊瑚经过“水热反应”转化生成的，目前已被广泛应用于骨科、口腔科及眼科领域。珊瑚羟基磷灰石的主要成分是羟基灰石和碳酸钙。羟基磷灰石在体内几乎不发生降解，据文献报道，完全转化的珊瑚羟基磷灰石2年降解率为8%。过低的降解度导致新骨形成过慢，同时形成的新骨生物力学性能与正常骨组织存在较大差距，不能满足正常的骨生理功能。

生物可降解：指材料在生物体内通过溶解、酶解、细胞吞噬等作用，在组织长入的过程中不断从体内排出，修复后的组织完全替代植入材料的位置，而材料在体内不存在残留的性质。生物降解的研究内容包括生物自身所具有的降解能力，有机物降解难易的规律，水溶性和非水溶性有机物生物降解的机理，以及生物降解途径等。

 

背景：前期实验成功制备了数字化珊瑚羟基磷灰石人工骨支架材料，并已证实其具有良好的理化性能和生物相容性。
目的：评价数字化珊瑚羟基磷灰石人工骨的体外降解性能。
方法：将珊瑚羟基磷灰石与左旋-聚乳酸分别以3∶1和4∶1的质量比混合，制备数字化珊瑚羟基磷灰石人工骨支架材料试件。将两种数字化人工骨支架材料、珊瑚羟基磷灰石及左旋-聚乳酸分别置于初始pH值为7.4的50 mL模拟体液中，在37 ℃恒温箱中降解16周，动态观察溶液pH值、钙及磷离子浓度，以及材料降解率、抗压强度及微观结构变化。

结果与结论：降解16周时，两种数字化人工骨组的pH值维持在7.34-7.36，高于左旋-聚乳酸组(P < 0.01)，低于珊瑚羟基磷灰石组(P < 0.01)；两种数字化人工骨组的钙离子浓度高于珊瑚羟基磷灰石组(P < 0.01)，磷离子浓度低于珊瑚羟基磷灰石组(P < 0.01)；两种数字化人工骨组的降解率低于珊瑚羟基磷灰石组(P < 0.01)，高于左旋-聚乳酸组(P < 0.01)；抗压强度顺序：珊瑚羟基磷灰石组> 3∶1数字化人工骨组>左旋-聚乳酸组> 4∶1数字化人工骨组；两种数字化人工骨微孔结构增多，孔隙率升高，孔径增大，说明数字化珊瑚羟基磷灰石人工骨支架具有良好的降解性能。 

ORCID: 0000-0003-4037-7411(林山)

关键词: 生物材料, 骨生物材料, 珊瑚羟基磷灰石, 左旋-聚乳酸, 快速成形, 骨组织工程, 生物降解性, 福建省自然科学基金

Abstract:

BACKGROUND: We have successfully prepared the digital coralline hydroxyapatite artificial bone scaffold in previous experiments, and it has good physicochemical properties and biocompatibility.
OBJECTIVE: To evaluate the in vitro degradation performance of the digital coralline hydroxyapatite artificial bone.
METHODS: We used the mixtures of coralline hydroxyapatite and L-polylactic acid at the mass ratio of 3:1 and 4:1 as raw materials to prepare the digital coralline hydroxyapatite artificial bone scafflold specimens, and then they were immersed in the 50 mL stimulated body fluid with the initial pH value of 7.4 in an incubator at 37 ℃ for degradation. After 16 weeks of degradation, the pH value, calcium and phosphate ion concentration, degradation rate, compressive strength and changes of microstructure were dynamically observed.
RESULTS AND CONCLUSION: At the 16th weeks of degradation, the pH values in the two kinds of digital artificial bone groups maintained at 7.34-7.36, which were higher than that in the L-polylactic acid group (P < 0.01), and lower than that in the coralline hydroxyapatite group (P < 0.01). The calcium ion concentrations in the two kinds of digital artificial bone groups were higher than that in the coralline hydroxyapatite group (P < 0.01), and the phosphorus ion concentrations were lower than that in the coralline hydroxyapatite group (P < 0.01). The degradation rates in the two kinds of digital artificial bone groups were lower than that in the coralline hydroxyapatite group (P < 0.01), and higher than that in the L-polylactic acid group (P < 0.01). The order of the compressive strength was as follows: coralline hydroxyapatite group > 3:1 digital artificial bone group > L-polylactic acid group > 4:1 digital artificial bone group. The cellular structure, porosity and pore size in the two kinds of digital artificial bone groups were all increased. These results show that the prepared digital coralline hydroxyapatite artificial bone scaffold has good degradation propertyies.