中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (37): 6541-6547.doi: 10.3969/j.issn.2095-4344.2013.37.001

• 骨组织构建 bone tissue construction •    下一篇

不同时期转染基因对牵引区新骨生成的影响

胡纯兵1，吴国平1，刘  希2，兰永树3，何小川1，郭  力1   

  1. 泸州医学院附属医院，1整形外科，3CT室，四川省泸州市  646000；2泸州医学院附属口腔医院放射科，四川省泸州市  646000
  • 收稿日期:2013-04-09 修回日期:2013-04-28 出版日期:2013-09-10 发布日期:2013-09-10
  • 通讯作者: 吴国平，博士，教授，泸州医学院附属医院整形外科，四川省泸州市 646000 drgpwu@yahoo.com.cn
  • 作者简介:胡纯兵★，男，1983年生，四川省西昌市人，汉族，2011年泸州医学院毕业，硕士，医师，主要从事颅颌面外科整形研究。 huchunbingdr@163.com
  • 基金资助:

    国家自然科学基金资助项目(30600653)*，四川省教育厅重点项目(11ZA239)*，四川省卫生厅科研课题(100285)*

Gene transfection at different time affects the osteogenesis in traction area

Hu Chun-bing1, Wu Guo-ping1, Liu Xi2, Lan Yong-shu3, He Xiao-chuan1, Guo Li1   

  1. 1Department of Plastic Surgery, 3CT Room, the Affiliated Hospital of Luzhou Medical College, Luzhou  646000, Sichuan Province, China; 2Department of Radiology, Hospital of Stomatology, Luzhou Medical College, Luzhou  646000, Sichuan Province, China
  • Received:2013-04-09 Revised:2013-04-28 Online:2013-09-10 Published:2013-09-10
  • Contact: Wu Guo-ping, M.D., Professor, Department of Plastic Surgery, the Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China drgpwu@yahoo.com.cn
  • About author:Hu Chun-bing★, Master, physician, Department of Plastic Surgery, the Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China huchunbingdr@163.com
  • Supported by:

    National Natural Science Foundation of China, No. 30600653*; Key Project of Sichuan Provincial Education Department, No. 11ZA239*; Scientific Research Project of Sichuan Provincial Health Department, No. 100285*

PDF

393

被引次数

2

摘要：

背景：前期研究表明,电穿孔介导的重组质粒pIRES-hBMP2-hVEGF165能促进牵引区早期新生血管的形成和新骨形成。
目的：观察不同时机转染基因对兔下颌骨牵张成骨过程中牵引区新骨生成的影响，探索基因导入的最佳转染时间，以获得更好的治疗效果。
方法：新西兰大白兔48只，全麻下行双侧下颌骨截骨及牵引器植入后，采用随机区组法分成4组，分别于造模后即刻、牵引开始时、牵引结束时在双侧牵引区注射2 μg(0.1 g/L)重组质粒pIRES-hBMP2-hVEGF165，3组均予电穿孔刺激；单纯牵引组单纯牵引不行基因转染。各组于造模后3 d开始以0.8 mm/d、1次/d的速率进行牵引，连续牵引10 d；各组分别于固定期1，2，4，8周处死3只兔子，切取下颌牵引区新生组织行组织学检测和形态计量学分析。
结果与结论：组织学检查和形态计量学分析发现，牵引期转染组与即刻转染组、固定期转染组、单纯牵引组比较间隙内有更多的新生血管、成骨细胞和间充质细胞等成分，各时点新生骨量与新生骨小梁宽度明显高于后者(P < 0.05)。表明在牵引开始时(牵引期)进行基因转染较其他时间转染促进新骨生成作用明显，能够获得最佳的促进新骨生成的效果，提示牵引期是下颌骨基因治疗的最佳时机。

关键词: 组织构建, 骨组织构建, 电穿孔, 基因疗法, 牵引成骨, 转染基因, 转染时间, 组织形态计量学, 新骨生成, 国家自然科学基金

Abstract:

BACKGROUND: The previous studies have shown that electroporation-mediated recombinant plasmid pIRES-hBMP2-hVEGF165 can promote the early angiogenesis and new bone formation in the traction zone.
OBJECTIVE: To investigate the effect of gene transfection at different time on the new bone formation in the traction zone during mandibular distraction in rabbits, so as to explore the optimal time for gene therapy and obtain a better treatment effect.
METHODS: Forty-eight New-Zealand rabbits were employed. After accomplished the bilateral mandibular osteotomy and distractor implantation under general anaesthesia, the rabbits were randomly divided into four groups. The bilateral traction zones were transfected with the 2 μg (0.1g/L) recombinant plasmids pIRES-hBMP2-hVEGF165 at latency period, traction period and consolidation period, respectively, and the rabbits in these three groups received electroporation stimulation. The simple traction group only received traction without gene transfection. The rabbits in each group received traction at 3 days after modeling with the rate of 0.8 mm/d and once per day, and continuous traction for 10 days; three rabbits were sacrificed in each group at 1, 2, 4 and 8 week. The new tissues in the mandibular traction zone were cut for histological observation and histomorphometry analysis.
RESULTS AND CONCLUSION: Histological examination and histomorphometry analysis showed that amounts of newly formed vessels, osteoblasts and mesenchymal cells of distraction period transfection group were greater than those of the latency period transfection group, consolidation period transfection group and simple traction group, and the new bone volume and the width of bone trabecula in the distraction period transfection group were higher than those in the other three groups (P < 0.05). It is better to transfect gene at the beginning of traction (distraction period) than at other stages of traction; in this way, we can obtain more remarkable effects on new bone formation. It suggests that the distraction stage is the optimal time for gene therapy.

Key words: electroporation, gene therapy, traction, transfection

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