中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (24): 4521-4528.doi: 10.3969/j.issn.2095-4344.2013.24.022

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

家族性肌萎缩侧索硬化小鼠保种过程中出现种系退化

蔡 宾，樊东升   

  1. 北京大学第三医院，北京市 100191
  • 收稿日期:2013-01-17 修回日期:2013-02-26 出版日期:2013-06-11 发布日期:2013-06-11
  • 通讯作者: 樊东升，博士，主任医师，教授，研究员，博士生导师，北京大学第三医院神经科，北京市 100191 dsfan@sina.com
  • 作者简介:蔡宾★，男，1986年生，河北省人，汉族，2013年北京大学医学部毕业，硕士，主要从事肌萎缩侧索硬化的临床与基础研究。 caibin@bjmu.edu.cn
  • 基金资助:

    国家自然科学基金(81030019，30871359)；北京市自然科学基金(7082099，7102161)；教育部博士点基金(20100001110084)；首都医学科技发展基金(SF-2009-III-34)

Germline degradation of a mouse model of familial amyotrophic lateral sclerosis when breeding

Cai Bin, Fan Dong-sheng   

  1. Peking University Third Hospital, Beijing 100191, China
  • Received:2013-01-17 Revised:2013-02-26 Online:2013-06-11 Published:2013-06-11
  • Contact: Fan Dong-sheng, M.D., Chief physician, Professor, Researcher, Doctoral supervisor, Peking University Third Hospital, Beijing 100191, China dsfan@sina.com
  • About author:Cai Bin★, Master, Peking University Third Hospital, Beijing 100191, China caibin@bjmu.edu.cn
  • Supported by:

    National Natural Science Foundation of China, No. 81030019*, 30871359*; Beijing Natural Science Foundation, No. 7082099*, 7102161*; Doctoral Fund of Ministry of Education, No. 20100001110084*; Capital Medical Science and Technology Development Fund, No. SF-2009-III-34*

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512

被引次数

1

摘要：

背景：SOD1-G93A转基因小鼠是研究肌萎缩侧索硬化的经典代表性疾病模型动物，然而对于这种转基因小鼠在保种过程中所存在的种系退化现象却少有报道。
目的：研究家族性肌萎缩侧索硬化小鼠在保种过程中出现的种系退化现象。
方法：通过比较家族性肌萎缩侧索硬化疾病模型SOD1-G93A转基因小鼠在保种过程中不同代间的怀孕胎数、产仔数以及子代鼠中雄性和阳性小鼠所占得比例分析该转基因小鼠保种过程中生育能力的变化，并比较不同代间小鼠的起病时间、生存期以分析该转基因小鼠在保种过程中发病情况的变化。
结果与结论：第3，6代小鼠与第0代比较，其怀孕胎数、每胎产仔数、每只产仔数等均显著下降(P < 0.05)，且第6代小鼠中有一半以上雌鼠无法受孕。其子代鼠中雄性小鼠以及阳性小鼠所占的比例有下降趋势，但是其差异无显著性意义。第6代小鼠与第1代比较其发病时间与死亡时间分别推迟了6.37 d (P=0.004)和9.67 d(P=0.022)，差异有显著性意义。以上结果显示家族性肌萎缩侧索硬化小鼠在保种过程中存在生育能力下降、发病及死亡时间推迟等种系退化现象。

关键词: 组织构建, 组织构建基础实验, 肌萎缩侧索硬化, 转基因小鼠, 疾病动物模型, 种系退化, 繁殖, 国家自然科学基金

Abstract:

BACKGROUND: SOD1-G93A transgenic mouse is a classical morbid animal model in the investigation of amyotrophic lateral sclerosis. However, there are few reports about the germline deterioration of this transgenic mouse model when breeding.
OBJECTIVE: To investigate the germline deterioration of mouse model of familial amyotrophic lateral sclerosis when breeding.
METHODS: The times of pregnancy, litter size among different generations and the ratios of male and positive of the filial generation were compared to analyze the change of fertility of amyotrophic lateral sclerosis mouse model of SOD1-G93A transgenic mouse when breeding. And the times of invasion and death were compared to analyze the change of pathogenesis of this transgenic mouse when breeding.
RESULTS AND CONCLUSION: The times of pregnancy, per litter size and per mice were decreased significantly (P < 0.05) when comparing the third and sixth generation with the first generation, and moreover there were over half of the mouse of the sixth generation who can not inbreed. The ratios of male and positive mouse of the filial generation also decreased, but the difference was not significant. The time of invasion and death delayed significantly by 6.37 days (P=0.004) and 9.67 days (P=0.022) respectively when comparing the sixth generation with the first generation, and the difference was significant. As a result, familial amyotrophic lateral sclerosis mouse showed deteriorated germline when breeding such as the fertility decline and the delay of the time of invasion and death.

Key words: tissue construction, basic experiments in tissue construction, amyotrophic lateral sclerosis, transgenic mouse, morbid animal model, germline deterioration, breeding, National Natural Science Foundation of China

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