中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8611-8614.doi: 10.3969/j.issn.2095-4344.2012.46.012

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

兔关节软骨接受体外冲击波治疗后细胞增殖及Ⅱ型胶原的表达

王明波1，赵 喆2，云文科1   

  1. 1武警内蒙古总队医院外二科，内蒙古自治区呼和浩特市 010020
    2解放军总医院骨科研究所，北京市 100039
  • 收稿日期:2012-01-09 修回日期:2012-04-07 出版日期:2012-11-11 发布日期:2012-11-11
  • 作者简介:王明波★，男，1981年生，内蒙古自治区赤峰市人，蒙古族，2009年河北医科大学毕业，硕士，医师，主要从事骨与关节损伤修复的研究。 wangmingbo_2003@yahoo.com.cn

Effect of extracorporeal shock wave on the proliferation of rabbit articular chondrocytes and type Ⅱ collagen expression

Wang Ming-bo1, Zhao Zhe2, Yun Wen-ke1   

  1. 1Department of Second Surgery, Armed Police Corps Hospital of Inner Mongolia, Hohhot 010020, Inner Mongolia Autonomous Region, China
    2Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100039, China
  • Received:2012-01-09 Revised:2012-04-07 Online:2012-11-11 Published:2012-11-11
  • About author:Wang Ming-bo★, Mater, Physician, Department of Second Surgery, Armed Police Corps Hospital of Inner Mongolia, Hohhot 010020, Inner Mongolia Autonomous Region, China wangmingbo_2003@yahoo.com.cn

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370

被引次数

4

摘要：

背景：关节软骨损伤后自身修复能力有限，体外冲击波可能提供一种高质量的修复关节软骨损伤并达到很好远期疗效的方法。
目的：探讨体外冲击波对兔关节软骨细胞增殖及Ⅱ型胶原蛋白表达的影响。
方法：酶消化法获得正常兔膝关节软骨细胞，培养并传代，实验A、B、C组分别用0.5×105，1.5×105，2.5×105 Pa等3种强度能量体外冲击波干预，空白对照组无任何干预措施。
结果与结论：细胞生长曲线在第6天实验B组显著高于其他各组(P < 0.05)；ELISA法检测实验B组Ⅱ型胶原A值与其他各组相比，显著升高(P < 0.05)；RT-PCR法检测实验B组Ⅱ型胶原表达明显增强，与其他各组相比差异有显著性意义(P < 0.05)。提示适当能量强度及频次的体外冲击波刺激，能够明显促进软骨细胞的增殖及Ⅱ型胶原表达。

关键词: 体外冲击波, 软骨细胞, 细胞增殖, Ⅱ型胶原, 兔

Abstract:

BACKGROUND: Injured articular cartilage has a very limited self-repair ability. Extracorporeal shock wave may provide a method that has high-quality repair of articular cartilage defects and can achieve good late outcomes.
OBJECTIVE: To explore the effect of extracorporeal shock wave on the proliferation of rabbit articular chondrocytes and type Ⅱ collagen expression.
METHODS: Normal rabbit knee joint chondrocytes were obtained by enzymatic digestion method. The cells were cultured, passaged, and then randomized into three experimental groups: A, B and C groups were given 0.5×105, 1.5×105 and 2.5×105 Pa extracorporeal shock wave intervention, respectively. The blank control received no intervention.
RESULTS AND CONCLUSION: The cell growth curve in the group B at day 6 was higher than that in the other groups (P < 0.05). Enzyme-linked immunosorbent assay showed that compared with the other groups, the absorbance of type Ⅱ collagen in the group B was significantly increased (P < 0.05). Reverse transcription PCR indicated that type Ⅱ collagen expression was enhanced obviously in the group B, as compared with the other groups (P < 0.05). These results suggest that the extracorporeal shock wave stimulation of appropriate energy intensity and frequency can significantly promote chondrocyte proliferation and type Ⅱ collagen expression.

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