中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (27): 4406-4411.doi: 10.3969/j.issn.2095-4344.2014.27.023

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

比较2种常用授精法在体外授精-胚胎移植中的差异

罗灿峤，李宇彬，李  涛，肖  珊，龙玲莉，谢文林，聂钊铭   

  1. 中山大学附属第一医院，广东省广州市   510000
  • 出版日期:2014-06-30 发布日期:2014-06-30
  • 通讯作者: 李宇彬，博士，主治医师，中山大学附属第一医院，广东省广州市 510000
  • 作者简介:罗灿峤，男，1971年生，广东省廉江市人，汉族，2004年广州医科大学毕业，副主任技师，主要从事病理生理基础研究。
  • 基金资助:

    国家自然科学青年基金(81100470，81270750)；中山大学青年教师培育项目(12ykpy24)；广东省自然科学基金资助项目(S2012010009390)

Comparison of two common insemination methods on in-vitro fertilization and embryo transfer

Luo Can-qiao, Li Yu-bin, Li Tao, Xiao Shan, Long Ling-li, Xie Wen-lin, Nie Zhao-ming   

  1. The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China
  • Online:2014-06-30 Published:2014-06-30
  • Contact: Li Yu-bin, M.D., Attending physician, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China
  • About author:Luo Can-qiao, Associate chief technician, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81100470, 81270750; Young Teachers Cultivation Project of Sun Yat-sen University, No. 12ykpy24; the Natural Science Foundation of Guangdong Province, No. S2012010009390

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摘要：

背景：体外授精-胚胎移植技术目前广泛应用于人类助孕，助孕成功率稳步上升，卵子体外授精成功率直接影响到最终的助孕结局。
目的：评估微滴法与敞开式授精法这2种常用授精方法在常规体外授精中卵子受精以及胚胎发育方面差异。
方法：募集因非男性因素行体外受精助孕的1 175例患者进行了一项随机研究，对比这2种授精方法的有效性。其中573例患者进行了微滴法授精；另外602例患者进行了敞开式授精。然后比较这两种授精方式对卵子授精及胚胎质量的影响。
结果与结论：微滴法受精失败率[完全不受精率+低受精率(受精卵子<25%)]比敞开式授精要高(11.9%，3.3%，P < 0.001)。然而优质胚胎率、临床妊娠率在两组间差异无显著性意义(P均 > 0.05)。结果表明敞开式授精法是一种相对简单而且授精失败率低的良好授精方法。但受精是过程复杂，内外影响因素众多，需要进行更细致观察，以确认这两种授精方法在体外授精-胚胎移植中的差异。



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

关键词: 组织构建, 精子, 授精, 卵子, 体外授精, 受精失败, 妊娠率, 国家自然科学基金

Abstract:

BACKGROUND: The in-vitro fertilization and embryo transfer technique has been widely applied in human insemination. The rate of successful insemination is gradually rising, and the in-vitro fertilization directly determine the insemination outcome.
OBJECTIVE: To evaluate the difference between the two common using insemination methods, microdrop and open, in in-vitro fertilization and embryo development.
METHODS: A randomized study was conducted to compare microdrop and open insemination methods among non-male factor patients undergoing in-vitro fertilization and embryo transfer. A total of 1 175 cases were enrolled in the research. There were 573 cases in the microdrop group, and 602 cases in open insemination group. The fertilization rate and embryo development in the two groups were compared.
RESULTS AND CONCLUSION: The fertilization failure rate [total fertilization failure rate + low fertilization rate(< 25% oocytes fertilized)] in the microdrop insemination group was higher than in the open insemination group (11.9%, 3.3%, P < 0.001), while the good quality embryo rate and pregnancy rate did not differ significantly between the two groups (all P > 0.05). The open insemination method is a simple insemination method with a lower fertilization failure rate. As the fertilization is a highly complicated process involving many extrinsic and intrinsic factors, further study is needed to confirm the effects of the two insemination methods on in-vitro fertilization outcome.



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

Key words: sperm, fertilization, in vitro, pregnancy rate

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