中国组织工程研究

中国组织工程研究

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

贵金属烤瓷合金与高糖对细胞外液炎性因子的影响

谢 晖，丁江峰   

  1. 广州医学院口腔医院，广东省广州市 510140
  • 收稿日期:2012-04-30 修回日期:2012-08-01 出版日期:2012-10-21 发布日期:2012-10-21
  • 作者简介:谢晖★，女，1974年生，湖北省天门市人， 2010年山西医科大学毕业，硕士，主治医师，主要从事口腔修复学临床及基础的研究工作。 xiehui1317@163.com

Effects of noble ceramic alloy and high glucose on inflammatory factors in the extracellular fluid

Xie Hui, Ding Jiang-feng   

  1. The Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, Guangdong Province, China
  • Received:2012-04-30 Revised:2012-08-01 Online:2012-10-21 Published:2012-10-21
  • About author:Xie Hui★, Master, Attending physician, the Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, Guangdong Province, China xiehui1317@163.com

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382

被引次数

1

摘要：

背景：前期研究中发现高糖与烤瓷合金浸提液对小鼠成纤维细胞L-929的增殖存在交互效应。
目的：观察3种贵金属烤瓷合金与高糖对小鼠成纤维细胞L-929中白细胞介素6水平的影响。
方法：采用两因素析因设计实验研究不同糖浓度(11.1，22.2，33.3 mmol/L)下，89%金合金、74%金合金及银钯合金贵金属烤瓷合金浸提液，分别与小鼠成纤维细胞L-929体外共培养24h后，细胞外液中白细胞介素6水平的变化，并设未加入合金材料的空白对照为阴性对照组。
结果与结论：两因素析因设计分析结果表明，贵金属烤瓷合金浸提液的主效应有统计学意义(P=0.001)，其中74%金合金与银钯合金白细胞介素6水平低于阴性对照组(P=0.004，0)；糖浓度的主效应也有统计学意义(P=0.005)， 33.3 mmol/L糖浓度组白细胞介素6水平低于11.1 mmol/L(正常)糖浓度组(P=0.001)。但贵金属烤瓷合金浸提液与糖浓度两因素对小鼠成纤维细胞L-929细胞外液中白细胞介素6水平的影响无交互作用(P=0.33)。

关键词: 贵金属, 烤瓷合金, 银钯合金, 金合金, 浸提液, 细胞外液炎性因子, 高糖浓度, 交互作用, 小鼠成纤维细胞

Abstract:

BACKGROUND: The preliminary studies have found that there is an interaction effect of high sugar and ceramic alloy extracts on proliferation of L-929 mouse fibroblasts.
OBJECTIVE: To observe the effects of three kinds of noble ceramic alloys and high glucose on secrets of interleukin-6 from L-929 cells.
METHODS: Using two-factor factorial design experiments, we detected the changes of interleukin-6 levels in the extracellular fluid after co-culture of 89% Au-alloy, 74% Au-alloy and Ag-Pd alloy with mouse L-929 cells under different glucose concentrations (11.1, 22.2, 33.3 mmol/L). Cells without alloy served as negative controls.
RESULTS AND CONCLUSION: Two-factor factorial design results showed that the main effect of noble ceramic alloy extracts was statistically significant (P=0.001). The interleukin-6 levels of the supernatant in the 74% Au-alloy and the Ag-Pd alloy were significantly lower than that in the negative group (P=0.004, 0). The main effect of glucose concentration was also of statistical significance. The interleukin-6 level in supernatant in 33.3 mmol/L high glucose was significantly lower than that in 11.1 mmol/L glucose. However, no interaction existed between different extracts of noble ceramic alloys and glucose concentrations about the secrets of interleukin-6 from L-929 cells (P=0.33).

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