中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (46): 7381-7385.doi: 10.3969/j.issn.2095-4344.2015.46.001

• 骨组织构建 bone tissue construction •    下一篇

骨折愈合过程中的反复短暂缺血：早期microCT观察

王 东，刘 洋，赵彦瑞，单 磊，周君琳   

  1. 首都医科大学附属北京朝阳医院骨科，北京市 100020
  • 收稿日期:2015-10-13 出版日期:2015-11-12 发布日期:2015-11-12
  • 通讯作者: 周君琳，博士，主任医师，教授，博士生导师，首都医科大学附属北京朝阳医院骨科，北京市 100020
  • 作者简介:王东，男，1988年生，北京市人，汉族，2015年首都医科大学毕业，硕士，医师，主要从事创伤骨科研究。
  • 基金资助:

    北京市自然科学基金资助项目(7152061)

Repeated transient ischemia during fracture healing: a micro-CT observation

Wang Dong, Liu Yang, Zhao Yan-rui, Shan Lei, Zhou Jun-lin   

  1. Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
  • Received:2015-10-13 Online:2015-11-12 Published:2015-11-12
  • Contact: Zhou Jun-lin, M.D., Chief physician, Professor, Doctoral supervisor, Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
  • About author:Wang Dong, Master, Physician, Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
  • Supported by:

    Beijing Natural Science Foundation, No. 7152061

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摘要：

背景：有研究表明阻断静脉回流可以促进骨折愈合，但尚无有关于对患肢行反复短暂缺血即完全阻断血流促进骨折愈合的研究。
目的：基于microCT技术，观察反复短暂缺血对骨折愈合早期的促进作用。
方法：48只Wistar大鼠制作右侧胫骨中段闭合骨折模型后克氏针固定骨折，随机分为4组，24 h组、48 h组、72 h组和对照组。大鼠患肢大腿根部上止血带，充气10 min后松解10 min，3次循环制作1回缺血处理。大鼠骨折固定后24 h行缺血处理1回，之后，24 h组每隔24 h、48 h组每隔48 h、72 h组每隔72 h行1回缺血处理。对照组于骨折固定后每隔24 h右下肢上止血带1 h，但不予以充气。4组大鼠于制作骨折模型后第2周处死，截取右侧胫骨，取出克氏针行右侧胫骨microCT检查。
结果与结论：24 h组ROI区域骨小梁体积、总体积均高于其他3组(P < 0.05)，24 h组大鼠组织矿物密度、骨痂组织矿物密度均低于其他3组(P < 0.05)。4组间骨体积分数差异无显著性意义(P > 0.05)。结果提示，骨折后2周内，反复短暂缺血处理可以促进骨折愈合。可能与反复短暂缺血处理可以刺激局部分泌生长因子，加快血管重建以及成骨细胞增殖有关。
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 组织构建, 骨组织工程, 反复短暂缺血处理, 骨折愈合, 早期, microCT观察, 北京市自然科学基金

Abstract:

BACKGROUND: Some studies have shown that blocking the venous return can promote fracture healing but there is no study about the effect of repeated transient ischemia on promoting fracture healing.
OBJECTIVE: To observe the promoting effect of repeated transient ischemia on early fracture healing based on micro-CT technique.
METHODS: Forty-eight Wistar rats were used to make animal models of right tibial midpiece closed fractures followed by K-wire fixation. Rats were randomly divided into four groups: 24-hour group, 48-hour group, 72-hour group and control group. Tourniquet was used on the root of the affected thigh, inflated for 10 minutes, and then released for 10 minutes. Three above-mentioned cycles were taken as an ischemic treatment. At 24 hours after fracture fixation, the ischemic treatment was first carried out, and after that, it was done every 24 hours in the 24-hour group, every 48 hours in the 48-hour group and every 72 hours in the 72-hour group. In the control group, the tourniquet with no inflation was used once on the right lower limb every 24 hours after fixation. All the rats were killed at 2 weeks after modeling to take out the right tibia with removal of K-wires for micro-CT observation.
RESULTS AND CONCLUSION: The trabecular bone volume and total volume in the regions of interest were significantly higher in the 24-hour group than the other three groups (P < 0.05), but the tissue mineral density and callus tissue mineral density were significantly lower in the 24-hour group than the other three groups (P < 0.05). There was no significant statistical difference in the bone volume fraction among the four groups (P > 0.05). These findings indicate that within 2 weeks after fractures, the repeated transient ischemia can promote fracture healing, which may be related to the improvement in the secretion of growth factor, revascularization and osteoblast proliferation induced by repeated transient ischemia.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Fractures, Bone, Fracture Healing, Ischemic Preconditioning, Tissue Engineering