中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (2): 286-290.doi: 10.3969/j.issn.2095-4344.2013.02.018

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

尿酸钠结晶影响肾小管上皮细胞的紧密连接

李慧娟,陈 敢,傅君舟,周姗姗   

  1. 广州医学院附属广州市第一人民医院,广东省广州市 510182
  • 收稿日期:2012-06-25 修回日期:2012-08-13 出版日期:2013-01-08 发布日期:2013-01-08
  • 通讯作者: 傅君舟,硕士,教授,主任医师, 广州市第一人民医院,广东省广州市 510182 fjzh@163.com
  • 作者简介:李慧娟★,女,1986年生,江西省九江市人,汉族,2012年广州医学院毕业,硕士,医师,主要从事尿酸性肾病方面的研究。hjli0968@163.com;huijuanli515@126.com

Monosodium urate monohydrate crystals affect the tight junctions among renal tubular epithelial cells

Li Hui-juan, Chen Gan, Fu Jun-zhou, Zhou Shan-shan   

  1. First People’s Hospital of Guangzhou City, Guangzhou Medical University, Guangzhou 510182, Guangdong Province, China
  • Received:2012-06-25 Revised:2012-08-13 Online:2013-01-08 Published:2013-01-08
  • Contact: Fu Jun-zhou, Master, Professor, Chief physician, First People’s Hospital of Guangzhou City, Guangzhou Medical University, Guangzhou 510182, Guangdong Province, China fjzh@163.com
  • About author:Li Hui-juan★, Master, Physician, First People’s Hospital of Guangzhou City, Guangzhou Medical University, Guangzhou 510182, Guangdong Province, China hjli0968@163.com, huijuanli515@126.com

摘要:

背景:目前研究来看,尿酸性结石与紧密连接蛋白及肾间质纤维化的关系仍未明确。
目的:观察尿酸钠结晶对肾小管上皮细胞紧密连接的影响。
方法:配制单钠尿酸钠晶体。将正常大鼠肾小管上皮细胞随机分为对照组和尿酸钠结晶组,分别用无血清培养基和尿酸钠结晶进行培养。免疫荧光和RT-PCR方法检测24,48,72 h紧密连接蛋白的表达。
结果与结论:与对照组比较,尿酸钠结晶于不同时相刺激NRK-52E细胞后,紧密连接蛋白和mRNA表达下降(P < 0.05),72 h时下降显著(P < 0.01),蛋白表达出现重新分布现象。说明尿酸钠结晶可破坏肾小管上皮细胞紧密连接蛋白的结构、功能及导致分布异常。

关键词: 组织构建, 组织构建细胞学实验, 尿酸钠结晶, 紧密连接, 紧密连接蛋白, 肾小管上皮细胞, NRK-52E细胞, 肾间质纤维化, 高尿酸血症, 跨膜蛋白, 膜周蛋白, 大鼠, 组织构建图片文章

Abstract:

BACKGROUND: Up to now, the relationship of uric acid stones with tight junction proteins and renal interstitial fibrosis is yet unclear.
OBJECTIVE: To observe the effect of monosodium urate (MSU) monohydrate crystals on the tight junction among NRK-52E cells.
METHODS: The NRK-52E cells were divided into two groups: control group and MSU group cultured in serum-free medium and MSU crystals, respectively. The protein and mRNA expressions of ZO-1 were investigated by immunofluorescence and reverse transcription-PCR, respectively. 
RESULTS AND CONCLUSION: Compared with the control group, the protein and mRNA expressions of ZO-1 were down-regulated after the stimulation of MSU at 24, 48, 72 hours (P < 0.05), especially at 72 hours (P < 0.01). Protein expression appeared with redistribution phenomenon. These findings indicate that MSU can damage the structure, function and distribution of tight junction proteins of renal tubular epithelial cells

Key words: tissue construction, cytological experiments of tissue construction, monosodium urate monohydrate crystals, tight junctions, claudin, tubular epithelial cells, NRK-52E cells, renal interstitial fibrosis, hyperuricemia, transmembrane protein, membranous protein, rats, tissue construction photographs-containing paper

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