中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8419-8423.doi: 10.3969/j.issn.2095-4344.2012.45.011

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

脐带来源间充质干细胞在成脂培养条件下骨形态发生蛋白2的表达

池 颖，戎丽娟，徐方运，韩之波，杨少光，王有为，马凤霞，卢士红，韩忠朝   

  1. 中国医学科学院血液学研究所血液病医院实验血液学国家重点实验室，天津市 300020
  • 收稿日期:2012-01-26 修回日期:2012-03-03 出版日期:2012-11-04 发布日期:2012-11-04
  • 通讯作者: 韩忠朝，博士，教授，中国医学科学院血液学研究所血液病医院实验血液学国家重点实验室，天津市 300020 hanzhongchao@hotmail.com
  • 作者简介:池颖★，女，1980年生，天津市人，汉族，2010年北京协和医学院毕业，硕士，助理研究员，主要从事成体干细胞方面的研究。 caizhuying@126.com

Bone morphogenetic protein 2 expression in umbilical cord mesenchymal stem cells after adipogenic induction

Chi Ying, Rong Li-juan, Xu Fang-yun, Han Zhi-bo, Yang Shao-guang, Wang You-wei, Ma Feng-xia, Lu Shi-hong, Han Zhong-chao   

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China
  • Received:2012-01-26 Revised:2012-03-03 Online:2012-11-04 Published:2012-11-04
  • Contact: Han Zhong-chao, Doctor, Professor, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China hanzhongchao@ hotmail.com
  • About author:Chi Ying★, Master, Associate professor, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China caizhuying@ 126.com

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摘要：

背景：脐带间充质干细胞在成脂培养条件下成脂相关信号因子过氧化物酶体增殖体激活受体γ的表达水平增高，同时成骨相关信号因子骨形态发生蛋白的表达水平也增高。
目的：检测成脂诱导条件下脐带间充质干细胞的过氧化物酶体增殖体激活受体γ及骨形态发生蛋白2的表达水平变化，并对结果进行初步探讨。
方法：取脐带来源的间充质干细胞，以成脂诱导体系对细胞进行诱导分化培养，倒置显微镜下观察细胞体外培养生长情况；油红O染色法观察成脂现象，茜素红及von kossa染色观察是否有沉淀形成；荧光定量PCR方法检测成脂相关因子过氧化物酶体增殖体激活受体γ及骨形态发生蛋白2的表达变化情况。
结果与结论：获得脐带来源间充质干细胞，并成功进行了成脂诱导分化。荧光定量PCR检测发现在成脂诱导条件下脐带间充质干细胞的过氧化物酶体增殖体激活受体γ和骨形态发生蛋白2的表达水平全都呈升高趋势，但是前者对分化方向的调控起主要作用。

关键词: 脐带间充质干细胞, 成脂分化, 过氧化物酶体增殖体激活受体γ, 骨形态发生蛋白2, 调控

Abstract:

BACKGROUND: Expression of bone morphogenetic protein 2 and peroxisome proliferator-activated receptorγgene in umbilical cord mesenchymal stem cells after adipogenic induction is increased at the same time.
OBEJECTIVE: To investigate the changes of bone morphogenetic protein 2 and peroxisome proliferator-activated receptorγgene expression in umbilical cord mesenchymal stem cells after adipogenic induction, and to compare the results.
METHODS: With specific inductive medium, umbilical cord mesenchymal stem cells were obtained and then induced into adipogenisis. Their morphological characteristics were observed with inverted microscope. Oil red O staining was used to observe the adipogenic phenomenon, and von kossa staining and alizarin red staining were used to identify the formation of precipitation. Real-time polymerase chain reaction was used to quantify the gene expression of bone morphogenetic protein 2 and peroxisome proliferator-activated receptor γ at various time points after adipogenic induction.
RESULTS AND CONCLUSION: Umbilical cord mesenchymal stem cells could be isolated and cultured successfully. After cultured in adipogenic medium, the cells differentiated into adipogenisis. The result of real-time polymerase chain reaction showed that both the expression levels of bone morphogenetic protein 2 and peroxisome proliferator-activated receptorγgene were increased after adipogenic induction, but peroxisome proliferator-activated receptorγplayed a major role in the regulation of differentiation.

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