中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8620-8624.doi: 10.3969/j.issn.2095-4344.2012.46.014

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

人牙龈成纤维细胞原代培养及冻存和复苏

惠 敏1，倪 莹2，郑 静3，刘 健4，王 帅5，张晓明6   

  1. 滨州医学院附属医院，1口腔内科，5放射科，6口腔修复与正畸科，山东省滨州市 256600；滨州医学院，2口腔学院，3临床试验中心，山东省滨州市 256603； 4日照市中医院口腔正畸科，山东省日照市 276800
  • 收稿日期:2012-01-05 修回日期:2012-04-06 出版日期:2012-11-11 发布日期:2012-11-11
  • 通讯作者: 张晓明，滨州医学院附属医院口腔修复与正畸科，山东省滨州市 256600
  • 作者简介:惠敏，女，1963年生，山东省滨州市人，汉族，2009年安徽理工大学毕业，实验师，主要从事固定修复体牙周支持组织受力分析、正畸性牙根吸收的研究。

Primary culture, cryopreservation and resuscitation of human gingival fibroblasts

Hui Min1, Ni Ying2, Zheng Jing3, Liu Jian4, Wang Shuai5, Zhang Xiao-ming6   

  1. 1Department of Stomatology, 5Department of Radiology, 6Department of Prosthodontics and Orthodontics, Affiliated Hospital of Binzhou Medical College, Binzhou 256600, Shandong Province, China; 2School of Stomatology, 3Clinical Test Registration Center, Binzhou Medical College, Binzhou 256603, Shandong Province, China; 4Department of Orthodontics, the Hospital of Traditional Chinese Medicine in Rizhao, Rizhao 276800, Shandong Province, China
  • Received:2012-01-05 Revised:2012-04-06 Online:2012-11-11 Published:2012-11-11
  • Contact: Zhang Xiao-ming, Department of Prosthodontics and Orthodontics, Affiliated Hospital of Binzhou Medical College, Binzhou 256600, Shandong Province, China
  • About author:Hui Min, Experimentalist, Department of Stomatology, Affiliated Hospital of Binzhou Medical College, Binzhou 256600, Shandong Province, China

PDF

565

被引次数

6

摘要：

背景：牙龈成纤维细胞是牙龈固有结缔组织层中主要的细胞，在许多生理和病理过程中起着重要作用。
目的：对人牙龈成纤维细胞进行原代培养、鉴定、冻存及复苏。
方法：采用组织块培养法培养人牙龈成纤维细胞，并进行形态学和免疫学鉴定。对人牙龈成纤维细胞进行冻存与复苏，倒置显微镜下观察细胞形态变化。
结果与结论：人牙龈成纤维细胞原代培养成功率为86.7%，细胞呈梭形或纺锤形。免疫组织化学鉴定抗波性蛋白抗体染色阳性，抗角蛋白抗体染色阴性，为中胚层来源的成纤维细胞。牙龈成纤维细胞冻存与复苏成功，细胞经2次传代后生物学性状与原代相似。提示采用组织块培养法培养原代人牙龈成纤维细胞及冻存与复苏方法可行。

关键词: 人牙龈成纤维细胞, 细胞培养, 冻存, 复苏, 鉴定, 组织构建

Abstract:

BACKGROUND: Human gingival fibroblasts (HGFs) are the main cells in the connective tissue layers, which play an important role in many physiological and pathological processes.
OBJECTIVE: To perform primary culture, identification, cryopreservation and resuscitation for HGFs.
METHODS: First, the HGFs were cultured by the methods of tissue culture and identified by morphological and immunocytochemical analysis. Next, HGFs were frozen and resuscitated. And then their morphological changes were observed by the inverted microscope.
RESULTS AND CONCLUSION: The success rate of HGFs from the primary cells was 86.7%, and the HGFs showed fusiform or spindle-shaped. The immunochemistry study indicated that vimentin showed positive staining; while cytokeratin showed negative staining, which originated from mesoblastma. The cryopreservation and resuscitation of HGFs were successful. The biological character of HGFs after second passage was similar to that of the original generation. These results suggest that the primary culture, cryopreservation and resuscitation of HGFs can be feasible by using the method of tissue culture.

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