中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (21): 3355-3360.doi: 10.3969/j.issn.2095-4344.2015.21.014

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜的制备及相关性能评价

马  遥，储顺礼，孙  悦，马珊珊，李  雪，张天首，周延民   

  1. 马遥，女，1988年生，吉林省集安市人，汉族，吉林大学口腔医学院在读硕士，主要从事口腔种植学研究。  
  • 出版日期:2015-05-21 发布日期:2015-05-21
  • 通讯作者: 周延民，教授、主任医师，吉林大学口腔医院种植科， 吉林省长春市 130021
  • 作者简介:马遥，女，1988年生，吉林省集安市人，汉族，吉林大学口腔医学院在读硕士，主要从事口腔种植学研究。
  • 基金资助:

    吉林省科技发展计划项目(20100441)

Poly(butylene succinate)/polypropylenecarbonate biofilms: preparation and performanc

Ma Yao, Chu Shun-li, Sun Yue, Ma Shan-shan, Li Xue, Zhang Tian-shou, Zhou Yan-min   

  1. Ma Yao, Studying for master’s degree, Implantology Center, Stomatological School of Jilin University, Changchun 130021, Jilin Province, China
  • Online:2015-05-21 Published:2015-05-21
  • Contact: Zhou Yan-min, Professor, Chief physician, Implantology Center, Stomatological School of Jilin University, Changchun 130021, Jilin Province, China
  • About author:Ma Yao, Studying for master’s degree, Implantology Center, Stomatological School of Jilin University, Changchun 130021, Jilin Province, China
  • Supported by:

    the Science and Technology Development Plan of Jilin Province, No. 20100441

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被引次数

1

摘要：

背景：聚丁二酸丁二醇酯、聚碳酸亚丙酯是近年来发展的新型医用材料，具有良好的生物相容性、可降解性及价格低廉等优点。
目的：采用静电纺丝法制备聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜，评价其理化性能、体外降解性能及对细胞增殖的影响。
方法：采用静电纺丝法制备聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜：常温下称取聚丁二酸丁二醇酯、聚碳酸亚丙酯各0.9 g，溶解于10 mL三氯甲烷中，磁力搅拌，待其充分溶解后，将纺丝液加入纺丝管内，调整纺丝距离约15 cm，最后开启电源将电压调至18 kV即可，扫描电镜观察其表面形态，检测其强度、接触角、吸水率及体外降解过程中的pH值和失重率。将人骨肉瘤细胞MG63与生物膜共培养7 d后，采用CCK-8检测细胞增殖。
结果与结论：扫描电镜可见聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜呈均匀多孔状，孔孔交通，纤维直径约  0.88 μm，平均孔径约5.68 μm，孔隙率为78.3%，断裂强度平均为2.31 MPa，断裂伸长率为23.48%，接触角约为87°，吸水率为68.54%；生物膜降解过程中pH值呈缓慢降低，第12周时pH值为6.76，同时该生物膜处于均匀缓慢的降解过程中，12周时失重率为6.04%。CCK-8实验结果显示该生物膜对细胞的增殖具有一定的促进作用。表明静电纺丝聚丁二酸丁二醇酯/聚碳酸亚丙酯生物膜具有良好的理化性能，具备良好的空间维持能力、湿润性及降解性，能为骨组织再生提供充足的时间。

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 生物材料, 骨生物材料, 聚丁二酸丁二醇酯, 聚碳酸亚丙酯, 生物膜, 骨缺损, 引导骨再生

Abstract:

BACKGROUND: Poly(butylene succinate) (PBS) and polypropylenecarbonate (PPC) are new medical materials developed in recent years, characterized as good biocompatibility, biodegradability and the low price.
OBJECTIVE: To prepare the PBS/PPC biofilm by electrostatic spinning method and evaluate its physical and chemical properties, degradation performance and effect on cell proliferation in vitro.
METHODS: The PBS/PPC biofilm was prepared using electrostatic spinning method: 0.9 g PBS and 0.9 g PPC were dissolved in 10 mL of trichloromethane at room temperature and stirred magnetically until they were fully 
dissolved. Then, the spinning solution was added into a spinning tube with a distance of about 15 cm and at a voltage of 18 kV. The surface morphology was observed by scanning electron microscopy. The intensity, contact angle and water absorption, pH value and weight loss in the process of in vitro degradation were measured. MG63 cells were co-cultured with the biofilm for 7 days and cell proliferation was detected by cell counting kit-8.
RESULTS AND CONCLUSION: The PBS/PPC biofilm showed a porous structure with interconnected pores. The fiber diameter was about 0.88 μm, the average aperture was about 5.68 μm, the porosity was 78.3%, the fracture intensity was 2.31 MPa, the elongation rate at break was 23.48%, the average value of contact angle was 87°, and the water absorption rate was 68.54%. During the biofilm degradation, the pH value decreased gradually andreduced to 6.76 at 12 weeks; meanwhile, the biofilm degraded equally and gradually, and the weight loss rate was 6.04% at the end of the 12th week. The results of cell counting kit-8 showed that the PBS/PPC biofilm could promote cell proliferation. Overall, the PBS/PPC biofilm has good physical and chemical properties, good space-making feature, wettability and degradability, which can provide sufficient time for bone tissue regeneration.

Key words: Biocompatible Materials, Biofilms, Bone Regeneration

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