中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8583-8588.doi: 10.3969/j.issn.2095-4344.2012.46.007

• 骨组织构建 bone tissue construction • 上一篇    下一篇

延边地区膝骨性关节炎患者滑膜组织基因谱的表达

任红革1，李 林1，李振浩1，孙培磊1，崔逢德2   

  1. 1延边大学附属医院骨1科，吉林省延吉市 133000
    2延边大学医学部，吉林省延吉市 133000
  • 收稿日期:2012-06-29 修回日期:2012-10-24 出版日期:2012-11-11 发布日期:2012-11-11
  • 通讯作者: 李林，教授，延边大学附属医院骨1科，吉林省延吉市 133000
  • 作者简介:任红革★，男，1975年生，吉林省和龙市人，朝鲜族，1998年延边大学医学院毕业，硕士，副主任医师，主要从事骨关节疾病与组织工程研究。 Renhg196@sina.com

Gene expression profile in the synovium of patients with knee osteoarthritis in Yanbian area

Ren Hong-ge1, Li Lin1, Li Zhen-hao1, Sun Pei-lei1, Cui Feng-de2   

  1. 1First Department of Orthopedic Surgery, Yanbian University Hospital, Yanji 133000, Jilin Province, China
    2Yanbian University Medical College, Yanji 133000, Jilin Province, China
  • Received:2012-06-29 Revised:2012-10-24 Online:2012-11-11 Published:2012-11-11
  • Contact: Li Lin, Professor, First Department of Orthopedic Surgery, Yanbian University Hospital, Yanji 133000, Jilin Province, China lilin@yahoo.com
  • About author:Ren Hong-ge★, Master, Associate chief physician, First Department of Orthopedic Surgery, Yanbian University Hospital, Yanji 133000, of Jilin Province, China renhg196@sina.com

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508

被引次数

3

摘要：

背景：原发性骨性关节炎的发展是多种相关基因表达失常的缘故。采用mRNA基因表达谱芯片技术，检测膝关节骨性关节炎滑膜差异表达基因，有助于阐明骨性关节炎病理过程的相关机制。
目的：比较延边地区膝关节骨性关节炎和正常关节滑膜的基因表达谱变化，筛选出与膝关节骨性关节炎相关的差异表达基因，并探讨其在骨性关节炎发病机制中的意义。
方法：选择延边地区原发性膝关节骨性关节炎患者9例及正常对照者3例，应用mRNA基因表达谱芯片技术检测所获得的滑膜组织中表达差异的基因，筛选出表达差异基因并对其进行GO分析。
结果与结论：膝关节骨性关节炎及正常对照者滑膜的差异表达基因分析结果显示，延边地区膝关节骨关节炎滑膜中的差异表达基因共有1 261个，其中上调的基因有451个，下调的基因有810个。差异表达基因GO统计分析显示，信号转导、翻译调节、趋化作用、炎症应答、细胞增殖等多种基因的表达有差异。基因芯片技术能有效地筛选出骨性关节炎差异表达的基因，并进一步证实了骨性关节炎的病理过程是多种功能基因参与的复杂的病理过程。

关键词: 骨性关节炎, 基因芯片, 膝关节, 滑膜, 信号转导, 翻译调节, 趋化作用, 炎症应答, 细胞增殖, 差异表达

Abstract:

BACKGROUND: The progression of primary osteoarthritis is the result of abnormal expression of various genes. We use mRNA gene chip technique to detect the synovial gene expression of knee osteoarthritis, which provides evidence for related pathological mechanism of knee osteoarthritis.
OBJECTIVE: To compare the alteration of synovial gene expression in patients with knee osteoarthritis and normal people in Yanbian area, and to evaluate the potential osteoarthritis related discriminating genes and their roles in pathological mechanism.
METHODS: This study included nine patients with knee osteoarthritis and three healthy controls. mRNA gene chip technique was used to analyze the gene expression profile of synovial tissue in these subjects and GO analysis was performed.
RESULTS AND CONCLUSION: The results of gene expression in the synovial tissue of osteoarthritis patients and healthy controls group showed that 1 261 genes were differently expressed, including 451 up-regulated genes and 810 down-regulated genes. The GO analysis showed that the gene expression about signal transduction, regulation of transcription, chemotaxis, inflammatory response, and cell proliferation was different. Gene chip technique is effective for screening associated genes, and the pathological progression of osteoarthritis is a complex progression which many genes are involved.

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