中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (43): 6951-6958.doi: 10.3969/j.issn.2095-4344.2014.43.011

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

温度敏感性水凝胶携带阿利吉仑对大鼠急性心肌梗死后心功能的影响

陈盼盼，李晓艳，蒋学俊，刘  杨   

  1. 武汉大学人民医院，湖北省武汉市  430000
  • 收稿日期:2014-09-02 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 李晓艳，主任医师，武汉大学人民医院，湖北省武汉市 430000
  • 作者简介:陈盼盼，女，1988年生，河南省南阳市人，武汉大学第一临床学院在读硕士，主要从事高血压、冠心病等方面的研究。
  • 基金资助:

    国家自然科学基金青年项目(81170307)

Thermosensitive hydrogel with Aliskrien influence the heart function of rats with acute myocardial infarction

Chen Pan-pan, Li Xiao-yan, Jiang Xue-jun, Liu Yang   

  1. Renmin Hospital of Wuhan University, Wuhan 430000, Hubei Province, China
  • Received:2014-09-02 Online:2014-10-15 Published:2014-10-15
  • Contact: Li Xiao-yan, Chief physician, Renmin Hospital of Wuhan University, Wuhan 430000, Hubei Province, China
  • About author:Chen Pan-pan, Studying for master’s degree, Renmin Hospital of Wuhan University, Wuhan 430000, Hubei Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81170307

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摘要：

背景：前期实验已证明温度敏感性水凝胶安全无毒，具有良好的生物相容性，且有一定的心脏保护作用，可作为药物载体局部注射于心肌组织，以避免全身给药产生的不良反应。
目的：观察温度敏感性水凝胶携带阿利吉仑对急性心肌梗死后大鼠心功能、心肌细胞凋亡及心室重构的影响。
方法：将70只Wistar大鼠随机分为假手术组(n=10)、PBS组(n=15)、PBS+药组(n=15)、水凝胶组(n=15)、水凝胶+药组(n=15)，后4组建立急性心肌梗死模型，随后分别在梗死心肌边界注射PBS、含阿利吉仑的PBS溶液、水凝胶、含阿利吉仑的水凝胶。术后28 d进行相关检测。
结果与结论：与假手术组比较，其余4组心功能及金属蛋白酶抑制因子1水平下降(P < 0.05)，心肌细胞凋亡、基质金属蛋白酶2、基质金属蛋白酶9、转化生长因子β、Ⅰ型及Ⅲ型胶原水平增高(P < 0.05)；PBS组、PBS+药组、水凝胶组血管紧张素Ⅰ及血管紧张素Ⅱ水平升高(P < 0.05)，水凝胶+药组血管紧张素Ⅰ及血管紧张素Ⅱ水平降低(P < 0.05)。水凝胶+药组心功能及金属蛋白酶抑制因子1水平优于PBS组、PBS+药组、水凝胶组(P < 0.05)，心肌细胞凋亡、基质金属蛋白酶2、基质金属蛋白酶9、转化生长因子β、Ⅰ型及Ⅲ型胶原水平低于PBS组、PBS+药组、水凝胶组(P < 0.05)。表明温度敏感性水凝胶携带阿利吉仑可进一步加强心脏保护作用，减少细胞凋亡，抑制心肌纤维化，延缓心肌梗死后心室重塑的发展。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 缓释材料, 水凝胶, 阿利吉仑, 心肌梗死, 心功能, 细胞凋亡, 心室重塑, 国家自然科学基金

Abstract:

BACKGROUND: Previous experiments have shown that the thermosensitive hydrogel is safe, nontoxic, biocompatible, and has a certain cardioprotective effect, which can be used as a drug carrier injected into the myocardial tissue, in order to avoid adverse reactions produced by systemic administration.
OBJECTIVE: To observe the influence of thermosensitive hydrogel with Aliskrien on heart function, apoptosis and ventricular remodeling of rats with acute myocardial infarction.
METHODS: Totally 70 Wistar rats were randomized into sham group (n=10), PBS group (n=15), PBS+Aliskrien group (n=15), hydrogel group (n=15), hydrogel+Aliskrien group (n=15). Models of acute myocardial infarction were established in the latter four groups. After modeling, PBS, PBS with Aliskrien, hydrogel, and hydrogel with Aliskrien were respectively injected into the border of infracted myocardium. After 28 days, relevant measurements were performed.
RESULTS AND CONCLUSION: Compared with the sham group, the heart function and expression of tissue inhibitor of metalloproteinase-1 were decreased significantly in the other four groups (P < 0.05), while the number of apoptotic cells, matrix metalloproteinase-2, matrix metalloproteinase-9, transforming growth factor β, collagen type I and III were increased (P < 0.05). The levels of angiotensin I and angiotensin II were higher in the PBS, PBS+Aliskrien, hydrogel groups than the sham group (P < 0.05), while they were decreased in the hydrogel+ 
Aliskrien group than the sham group (P < 0.05). The level of tissue inhibitor of metalloproteinase-1 and heart function in the hydrogel+Aliskrien group were superior to those in the PBS, PBS+Aliskrien, and hydrogel groups (P < 0.05), while the number of apoptotic cells, the levels of metalloproteinase-2, matrix metalloproteinase-9, transforming growth factor β, collagen type I and III were lower than those in the PBS, PBS+Aliskrien, and hydrogel groups (P < 0.05). These findings indicate that as a new type of sustained-release formulation, thermosensitive hydroge with Aliskrien could further improve the heart function, decrease cell apoptosis and inhibit myocardial fibrosis, and delay ventricular remodeling after myocardial infarction.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: hydrogel, myocardial infarction, apoptosis

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