中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (2): 197-201.doi: 10.3969/j.issn.2095-4344.2016.02.008

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

上、下坡跑台训练膝骨关节炎模型大鼠关节软骨及炎症因子的变化

王文胜，陈 伟，沈堡垒，许 波，张 俐，江 征   

  1. 福建中医药大学，福建省福州市 350122
  • 收稿日期:2015-11-07 出版日期:2016-01-08 发布日期:2016-01-08
  • 通讯作者: 江征，博士，副教授，硕士生导师，福建中医药大学，福建省福州市 350122
  • 作者简介:王文胜，男，1988年生，河南省淮阳县人，汉族，福建中医药大学在读硕士，主要从事中医药在骨与关节损伤中应用方向的研究。 并列第一作者：陈伟，福建中医药大学，福建省福州市 350122
  • 基金资助:
    福建省教育厅A类科技项目(JA12167)

Effect of uphill or downhill running training on articular cartilage degeneration and inflammatory responses of knee osteoarthritis rats

Wang Wen-sheng, Chen Wei, Shen Bao-lei, Xu Bo, Zhang Li, Jiang Zheng   

  1. Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
  • Received:2015-11-07 Online:2016-01-08 Published:2016-01-08
  • Contact: Jiang Zheng, M.D., Associate professor, Master’s supervisor, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
  • About author:Wang Wen-sheng, Studying for master’s degree, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China Chen Wei, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China Wang Wen-sheng and Chen Wei contributed equally to this work.
  • Supported by:

    A Project of Science and Technology Education Department of Fujian Province, No. JA12167

PDF

542

被引次数

4

摘要：

文章快速阅读： 

文题释义：
膝骨关节炎的国际诊断标准：
(1)临床诊断标准：大多数时间内有膝痛。②有骨摩擦音。③晨僵< 30 min。④年龄≥ 38岁。⑤膝检查示骨性肥大。满足①②③④或①②⑤或①④⑤者可诊断为膝骨关节炎。
(2)临床、实验室和放射学诊断标准：①大多数时间内有膝痛。②X射线片示关节边缘有骨赘。③关节液检查符合骨关节炎。④年龄≥ 40岁。⑤晨僵< 30 min。⑥关节活动时有骨响声。满足①②或①③⑤⑥或①④⑤⑥者可诊断为膝骨关节炎。
跑台：即小动物跑步训练台，其原理类似人类的跑步机，但其结构更简单，由底座、发动机、轴承、履带、分割栅栏、电脑六部分组成的履带式6道跑台。

 

背景：不同的训练方式对原发性膝骨关节炎模型的建立都会产生不同的影响。
目的：对比观察上坡和下坡跑台训练在诱导大鼠膝骨关节炎模型过程中对膝关节软骨变性及炎症反应的作用。
方法：SD雌性大鼠108只，随机等分为6组：空白组、上坡跑步、下坡跑步组、去卵巢模型组、模型+上坡跑步组和模型+下坡跑步组。空白组大鼠常规饲养不做任何处理；上坡跑步组大鼠常规饲养并进行+15°跑台训练；下坡跑步组大鼠常规饲养并进行-15°跑台训练；模型组大鼠行双侧卵巢切除后常规饲养；模型+上坡跑步组大鼠去卵巢后进行+15°跑台训练；模型+下坡跑步组去卵巢后进行-15°跑台训练。跑台训练的频率均为28 m/min，60 min/d，6 d/周。

结果与结论：造模4周时，模型+上坡跑步组大鼠Mankin评分明显较另5组增高，已达软骨损伤标准，尿液中Ⅱ型胶原羧基端肽浓度已过峰值期；6 周时模型+下坡跑步组大鼠Mankin评分达到了软骨损伤的标准，较模型+上坡跑步组低，模型+上坡跑步组和模型+下坡跑步组大鼠肿瘤坏死因子α、白细胞介素1β质量浓度均较其余4组增高；8 周时模型+上坡跑步组和模型+下坡跑步组大鼠Mankin评分及关节液肿瘤坏死因子α、白细胞介素1β质量浓度进一步增加，其中模型+上坡跑步组更为严重。结果提示去势后联合疲劳性跑台训练，上坡和下坡训练均可造成大鼠关节软骨的损伤及关节局部炎症反应，上坡训练与下坡训练相比，软骨损伤出现较早，效率更高。 

 ORCID: 0000-0002-2067-7954(江征)

关键词: 组织构建, 软骨组织工程, 膝骨关节炎, 动物模型, 跑台训练, 去卵巢, 软骨, 关节液, 尿液

Abstract:

BACKGROUND: Different training methods have different effects on primary knee osteoarthritis.
OBJECTIVE: To compare the effects of uphill or downhill running on articular cartilage degeneration and inflammatory responses of knee osteoarthritis rats.
 
METHODS: 108 female Sprague-Dawley rats were randomly divided into six groups: normal control group, ovariectomized group, uphill running group (run & up), downhill running group (run & down), uphill running model group (model & up), downhill running model group (model & down). The rats in the normal control group were raised routinely with no treatment; rats in the ovariectomized group were ovarientomized and raised in routine way; rats in the run & up group and run & down group were subjected to uphill running training on the slop +15° or downhill running training on the slop -15°; rats in the model & up group and model & down group were subjected to uphill running training on the slop +15°or downhill running training on the slop -15° after ovarientomized. Training programs were as follows: 28 m/min, 60 minutes per day, 6 days per week.
RESULTS AND CONCLUSION: Compared with the other five groups, in the model & up group, the Mankin’s score was significantly increased, indicating that articular cartilage degeneration occurred, and the peak of carboxy-terminal telopeptide of type II collagen contained in the urine had passed, at 4 weeks after modeling. At 6 weeks, the Mankin’s score of the model & down group was increased to reach the degeneration standard, but it was still lower than that of the model & up group; the expression levels of tumor necrosis factor-α and interleukin-1β in the synovial fluid were significantly higher in the model & up and model & down groups than the other four groups. At 8 weeks, the Mankin’s score and the expression levels of tumor necrosis factor-α and interleukin-1β were further increased in the model & up and model & down groups, especially in the model & up group. These results indicate that treadmill running exercise after ovariectomized can result in articular cartilage injury and local inflammatory responses; compared with the downhill running training, the uphill running training can cause cartilage injury earlier and more efficiently.